Breeding of a tomato genotype readily accessible to genetic manipulation

Abstract

A tomato genotype, superior in regenerating plants from cell cultures, was obtained by transferring regeneration capacity from Lycopersicon peruvianum into L. esculentum by classical breeding. This genotype, MsK93, greatly facilitates genetic manipulation of tomato, as was demonstrated by successful leaf disc transformation using Agrobacterium tumefaciens and by direct gene transer to protoplasts derived from callus.

Optimization of Direct Gene Transfer in Tomato

“…The origin and properties of the easily regenerable tomato genotype MSK93 has been previously described (3,4). The plasmids used and the DNA isolations are described by Jongsma et al (2).…”

“…The successful transformation of callus derived protoplasts of the tomato genotype MSK93 was recently described (2,4). Transformants were obtained with plasmid DNA containing the kanamycin resistance gene from Tn5 with expression signals from the nos gene as a selectable marker (plasmid pneoA18).…”

Avaliação da capacidade de regeneração in vitro em tomateiro industrial

“…Nessa espécie, a regeneração de plantas foi estabelecida há aproximadamente 20 anos (Kut et al, 1984;Sink & Reynolds, 1986), e métodos de regeneração têm sido relatados por diversos autores (Tan, 1987;McCormick, 1991;Redenbaugh et al, 1992;Wijbrandi & Both, 1993;Asakura et al, 1995;Fári et al, 1995a;Rhim et al, 1995). A partir de 1995, foram publicados diversos métodos sobre transformação genética via Agrobacterium tumefaciens e Agrobacterium rhizogenes (Shahin et al, 1986;Sukhapinda et al, 1987), bem como sobre transformação genética direta de protoplastos (Koornneef et al, 1986;Jongsma et al, 1987;Pieterse & Koornneef, 1988;Bellini et al, 1989). O tomateiro é uma das primeiras espécies, cujas cultivares transgênicas já foram comercializadas, principalmente no mercado americano (Redenbaugh et al, 1992).…”

Ac-induced disruption of the doubleDs structure in tomato

“…The constructed binary vectors were introduced into Agrobacterium tumefaciens LBA4404 (Hoekema et al 1983) by triparental mating (Fraley et al 1985). The tomato genotype Msk9 (Koornneef et al 1986) was transformed as described previously .…”