Optimization of Direct Gene Transfer in Tomato

Pieterse, C.M.J.; Koornneef, M.

doi:10.1007/978-94-009-2788-9_129

Cited by 5 publications

(4 citation statements)

References 8 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Tomato (Lycopersicon esculentum) has been the target for Agrobacterium -mediated transformation (McCormick et al 1986), direct DNA uptake into protoplasts (Pieterse & Koornneef 1988) and somatic hybridisation with a range of wild species, including L. peruvianum (Wijbrandi et al 1990) and L. chilense (Bonnema & O'Connell 1992). Central to successful genetic manipulation is the development of efficient protoplast-to-plant systems, but for tomato, this is particularly variety dependent.…”

mentioning

confidence: 99%

Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum)

Patil

Davey

Power

1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

mentioning

confidence: 99%

Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum)

Patil

Davey

Power

1994

Plant Cell Tiss Organ Cult

View full text Add to dashboard Cite

show abstract

“…Increases in transformation frequencies attributed to the addition of carrier DNA have been reported 15,10,18,20,25,29). The mode of action of carrier DNA is not understood.…”

Section: Carrier Dnamentioning

confidence: 94%

Direct gene transfer via polyethylene glycol

Johnson

Carswell

Shillito

1989

Journal of Tissue Culture Methods

View full text Add to dashboard Cite

show abstract

“…Transformation of tomato protoplasts has been performed using the calciumphosphate DNA transformation procedure (Koornneef et al ., 1986 ;Pieterse & Koornneef, 1988) . Selection for kanamycin resistance (50 mg/1) encoded by the plasmid DNA used in transformation, was started two weeks after protoplasts transformation .…”

Section: Direct Gene Transfermentioning

confidence: 99%

Tomato: a crop species amenable to improvement by cellular and molecular methods

et al. 1989

Self Cite

View full text Add to dashboard Cite

Tomato is a crop plant with a relatively small DNA content per haploid genome and a well developed genetics . Plant regeneration from explants and protoplasts is feasable which led to the development of efficient transformation procedures .In view of the current data, the isolation of useful mutants at the cellular level probably will be of limited value in the genetic improvement of tomato . Protoplast fusion may lead to novel combinations of organelle and nuclear DNA (cybrids), whereas this technique also provides a means of introducing genetic information from alien species into tomato . Important developments have come from molecular approaches . Following the construction of an RFLP map, these RFLP markers can be used in tomato to tag quantitative traits bred in from related species . Both RFLP's and transposons are in the process of being used to clone desired genes for which no gene products are known . Cloned genes can be introduced and potentially improve specific properties of tomato especially those controlled by single genes . Recent results suggest that, in principle, phenotypic mutants can be created for cloned and characterized genes and will prove their value in further improving the cultivated tomato .

show abstract

Optimization of Direct Gene Transfer in Tomato

Cited by 5 publications

References 8 publications

Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum)

Highly efficlent plant regeneration from mesophyll protoplasts of Indian field cultivars of tomato (Lycopersicon esculentum)

Direct gene transfer via polyethylene glycol

Tomato: a crop species amenable to improvement by cellular and molecular methods

Contact Info

Product

Resources

About