Breeding of Aspergillus oryzae mutant strain with a high level of glucoamylase activity and non-melanization

Iwama, Naoko; Muranaka, Yasuhito; Saito, Tomoaki; Ishida, Katsuaki; Tonouchi, Akio; Miyairi, Kazuo; Takeda, Kiyoshi

doi:10.6013/jbrewsocjapan1988.102.213

J.Brew.Soc.Japan

2007

DOI: 10.6013/jbrewsocjapan1988.102.213

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Breeding of Aspergillus oryzae mutant strain with a high level of glucoamylase activity and non-melanization

Naoko Iwama¹,

Yasuhito Muranaka²,

Tomoaki Saito³

et al.

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Copper ion (Cu2+) is involved in the transcription of the tyrosinase-encoding melB gene of Aspergillus oryzae in solid-state culture

Chen,

Tonouchi

2023

Bioscience, Biotechnology, and Biochemistry

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In Aspergillus oryzae, the tyrosinase-encoding gene melB causes undesirable browning of sake and sake lees. This gene is known to be expressed specifically in solid-state culture; however, its expression mechanisms remain unknown. Here, we evaluated the possible factors affecting the transcription of melB and found that the copper ion (Cu2+) significantly enhanced the transcription level of melB in solid-state culture.

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Copper ion (Cu2+) is involved in the transcription of the tyrosinase-encoding melB gene of Aspergillus oryzae in solid-state culture

Chen,

Tonouchi

2023

Bioscience, Biotechnology, and Biochemistry

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Comparison of Biochemical Properties of Wild Type Glucoamylases and Those of High Glucoamylase Mutant Strains of <i>Aspergillus oryzae</i>

Iwama

Saito

Ishida

et al. 2010

Trans. Mat. Res. Soc. Japan

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The properties of mutant glucoamylases（GlaBs） from Aspergillus oryzae H-1 and G-4 strains with a high level of GlaB activity were compared with those of native GlaBs from parental strains. Amino acid compositions of both mutant GlaBs were different from those of native GlaBs, with a particular decrease in Ser residue of mutant GlaBs. Moreover, N-linked suger chains of native and mutant GlaBs were considered a complex or hybrid type, and not a high mannose type. Because N-linked sugar chain of each GlaB mainly consisted of galactose and N-acetylglucosamine, there was little mannose. Since a little more galactose and N-acetylglucosamine combined per mannose were found in the N-linked suger chains of mutant GlaBs than of native GlaBs, this suggested that each N-linked sugar chain length of mutant GlaBs was possibly shorter. Native GlaBs were not deglycosylated with Endoglycosidase H, while both mutant GlaBs were deglycosylated. This result suggests N-linked suger chain structures of both mutant GlaBs changed from a complex type in native GlaBs to a hybrid type. In circular dichroism spectra analysis, Tm values of GlaBs from H-1 and G-4 strains were approximately 53℃ both, and thus similar to Tm of native GlaBs. Therefore, the thermostability of mutant GlaBs was shown as not being affected by mutation.

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Breeding of Aspergillus oryzae mutant strain with a high level of glucoamylase activity and non-melanization

Cited by 2 publications

References 0 publications

Copper ion (Cu2+) is involved in the transcription of the tyrosinase-encoding melB gene of Aspergillus oryzae in solid-state culture

Copper ion (Cu2+) is involved in the transcription of the tyrosinase-encoding melB gene of Aspergillus oryzae in solid-state culture

Comparison of Biochemical Properties of Wild Type Glucoamylases and Those of High Glucoamylase Mutant Strains of <i>Aspergillus oryzae</i>

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