Breeding value of dihaploid lines of spring rapeseed (Brassica napus L.)

Bochkaryova, E.B.; Горлова, Л.А.; Serdyuk, V.V.; Стрельников, Е А

doi:10.25230/2412-608x-2019-4-180-18-22

Cited by 2 publications

(1 citation statement)

References 1 publication

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…В опытах по оценке сортов и линий рапса в качестве родительских форм гибридов во ВНИИМК использовали озимые сорта и инбредные линии 5-7 поколения с высокой общей и специфической комбинационной способностью (Bochkaryova et al, 2019b). У ярового рапса изучали дигаплоидные линии, полученные методом культуры пыльников и изолированных микроспор на основе гибридов F 1 сортов селекции ВНИИМК 'Кубанский', 'Эввин', 'Ярвэлон' с сортами иностранной селекции (Bochkaryova et al, 2019b). Было установлено, что перевод линий рапса с фертильной цитоплазмы на цитоплазму Ogura не оказывает негативного влияния на такие хозяйственные признаки, как урожайность семян, масличность, продолжительность вегетационного периода, высота растения (Bochkaryova et al, 2018).…”

Section: системы цмс рапсаunclassified

CMS systems in rapeseed and their use in the breeding of domestic hybrids

Anisimova

Dubovskaya

2020

Trudy po prikladnoy botanike, genetike i selektsii

View full text Add to dashboard Cite

Development of heterotic hybrids is the most efficient approach to solve the problem of increasing the yield of rapeseed (Brassica napus L.), a leading oilseed crop. The cytoplasmic male sterility (CMS), widely used in rapeseed hybrid seed production, makes it possible to control hybridization between female and male lines. A review of publications on the nature of CMS systems in rapeseed and their utilization in breeding is presented. In rapeseed there are more than 10 known CMS systems of alloplasmic and homoplasmic origin. The male sterility character proved to be determined by chimeric mitochondrial genes, characterized by the presence of novel transcribed open reading frames (orf). Mitochondrial CMS genes associated with nap, pol, ogu and Nsa sterility types as well as nuclear Rf genes for pollen fertility restoration were identified. Molecular marker systems for identification of CMS-inducing and male fertility restoring genes were developed. The ogu, pol, MSL and inap CMS systems are commonly used for producing industrial rapeseed hybrids. The State Register of the Russian Federation for 2019 contains rapeseed hybrids of only foreign origin. Main achievements in domestic rapeseed hybrid production are highlighted. Research and breeding institutions developed new source material for rapeseed hete rotic hybrid breeding in various regions of the country. The sterility and fertility restoration sources were received from Canadian and French institutions as well as from domestic working collections. The yield structure traits did not deteriorate after transferring hybrid maternal lines to the sterile cytoplasm, while the glucosinolate content increased when pollen fertility restoring genes were transferred into paternal lines. Dihaploid (androclinium) lines and in vitro culture of unfertilized ovules were used to accelerate the breeding process. Experimental hybrids were developed using pol and ogu CMS.

show abstract

Section: системы цмс рапсаunclassified

CMS systems in rapeseed and their use in the breeding of domestic hybrids

Anisimova

Dubovskaya

2020

Trudy po prikladnoy botanike, genetike i selektsii

View full text Add to dashboard Cite

show abstract

Obtaining Haploids and Dihaploids of Sugar Beet (Beta Vulgaris L.) in Vitro Conditions

Kliachenko¹,

Prysiazhnuk²

2022

Biological Systems: Theory and Innovation

View full text Add to dashboard Cite

Biotechnological methods play a significant role in the breeding process, as they have a mechanism to develop parent material with high efficiency. The priority approach in modern innovative plant biotechnology is the method of experimental haploidy. The stepwise pretreatment of isolated buds with reduced (+4°C for 36 hours) and increased temperatures (+22°C for 21 days and +30°C – 10, 14, 21 days) was selected and applied, which ensures to complete the differentiation process. It was shown that for the induction of embryogenic development, regardless of the genotype, the optimal temperature was +30°C for the cultivation for 21 days. At the same time, the regenerative capacity of the obtained embryos depended on the initial genotype. The obtained rooted haploid regenerant plants with normal geo- and phototropic reaction were transferred to the diploid level (2n=38) by treatment the root system of an aqueous solution of 0.1% colchicine for 4 hours. It was found that the creation of homozygous lines depends on genotypic characteristics of the donor material, which defines the number of haploid regenerant plants obtained with various specific features. In this case, the sequence of nutrient media for the stabilization of haploid forms is strong important. Depending on the regenerants morphological development, the alternation of hormone-free and media supplemented with phytohormones contributes to the survival of plants in the range of 90-95%. The explants influence on the regenerative capacity of haploid plants was studied. It is shown that the nerves and leafstalks of sugar beet are optimal for inducing the processes of direct and indirect morphogenesis in vitro. The developed method for obtaining haploid plants directly from CMS lines of sugar beets makes it possible to obtain homozygous material in a short time. These materials can be used in heterosis breeding process, since homozygosity is achieved by traditional methods through inbreeding and the appearance of inbred depression.

show abstract

Breeding value of dihaploid lines of spring rapeseed (Brassica napus L.)

Abstract: Breeding value of dihaploid lines of spring rapeseed (Brassica napus L.). E.B. Bochkaryova, doctor of agriculture L.A. Gorlova, PhD in biology V.V. Serdyuk, senior researcher E.A. Strelnikov, PhD in biology

Cited by 2 publications

References 1 publication

CMS systems in rapeseed and their use in the breeding of domestic hybrids

CMS systems in rapeseed and their use in the breeding of domestic hybrids

Obtaining Haploids and Dihaploids of Sugar Beet (Beta Vulgaris L.) in Vitro Conditions

Contact Info

Product

Resources

About