Brevibacillus nitrificans sp. nov., a nitrifying bacterium isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks

Abstract

A heterotrophic nitrifying bacterium, designated strain DA2 T , was isolated from a microbiological agent for enhancing microbial digestion in sewage treatment tanks. Cells of strain DA2 T were Gram-positive, facultatively anaerobic, sporulating rods that were motile by means of peritrichous flagella; they were able to grow at pH 5-8. The major isoprenoid quinone of strain DA2 T was menaquinone-7 (MK-7) and its cellular fatty acid profile consisted mainly of iso-C 15 : 0 (18.6 %) and anteiso-C 15 : 0 (69.1 %). The DNA G+C content was 54.1 mol%. 16S rRNA gene sequence phylogeny suggested that strain DA2 T is a member of the genus Brevibacillus, with highest sequence similarities (in parentheses) to the type strains of Brevibacillus choshinensis (99.7 %), B. formosus (99.4 %), B. brevis (99.4 %), B. agri (99.0 %), B. reuszeri (98.8 %), B. parabrevis (98.7 %), B. centrosporus (98.6 %), B. limnophilus (97.4 %), B. panacihumi (97.3 %) and B. invocatus (97.3 %). DNA-DNA hybridization showed less than 60 % relatedness between strain DA2 T and type strains of the most closely related species given above. Given the significant differences in phenotypic and chemotaxonomic characteristics, and phylogenetic analysis based on the 16S rRNA sequence and DNA-DNA relatedness data, the isolate merits classification as a novel species, for which the name Brevibacillus nitrificans is proposed; the type strain of this species is DA2 T (5JCM 15774 T 5NCIMB 14531 T ).

Phenol-oxidizing activity and fatty acid profile of Brevibacillus centrosporus F14 strain

“…The fatty acid profile of Brevibacillus centrosporus F14 was similar to that of other Brevibacillus species, but differed from them, as were the strains of Brevibacillus limnophilus sp. nov. DSM 6472 (12.40%) [15], Brevibacillus invocatus JCM 12215T (7.9%) [16], with a high content of i16:0 fatty acid. For example, in the fatty acid profile of the strain Brevibacillus agri NBRC 15538T, the i-16:0 fatty acid was recorded in an amount up to 2% [16].…”

Non-contiguous finished genome sequence and description of Gorillibacterium massiliense gen. nov, sp. nov., a new member of the family Paenibacillaceae

“…When compared with representative species from the family Paenibacillaceae [38-42], G. massiliense gen. nov., sp.…”

Draft Genome Sequence of Brevibacillus sp. Strain BAB-2500, a Strain That Might Play an Important Role in Agriculture

“…He reclassified nine species of the genus Bacillus , i.e., Bacillus brevis (2), Bacillus laterosporus (3) Bacillus thermoruber (4), Bacillus agri (5), Bacillus centrosporus (5), Bacillus choshinensis (6), Bacillus borstelensis (7), Bacillus formosus (7), and Bacillus reuszeri (7), and placed them within a new genus, Brevibacillus (1). Subsequently, 8 validly named species were placed taxonomically within this genus: Brevibacillus invocatus (8), Brevibacillus limnophilus (9), Brevibacillus levickii (10), Brevibacillus ginsengisoli (11), Brevibacillus panacihumi (12), Brevibacillus fluminis (13), Brevibacillus aydinogluensis (14), and Brevibacillus nitrificans (15). Members of this genus are reported to be rod-shaped, Gram-positive or Gram-variable, motile by means of peritrichous flagella, and strictly aerobic, with G+C content ranging from 42.8 to 57.4%.…”