Brevilactibacter coleopterorum sp. nov., isolated from the intestine of the dark diving beetle Hydrophilus acuminatus, and Weissella coleopterorum sp. nov., isolated from the intestine of the diving beetle Cybister lewisianus

Abstract

A polyphasic taxonomic approach was used to characterize two novel bacterial strains, designated as HDW11T and HDW19T, isolated from intestine samples of the dark diving beetle Hydrophilus acuminatus and the diving beetle Cybister lewisianus, respectively. Both isolates were Gram-stain-positive, facultatively anaerobic and non-motile. Strain HDW11T grew optimally at 30 °C, pH 8 and in the presence of 1% (w/v) NaCl. Strain HDW19T grew optimally at 25 °C, pH 7 and in the presence of 0.3% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences and genome sequences revealed that strain HDW11T is a member of the genus Brevilactibacter and is closely related to Brevilactibacter flavus VG341T [with 97.9% 16S rRNA sequence identity and 79.1% average nucleotide identity (ANI)], and that strain HDW19T belongs to the genus Weissella and is closely related to W. koreensis KCTC 3621T (with 98.9% 16S rRNA sequence identity and 79.5% ANI). The major cellular fatty acids of strains HDW11T and HDW19T were C18:1 ω9c and anteiso-C15:0, respectively. The sole respiratory quinone of strain HDW11T was MK-9 (H4). The major polar lipid components of strain HDW11T were diphosphatidylglycerol and phosphatidylglycerol, and the major polar lipid component of strain HDW19T was diphosphatidylglycerol. The genomic DNA G+C content of strains HDW11T and HDW19T were 72.1 and 37.2 mol%, respectively. The results of phylogenetic, phenotypic, chemotaxonomic and genotypic analyses suggest that strain HDW11T represents a novel species within the genus Brevilactibacter , and that strain HDW19T represents a novel species within the genus Weissella . We propose the name Brevilactibacter coleopterorum sp. nov. for strain HDW11T (=KACC 21335T=KCTC 49320T=JCM 33680T) and the name Weissella coleopterorum for strain HDW19T (=KACC 21347T=KCTC 43114T=JCM 33684T).

Phylogenomic and comparative genomic analyses of Leuconostocaceae species: identification of molecular signatures specific for the genera Leuconostoc, Fructobacillus and Oenococcus and proposal for a novel genus Periweissella gen. nov.

“…The results presented here also provide compelling evidence that the members of the genus Weissella do not constitute a monophyletic grouping but instead comprise two distinct unrelated clades, designated in this work as the 'Weissella main clade' and 'Weissella clade 2' . The branching of Weissella species into two distantly related clades is also observed in earlier studies on the members of this genus [24][25][26][27][28][29]. In the present work, we have identified six and five CSIs that exclusively found in either different species from the Weissella main clade, or which are specific for the Weissella clade 2 species.…”

“…In the published 16S rRNA gene trees, the genera Leuconostoc , Fructobacillus and Convivina form a strongly supported clade [7, 8]. Additionally, in most of the phylogenetic studies of Weissella species [24–30], several species from this genus ( viz . Weissella beninensis , Weissella fabalis , Weissella fabaria , Weissella ghanensis and Weissella cryptocerci ) branch distinctly from the main clade of Weissella species containing the type species ( Weissella viridescens ) of this genus [12].…”

Novel Insights Into the Phylogeny and Biotechnological Potential of Weissella Species

“…Our data partially validate the taxonomic clustering previously proposed by De Bruyne et al ( 2010) and Fusco et al (2015), which were essentially based on the 16S rRNA gene and pheS gene sequence phylogenies, with the latter showing a higher discriminatory power as a marker gene. The description of novel species (Lee et al, 2015;Praet et al, 2015;Heo et al, 2019;Li et al, 2020;Lin et al, 2020;Hyun et al, 2021; this study) and the availability of additional genomic sequences (refer to Supplementary Table S1) allowed us to perform a comprehensive genomic-based phylogenetic analysis of all species in the genus Weissella. Our analysis also integrates genomic indexes, which are currently considered as minimal standards to perform taxonomic classification.…”

“…The second species group, designated as the W. kandleri group, comprises W. kandleri, W. diestrammenae, W. koreensis, W. soli, and W. coleoptorum. The latter was described in 2021 (Hyun et al, 2021) and was therefore not represented in the previous classification. The 16S rRNA gene sequence identity among these species is 97.1% on average, while the ANI value is 82%, with W. coleoptorum and W. soli being the closest related, sharing an ANI value of 83.91%.…”

“…Since 2015, seven additional Weissella species have been described, including W. bombi (Praet et al, 2015 ), W. coleopterorum (Hyun et al, 2021 ), W. cryptocerci (Heo et al, 2019 ), W. muntiaci (Lin et al, 2020 ), W. sagaensis (Li et al, 2020 ), and W. jogaejeotgali (Lee et al, 2015 ). According to Kwak et al ( 2019 ), W. jogaejeotgali is a later heterotypic synonym of W. thailandensis , while Ennahar and Cai ( 2004 ) reported that W. kimchii (Choi et al, 2002 ) is a later heterotypic synonym of W. cibaria (Björkroth et al, 2002 ).…”