Brewer’s spent grain as a no-cost substrate for polyhydroxyalkanoates production: Assessment of pretreatment strategies and different bacterial strains

Corchado-Lopo, Carlos; Martínez-Avila, Oscar; Marti, Elisabet; Llimós, Jordi; Busquets, Anna María; Kučera, Dan; Obruča, Stanislav; Llenas, Laia; Ponsá, Sergio

doi:10.1016/j.nbt.2021.01.009

Cited by 27 publications

(19 citation statements)

References 46 publications

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“…Similar results have also been reported in earlier literatures [38,39]. High yield observed with utilization of brewer's spent grain hydrolysate could be a function of its various composition and pretreatment method used.…”

Section: Discussionsupporting

confidence: 91%

Molecular Characterization, Optimization and Production of PHA by Indigenous Bacteria Using Alternative Nutrient Sources as Substrate

Dienye

Agwa

Abu

2022

MRJI

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Polyhydroxyalkanoates (PHA) are renewable, biodegradable biopolymer intracellularly accumulated by wide array of microorganisms as carbon reserve. This study investigates the influence of various cultural conditions on PHA production by a recently isolated local species under submerged fermentation. Six PHA producing strains were identified by 16S rDNA gene sequencing and strain Priestia flexa OWO1 showed satisfactory PHA productivity. The effects of production parameters were investigated and extraction of PHA was carried out using sodium hypochlorite method and maximum amount was detected after 72h. Maximum PHA production was obtained at incubation period of 48h, pH of 7.0 and temperature of 30oC. Amongst the hydrolysate of agro waste used, brewers spent grain (BSG) gave maximum yield of 3.01g/L while beans bran powder gave the highest PHA yields of 3.9 g/L amongst the organic nitrogen sources tested. Analysis of the crude PHA by Fourier Transform Infrared Spectroscopy (FT-IR) showed the presence of methyl, methylene as well as carbonyl functional groups. PHA production was higher after optimizing the production conditions as compared to basal medium therefore the utilization of these cheap renewable resources as alternative substrates for production of PHA make the process cost effective and sustainable.

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Section: Discussionsupporting

confidence: 91%

Molecular Characterization, Optimization and Production of PHA by Indigenous Bacteria Using Alternative Nutrient Sources as Substrate

Dienye

Agwa

Abu

2022

MRJI

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“…It was provided that the spent coffee grounds are rich in fatty acids, amino acids, lignin, polysaccahrides, polyphenols, tannins, and flavonoids, thus making this feedstock a potential substate for biofuel production, adsorbents, and chemical production [18][19][20]. There are some examples of investigations on the treatment and application of spent coffee grounds: (i) valorisation of SCG in solid biomass fuel in the form of briquettes using xanthan gum under low temperature and low pressure conditions [21]; (ii) gasification of SCG which gave good results and demonstrated the high energy potential of this material [22]; (iii) polyhydroxyalkanoates (chemical industry) [23]. The brewery industry generates spent grains, which are 85% of the total waste generated by brewery.…”

Section: Introductionmentioning

confidence: 99%

Pyrolysis of Biomass Wastes into Carbon Materials

et al. 2022

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This study presents the results of the biomass pyrolysis process focusing on biochar production and its potential energetic (as solid fuel) and material (as adsorbent) applications. Three kinds of biomass waste were investigated: wheat straw, spent coffee grounds, and brewery grains. The pyrolysis process was carried out under nitrogen atmosphere at 400 and 500 °C (residence time of 20 min). A significant increase in the carbon content was observed in the biochars, e.g., from 45% to 73% (at 400 °C) and 77% (at 500 °C) for spent coffee grounds. In addition, the structure and morphology were investigated using scanning electron microscopy. Thermal properties were studied using a simultaneous thermal analysis under an oxidising atmosphere. The chemical activation was completed using KOH. The sorption properties of the obtained biochars were tested using chromium ion (Cr3+) adsorption from liquid solution. The specific surface area and average pore diameter of each sample were determined using the BET method. Finally, it was found that selected biochars can be applied as adsorbent or a fuel. In detail, brewery grains-activated carbon had the highest surface area, wheat straw-activated carbon adsorbed the highest amount of Cr3+, and wheat straw chars presented the best combustion properties.

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“…Nevertheless, because of its composition, BSG is a potential feedstock for several applications, namely for polymers production [16]. Currently, BSG has been used to produce PHAs using single strains (Burkholderia cepacia, Bacillus cereus, and Cupriavidus necator), achieving a PHA yield of up to 23 mg PHA/g BSG using B. cepacia, showing the potential application of BSG as a feedstock for added-value product production [17][18][19][20]. The PHA production by MMC can be more economically advantageous over pure/single culture production; however, to the best of our knowledge, this has not yet been reported.…”

Section: Introductionmentioning

confidence: 99%

“…The composition of BSG (lignocellulosic and recalcitrant materials content), as well as the solid-state form of this raw waste, present a challenge for its use in biological processes, as it is usually necessary for a pre-treatment to increase the process performance. However, this challenge can be overcome when MMCs are used, as the BSG can be fermented in the solid state, producing a VFA-rich stream [12], and then fed to MMC for PHA production, presenting an advantage over single cultures that usually use the BSG hydrolyzed or supplemented with other carbon sources to increase the PHA productivity [17][18][19][20]. Moreover, the use of fermented BSG by MMC allows for producing PHA with different compositions, depending on the VFA profile, which represents an advantage for PHA application.…”

Section: Introductionmentioning

confidence: 99%

Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome

Carvalheira

Amorim

Oliveira

et al. 2022

Life

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Raw brewers’ spent grain (BSG), a by-product of beer production and produced at a large scale, presents a composition that has been shown to have potential as feedstock for several biological processes, such as polyhydroxyalkanoates (PHAs) production. Although the high interest in the PHA production from waste, the bioconversion of BSG into PHA using microbial mixed cultures (MMC) has not yet been explored. This study explored the feasibility to produce PHA from BSG through the enrichment of a mixed microbial culture in PHA-storing organisms. The increase in organic loading rate (OLR) was shown to have only a slight influence on the process performance, although a high selectivity in PHA-storing microorganisms accumulation was reached. The culture was enriched on various PHA-storing microorganisms, such as bacteria belonging to the Meganema, Carnobacterium, Leucobacter, and Paracocccus genera. The enrichment process led to specialization of the microbiome, but the high diversity in PHA-storing microorganisms could have contributed to the process stability and efficiency, allowing for achieving a maximum PHA content of 35.2 ± 5.5 wt.% (VSS basis) and a yield of 0.61 ± 0.09 CmmolPHA/CmmolVFA in the accumulation assays. Overall, the production of PHA from fermented BSG is a feasible process confirming the valorization potential of the feedstock through the production of added-value products.

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Brewer’s spent grain as a no-cost substrate for polyhydroxyalkanoates production: Assessment of pretreatment strategies and different bacterial strains

Cited by 27 publications

References 46 publications

Molecular Characterization, Optimization and Production of PHA by Indigenous Bacteria Using Alternative Nutrient Sources as Substrate

Molecular Characterization, Optimization and Production of PHA by Indigenous Bacteria Using Alternative Nutrient Sources as Substrate

Pyrolysis of Biomass Wastes into Carbon Materials

Valorization of Brewery Waste through Polyhydroxyalkanoates Production Supported by a Metabolic Specialized Microbiome

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