Bridging the gap—Biocompatibility of microelectronic materials

Bogner, Elisabeth; Dominizi, K.; Hagl, P.; Bertagnolli, E.; Wirth, Michael; Gabor, Franz; Brezna, W.; Wanzenboeck, Heinz D.

doi:10.1016/j.actbio.2005.10.006

Cited by 23 publications

(21 citation statements)

References 17 publications

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“…[9] We used silicon and photoresist SU-8 (MicroChem) as structured substrates that were coated with 100 nm titanium. The biocompatibility of these materials was investigated in Refs [10,11]. Arrays of equidistant quadratic photo resist pillars with vertical side walls were obtained by a photolithographic process on Si-wafers: For good adhesion of the photo resist to the substrate the wafers were cleaned in an oxygen plasma followed by an ammonium fluoride dip to remove the natural oxide layer before coating them with a 5-µm-thick layer of the commonly used negative photo resist SU-8.…”

Section: Methodsmentioning

confidence: 99%

Titanium surfaces structured with regular geometry—material investigations and cell morphology

Lange

Elter

Biała

et al. 2010

Surface & Interface Analysis

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Mathematical modeling of the cell-material contact demands a thorough characterization of both the material surface and the cellular reaction. In earlier investigations we used stochastic material surfaces for this purpose. Also, other groups working on the same or similar subject used such stochastically structured material surfaces. In continuation of this work we now use fine-structured material surfaces with well-defined regular geometry because a stepwise and systematic variation of the structural parameters of geometrically defined surfaces makes it easier to find the characteristic parameters for modeling the cell-material interface. To begin with, we used grooves with rectangular profiles and cubic pillars. The grooves were etched in silicon by dry etching and then were sputter coated with 100 nm titanium. Arrays of cubic photo resist pillars with vertical side walls in different dimensions were obtained by a photolithographic process and were also sputter coated with 100 nm titanium. The samples were characterized by SEM and electrochemical methods. Human osteoblastic cells MG-63 (ATCC, LGC promochem, Manassas, USA) were cultured in Dulbecco's modified eagle medium with 10% fetal calf serum (FCS) at 37• C and 5% CO 2 . MG-63 cells grown on microstructures within 24 h were visualized by SEM. We observed that cells on the grooved surfaces grew along the grooves mainly adhering to the horizontal edges of the material surface, whereas on the pillar structures the cells lie on the top of the pillars and are well spread. But the direction of spreading on the pillars is also determined by the horizontal and vertical edges of the surface structure.

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Section: Methodsmentioning

confidence: 99%

Titanium surfaces structured with regular geometry—material investigations and cell morphology

Lange

Elter

Biała

et al. 2010

Surface & Interface Analysis

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“…The attachment and growth characteristics of cells differ for different cell types cultured in similar environments. Our future plans are therefore also focused on performing viability experiments (adhesion and proliferation) with different adherent cell types, and determine the effect of the surface chemistry on the adhesion properties and growth rates, as also performed by Bogner et al for human adenocarcinoma cells [32]. In conclusion, this study has demonstrated that when performing cell analyses with HL60 cells in lab-on-a-chip devices, the choice of material plays a minor role.…”

Section: Pimentioning

confidence: 86%

“…Moreover, the results obtained upon analyzing the viability using Annexin V in combination with PI and the cell cycle measurements demonstrated that the microchip materials used, irrespective of their surface chemistry, are all suitable for HL60 cells. The presented assays here all measure different parameters having an effect on the viability, which might be a reason why the beneficial results [31,32]. Our hypothesis is that the effect of microchip materials is more pronounced when using adherent cells.…”

Section: Pimentioning

confidence: 90%

Viability study of HL60 cells in contact with commonly used microchip materials

et al. 2006

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This paper presents a study in which different commonly used microchip materials (silicon oxide, borosilicate glass, and PDMS) were analyzed for their effect on human promyelocytic leukemic (HL60) cells. Copper-coated silicon was analyzed for its toxicity and therefore served as a positive control. With quantitative PCR, the expression of the proliferation marker Cyclin D1 and the apoptosis marker tissue transglutaminase were measured. Flow cytometry was used to analyze the distribution through the different phases of the cell cycle (propidium iodide, PI) and the apoptotic cascade (Annexin V in combination with PI). All microchip materials, with the exception of Cu, appeared to be suitable for HL60 cells, showing a ratio apoptosis/proliferation (R(ap)) comparable to materials used in conventional cell culture (polystyrene). These results were confirmed with cell cycle analysis and apoptosis studies. Precoating the microchip material surfaces with serum favor the proliferation, as demonstrated by a lower R(ap) as compared to uncoated surfaces. The Cu-coated surface appeared to be toxic for HL60 cells, showing over 90% decreased viability within 24 h. From these results, it can be concluded that the chosen protocol is suitable for selection of the cell culture material, and that the most commonly used microchip materials are compatible with HL60 culturing.

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“…The biocompatibility of ITO for long-term implantation is not well understood. Recent studies focusing on cell adhesion and cell proliferation [25] as well as protein adsorption [26] have shown ITO to be biocompatible. Titanium (Ti) has excellent tissue compatibility and it has the ability to form an oxide layer on the surface, which makes it even more susceptible to corrosion [23,27,28], and has been shown to exhibit properties that are favorable for blood contact [29].…”

Section: Implantable Bioelectronic Device Materialsmentioning

confidence: 99%

Improving the Biocompatibility of Implantable Bioelectronics Devices

Slaughter

2014

Implantable Bioelectronics

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Bridging the gap—Biocompatibility of microelectronic materials

Cited by 23 publications

References 17 publications

Titanium surfaces structured with regular geometry—material investigations and cell morphology

Titanium surfaces structured with regular geometry—material investigations and cell morphology

Viability study of HL60 cells in contact with commonly used microchip materials

Improving the Biocompatibility of Implantable Bioelectronics Devices

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