Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish

Blechinger, Scott R.; Kusch, Robin C.; K, Haugo; Matz, Carlyn J; Chivers, Douglas P.; Krone, Patrick H.

doi:10.1016/j.taap.2007.06.025

Cited by 92 publications

(62 citation statements)

References 43 publications

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“…This protein has been reported to be induced by a variety of heavy metals including Zn, Cd, Hg, Ag and Cu (Murata et al, 1999;Williams et al, 1990) and this activation is mediated by the heat shock transcription factor HSF-1 (Mosser et al, 1988;Murata et al, 1999). Recent work has shown that copper can activate the human hsp70 promoter in mosaic transgenic fi sh (Seok et al, 2006), and previous reports show that heat shock and cadmium induce the expression of EGFP in Tg(hsp70:egfp) fi sh expressing EGFP driven by the hsp70 gene promoter (Blechinger et al, 2002a;Blechinger et al, 2002b;Blechinger et al, 2007;Matz and Krone, 2007). Using these transgenic fish, we found that waterborne copper induces EGFP expression in a concentration-dependent manner.…”

Section: Discussionmentioning

confidence: 99%

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

et al. 2011

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Copper is an essential ion that forms part of the active sites of many proteins. At the same time, an excess of this metal produces free radicals that are toxic for cells and organisms. Fish have been used extensively to study the effects of metals, including copper, present in food or the environment. It has been shown that different metals induce different adaptive responses in adult fi sh. However, until now, scant information has been available about the responses that are induced by waterborne copper during early life stages of fi sh. Here, acute toxicity tests and LC50 curves have been generated for zebrafi sh larvae exposed to dissolved copper sulphate at different concentrations and for different treatment times. We determined that the larvae incorporate and accumulate copper present in the medium in a concentrationdependent manner, resulting in changes in gene expression. Using a transgenic fi sh line that expresses enhanced green fl uorescent protein (EGFP) under the hsp70 promoter, we monitored tissue-specifi c stress responses to waterborne copper by following expression of the reporter. Furthermore, TUNEL assays revealed which tissues are more susceptible to cell death after exposure to copper. Our results establish a framework for the analysis of whole-organism management of excess external copper in developing aquatic animals.

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Section: Discussionmentioning

confidence: 99%

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

et al. 2011

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“…However, very little else has been revealed about the chemistry of these cues. Waterborne cadmium exposures at environmentally relevant levels have been shown to result in the deposition of cadmium within the olfactory sensory neurons of the fish's olfactory system (olfactory rosette, nerve, and bulb) [20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…Carreau and Pyle [26] provide supportive evidence for this hypothesis, as they showed that fathead minnows (P. promelas) reared under 10 g Cu/L until hatching did not respond to alarm cues even after an 84-d depuration period. Furthermore, Blechinger et al [22] showed that 3-h-pulseexposed hsp70/eGFP transgenic zebrafish (Danio rerio) to 14.1 mg Cd/L exhibited a reduction in response to alarm cues even after a four-to six-week depuration period. Studies have demonstrated that olfaction can be temporarily altered by various pollutants that accumulate in the olfactory system, such as cadmium [21][22][23]27], copper [26], and humic acid [28].…”

Section: Introductionmentioning

confidence: 99%

“…Furthermore, Blechinger et al [22] showed that 3-h-pulseexposed hsp70/eGFP transgenic zebrafish (Danio rerio) to 14.1 mg Cd/L exhibited a reduction in response to alarm cues even after a four-to six-week depuration period. Studies have demonstrated that olfaction can be temporarily altered by various pollutants that accumulate in the olfactory system, such as cadmium [21][22][23]27], copper [26], and humic acid [28]. Moreover, the studies allude to the possibility of olfactory disruption affecting various aspects of fitness.…”

Section: Introductionmentioning

confidence: 99%

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Chronic exposure to low concentrations of waterborne cadmium during embryonic and larval development results in the long‐term hindrance of antipredator behavior in zebrafish

Kusch

Krone

Chivers

2008

Enviro Toxic and Chemistry

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Abstract-Cadmium has been recognized for some time as a potent environmental pollutant with the capability of disrupting olfactory-mediated behaviors. Failing to respond to chemical cues in the environment could adversely affect foraging, reproduction and predator avoidance. Recognizing this impaired perception as a serious ecological problem has been undermined by the fact that the damage is often reversible; short depuration periods of 5 d may allow for the re-establishment of responses to chemical cues. In this experiment, early life stage zebrafish were continuously exposed for 50 d at 0, 0.2, 2.0, and 20 g Cd/L. The subjects were depurated for 14 d and then subjected to behavioral testing where antipredator responses to chemical alarm cues were observed. Our data show that continuous exposure during rearing to a concentration as low as 20 g Cd/L is sufficient at eliminating antipredator behavior in zebrafish (Danio rerio) even after the source of the cadmium had been removed for 14 d. Furthermore, subjects raised under a 10-fold lower concentration also showed alteration in their behavioral responses, taking significantly longer to respond to the predation threat. Exposure to low levels of cadmium throughout development may alter neurogenesis, subsequently resulting in long-term impairment of chemical cue perception.

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“…SP10 Aquatic Ecology & Toxicology Section, Centre for Organismal Studies (COS), University of Heidelberg (Daniel Stengel, Thomas Braunbeck). They are responsible for the development of a neurotoxicological test battery with zebra fish (D. rerio) using three sensory systems (olfaction [28], vision [29], and lateral line [30]), one synaptic system (cholinergic transmission), and a major physiological end point (embryo toxicity). State-of -the-art imaging techniques and an enzyme assay are used to detect neurotoxic effects in vivo [31][32][33].…”

Section: Module Neurotoxicitymentioning

confidence: 99%

Tox-Box: securing drops of life - an enhanced health-related approach for risk assessment of drinking water in Germany

et al. 2013

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This article introduces 'Tox-Box', a joint research project designed to develop a holistic approach towards a harmonized testing strategy for exposure-and hazard-based risk management of anthropogenic trace substances in drinking water to secure a long-term drinking water supply. The main task of the Tox-Box consortium is to enhance the existing health-related indicator value concept (German: GOW-Konzept -Gesundheitlicher Orientierungswert) through development and prioritization of additional end point-related testing strategies for genotoxicity, neurotoxicity, germ cell damage, and endocrine effects. In this context, substance-specific modes of action will be identified and characterized. Toxicological data collected by the 12 Tox-Box subprojects will be evaluated and weighted to structure a hierarchical testing strategy for an improved risk assessment. A technical guidance document for exposure and hazard-based risk management of anthropogenic trace substances in drinking water will eventually be prepared.

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Brief embryonic cadmium exposure induces a stress response and cell death in the developing olfactory system followed by long-term olfactory deficits in juvenile zebrafish

Cited by 92 publications

References 43 publications

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

Sublethal concentrations of waterborne copper induce cellular stress and cell death in zebrafish embryos and larvae

Chronic exposure to low concentrations of waterborne cadmium during embryonic and larval development results in the long‐term hindrance of antipredator behavior in zebrafish

Tox-Box: securing drops of life - an enhanced health-related approach for risk assessment of drinking water in Germany

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