Bright Polymer Dots Tracking Stem Cell Engraftment and Migration to Injured Mouse Liver

Chen, Dandan; Li, Qiong; Meng, Zihui; Guo, Lei; Tang, Ying; Liu, Zhihe; Yin, Shengyan; Qin, Weiping; Yuan, Zhen; Zhang, Xuanjun; Wu, Changfeng

doi:10.7150/thno.18614

Cited by 50 publications

(46 citation statements)

References 71 publications

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“…Also, the polymer dot-labeled MSCs retained robust self-renewal capacity and multi-lineage differentiation potential. In another study, researchers found that, compared to unlabeled MSCs, cell viability, proliferation and differentiation capacity, tumorigenicity, and immunophenotypic profiles of polymer dot-labeled MSCs also showed no differences 120…”

Section: Nps and Their Toxic Effectsmentioning

confidence: 97%

<p>A brief review of cytotoxicity of nanoparticles on mesenchymal stem cells in regenerative medicine</p>

Yang

Sun

et al. 2019

IJN

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Multipotent mesenchymal stem cells have shown great promise for application in regenerative medicine owing to their particular therapeutic effects, such as significant self-renewability, low immunogenicity, and ability to differentiate into a variety of specialized cells. However, there remain certain complicated and unavoidable problems that limit their further development and application. One of the challenges is to noninvasively monitor the delivery and biodistribution of transplanted stem cells during treatment without relying on behavioral endpoints or tissue histology, and it is important to explore the potential mechanisms to clarify how stem cells work in vivo. To solve these problems, various nanoparticles (NPs) and their corresponding imaging methods have been developed recently and have made great progress. In this review, we mainly discuss NPs used to label stem cells and their toxic effects on the latter, the imaging techniques to detect such NPs, and the current existing challenges in this field.

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Section: Nps and Their Toxic Effectsmentioning

confidence: 97%

<p>A brief review of cytotoxicity of nanoparticles on mesenchymal stem cells in regenerative medicine</p>

Yang

Sun

et al. 2019

IJN

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“…CPPs can enter cells without causing cytotoxicity at the working does [ 22 ]; therefore, they have been used as a tool for the delivery of various cargoes [ 48 – 50 ]. Especially in stem cell tracking experiments, CPPs-modified imaging particles did not affect the differentiation potential of stem cells in vitro [ 27 , 51 , 52 ].…”

Section: Advantages Of Cpps In Stem Cell Trackingmentioning

confidence: 99%

Prospect of cell penetrating peptides in stem cell tracking

Zhang

Tong

2021

Stem Cell Res Ther

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Stem cell therapy has shown great efficacy in many diseases. However, the treatment mechanism is still unclear, which is a big obstacle for promoting clinical research. Therefore, it is particularly important to track transplanted stem cells in vivo, find out the distribution and condition of the stem cells, and furthermore reveal the treatment mechanism. Many tracking methods have been developed, including magnetic resonance imaging (MRI), fluorescence imaging, and ultrasound imaging (UI). Among them, MRI and UI techniques have been used in clinical. In stem cell tracking, a major drawback of these technologies is that the imaging signal is not strong enough, mainly due to the low cell penetration efficiency of imaging particles. Cell penetrating peptides (CPPs) have been widely used for cargo delivery due to its high efficacy, good safety properties, and wide delivery of various cargoes. However, there are few reports on the application of CPPs in current stem cell tracking methods. In this review, we systematically introduced the mechanism of CPPs into cell membranes and their advantages in stem cell tracking, discussed the clinical applications and limitations of CPPs, and finally we summarized several commonly used CPPs and their specific applications in stem cell tracking. Although it is not an innovation of tracer materials, CPPs as a powerful tool have broad prospects in stem cell tracking. Graphic abstract

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“…Although MSCs may form strategic niches in perivascular spaces in almost every region of the body, they migrate to sites of injury for inflammation suppression and wound healing after the detection of local and distant tissue damage [68, 69]. However, the injection of MSCs through the blood vessels leads to the engraftment of MSCs into the lungs and the capillary beds of other tissues or organs, consequently decreasing the number of MSCs migrating to the target areas [70]. RSV pretreatment in a common bile duct ligation (CBDL) animal model improved the therapeutic effects of MSCs via the upregulation of SIRT1 and the downregulation of p53, accompanied by the upregulation of the homing of MSCs to the liver and a decrease in homing of MSCs to the lung and spleen [62].…”

Section: The Effects Of Rsv On Mscs In Vivomentioning

confidence: 99%

The application of resveratrol to mesenchymal stromal cell-based regenerative medicine

Hu¹

2019

Stem Cell Res Ther

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Currently, the transplantation of mesenchymal stromal cells (MSCs) has emerged as an effective strategy to protect against tissue and organ injury. MSC transplantation also serves as a promising therapy for regenerative medicine, while poor engraftment and limited survival rates are major obstacles for its clinical application. Although multiple studies have focused on investigating chemicals to improve MSC stemness and differentiation in vitro and in vivo, there is still a shortage of effective and safe agents for MSC-based regenerative medicine. Resveratrol (RSV), a nonflavonoid polyphenol phytoalexin with a stilbene structure, was first identified in the root extract of white hellebore and is also found in the roots of Polygonum cuspidatum, and it is widely used in traditional Chinese medicine. RSV is a natural agent that possesses great therapeutic potential for protecting against acute or chronic injury in multiple tissues as a result of its antioxidative, anti-inflammatory, and anti-cancer properties. According to its demonstrated properties, RSV may improve the therapeutic effects of MSCs via enhancing their survival, self-renewal, lineage commitment, and anti-aging effects. In this review, we concluded that RSV significantly improved the preventive and therapeutic effects of MSCs against multiple diseases. We also described the underlying mechanisms of the effects of RSV on the survival, self-renewal, and lineage commitment of MSCs in vitro and in vivo. Upon further clarification of the potential mechanisms of the effects of RSV on MSC-based therapy, MSCs may be able to be more widely used in regenerative medicine to promote recovery from tissue injury.

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Bright Polymer Dots Tracking Stem Cell Engraftment and Migration to Injured Mouse Liver

Cited by 50 publications

References 71 publications

<p>A brief review of cytotoxicity of nanoparticles on mesenchymal stem cells in regenerative medicine</p>

<p>A brief review of cytotoxicity of nanoparticles on mesenchymal stem cells in regenerative medicine</p>

Prospect of cell penetrating peptides in stem cell tracking

The application of resveratrol to mesenchymal stromal cell-based regenerative medicine

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