Broad-Range PCR in Selected Episodes of Prosthetic Joint Infection

Man, F. H. R. De; Gräber, Peter; Lüem, M.; Zimmerli, Werner; Ochsner, P. E.; Sendi, Parham

doi:10.1007/s15010-008-8246-1

Cited by 62 publications

(45 citation statements)

References 13 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Broad-range 16S rRNA gene PCR analysis has already been evaluated for the diagnosis of PJI; 16S rRNA gene PCR assays of periprosthetic tissue or periprosthetic sonicate fluid samples showed a wide range of sensitivity and specificity values, from 50 to 92% and from 65 to 94%, respectively (9)(10)(11)(12)(13)(14)(15). Compared with conventional culture, the sensitivity of 16S rRNA gene PCR analysis was higher, lower, or equivalent, sometimes to the detriment of specificity (9)(10)(11)(12)(13)(14)(15).…”

mentioning

confidence: 99%

See 1 more Smart Citation

Evaluation of 16S rRNA Gene PCR Sensitivity and Specificity for Diagnosis of Prosthetic Joint Infection: a Prospective Multicenter Cross-Sectional Study

Bémer¹,

et al. 2014

View full text Add to dashboard Cite

There is no standard method for the diagnosis of prosthetic joint infection (PJI). The contribution of 16S rRNA gene PCR sequencing on a routine basis remains to be defined. We performed a prospective multicenter study to assess the contributions of 16S rRNA gene assays in PJI diagnosis. Over a 2-year period, all patients suspected to have PJIs and a few uninfected patients undergoing primary arthroplasty (control group) were included.

show abstract

mentioning

confidence: 99%

“…Compared with conventional culture, the sensitivity of 16S rRNA gene PCR analysis was higher, lower, or equivalent, sometimes to the detriment of specificity (9)(10)(11)(12)(13)(14)(15). More-recent studies on 16S rRNA gene PCR assays of periprosthetic sonicate fluids have also shown contradictory results.…”

mentioning

confidence: 99%

Evaluation of 16S rRNA Gene PCR Sensitivity and Specificity for Diagnosis of Prosthetic Joint Infection: a Prospective Multicenter Cross-Sectional Study

Bémer¹,

et al. 2014

View full text Add to dashboard Cite

show abstract

“…Molecular biology provided a diagnostic tool that showed its effectiveness, especially when antibiotics were administered before surgery or when fastidious bacteria were involved, particularly for the diagnosis of endocarditis (4). For BJI diagnosis, broad-range 16S rRNA gene PCR has shown higher, lower, or equivalent sensitivity compared with conventional culture methods, but sometimes to the detriment of specificity (5)(6)(7)(8)(9)(10)(11)(12)(13)(14)(15).…”

mentioning

confidence: 99%

First Experience of a Multicenter External Quality Assessment of Molecular 16S rRNA Gene Detection in Bone and Joint Infections

Plouzeau

Bémer²,

Valentin

et al. 2015

J Clin Microbiol

View full text Add to dashboard Cite

The objective of this study was to assess the performance of seven French laboratories for 16S rRNA gene detection by real-time PCR in the diagnosis of bone and joint infection (BJI) to validate a large multicenter study. External quality control (QC) was required owing to the differences in extraction procedures and the molecular equipment used in the different laboratories. Three proficiency sets were organized, including four bacterial DNA extracts and four bead mill-pretreated osteoarticular specimens. Extraction volumes, 16S rRNA gene primers, and sequencing interpretation rules were standardized. In order to assess each laboratory's ability to achieve the best results, scores were assigned, and each QC series was classified as optimal, acceptable, or to be improved. A total of 168 QCs were sent, and 160 responses were analyzed. The expected results were obtained for 93.8%, with the same proportion for extracts (75/80) and clinical specimens (75/80). For the specimens, there was no significant difference between manual and automated extraction. This QC demonstrated the ability to achieve good and homogeneous results using the same 16S rRNA gene PCR with different equipment and validates the possibility of high-quality multicenter studies using molecular diagnosis for BJI.

show abstract

“…Microbiologic diagnosis of PJI has traditionally been made by culture of synovial fluid, periprosthetic tissue, and/or the implant itself. However, cultures are not universally positive (1)(2)(3)(4)(5)(6); this has spawned an interest in using molecular strategies to diagnose PJI (7)(8)(9)(10)(11)(12)(13). We have recently shown that a technique that couples PCR with electrospray ionization mass spectrometry (PCR-ESI/MS), used previously in a variety of settings (14)(15)(16)(17)(18), can be applied to materials dislodged from explanted orthopedic implants (sonicate fluid) to diagnose PJI with increased sensitivity compared with culture (19).…”

mentioning

confidence: 99%

Detection of Prosthetic Joint Infection by Use of PCR-Electrospray Ionization Mass Spectrometry Applied to Synovial Fluid

et al. 2014

View full text Add to dashboard Cite

dPCR coupled with electrospray ionization mass spectrometry applied to synovial fluid specimens had an 81% sensitivity and a 95% specificity for the diagnosis of prosthetic joint infection.T he number of cases of prosthetic joint infection (PJI) is increasing. Microbiologic diagnosis of PJI has traditionally been made by culture of synovial fluid, periprosthetic tissue, and/or the implant itself. However, cultures are not universally positive (1-6); this has spawned an interest in using molecular strategies to diagnose PJI (7-13). We have recently shown that a technique that couples PCR with electrospray ionization mass spectrometry (PCR-ESI/MS), used previously in a variety of settings (14-18), can be applied to materials dislodged from explanted orthopedic implants (sonicate fluid) to diagnose PJI with increased sensitivity compared with culture (19). Jacovides et al. used an older version of this technology than we used and detected organisms in synovial fluid in 88% of presumed noninfectious arthroplasty failures (20). Although we also found a lower specificity of PCR-ESI/MS (94%) than culture (99%) when applied to sonicate fluid (21), we did not find nearly the proportion of positive specimens in presumed noninfectious failure reported by Jacovides et al. (20). Herein, we evaluated synovial fluid specimens collected in containers treated to minimize background DNA using the same version of the PCR-ESI/MS assay we used to study sonicate fluid (21).(This study was presented in part at the 113th General Meeting of the American Society for Microbiology, 18 to 21 May 2013, Denver, CO.)The PCR-ESI/MS BAC protocol (Ibis Biosciences, Carlsbad, CA) was used to test synovial fluid collected by sterile arthrocentesis from subjects with knee arthroplasty failure at the Mayo Clinic, Rochester, MN. The research application PCR-ESI/MS BAC assay studied detects more than 3,400 species of bacteria, 40 species of Candida, and four antibiotic resistance markers, bla KPC , vanA, vanB, and mecA. Syringes, Vacutainer collection tubes, and freezer storage vials used for collection and storage of specimens were pretreated to minimize contaminating DNA by irradiation in a self-contained 137 Cs gamma irradiator with a total dose of 1 Gy (22). Specimens were collected between 2001 and 2012 and stored at Ϫ70°C until PCR-ESI/MS testing in 2012. At the time of specimen collection, cultures had been performed using previously described methods (23). Subjects were classified as having PJI or aseptic failure (AF) using the 2011 Musculoskeletal Infection Society (MSIS) criteria, a combined clinical/laboratory classification system (24). Accordingly, a subject was considered to have PJI under the following conditions: if a sinus tract communicating with the prosthesis was found; if the same organism was isolated from two non-synovial fluid samples (i.e., periprosthetic tissue, sonicate fluid) obtained from the affected joint at the time of revision surgery; or if four of the following six minor criteria were present: elevated erythrocyte sed...

show abstract

Broad-Range PCR in Selected Episodes of Prosthetic Joint Infection

Cited by 62 publications

References 13 publications

Evaluation of 16S rRNA Gene PCR Sensitivity and Specificity for Diagnosis of Prosthetic Joint Infection: a Prospective Multicenter Cross-Sectional Study

Evaluation of 16S rRNA Gene PCR Sensitivity and Specificity for Diagnosis of Prosthetic Joint Infection: a Prospective Multicenter Cross-Sectional Study

First Experience of a Multicenter External Quality Assessment of Molecular 16S rRNA Gene Detection in Bone and Joint Infections

Detection of Prosthetic Joint Infection by Use of PCR-Electrospray Ionization Mass Spectrometry Applied to Synovial Fluid

Contact Info

Product

Resources

About