Broad resistance to acetohydroxyacid-synthase-inhibiting herbicides in feral radish (<i>Raphanus sativus</i>
 L.) populations from Argentina

Pandolfo, Claudio Ezequiel; Presotto, Alejandro; Moreno, F. C.; Dossou, Ida; Migasso, Juan Pablo; Sakima, Ernesto; Cantamutto, Miguel Ángel

doi:10.1002/ps.4006

Cited by 31 publications

(67 citation statements)

References 36 publications

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“…Agronomy Maringá, v. 39, n. 3, p. 299-306, July-Sept., 2017 2016). Different levels of resistance to herbicides may be related to the position of the mutation sites in relation to the herbicide-coupling site Pandolfo et al, 2016). …”

Section: Results and Discussion Als Enzyme Activity And In Vitro Assamentioning

confidence: 99%

“…Mutation of the ALS gene can compromise the herbicide coupling to the target site of the enzyme and affect the weed control with ALS inhibitor herbicides . The Trp-574-Leu substitution is considered the most relevant mutation because it confers resistance to all five chemical groups of the ALS inhibitor herbicides (Pandolfo et al, 2016). However, this mutation has not been reported in resistant biotypes of R. sativus L. in Brazil; this can be considered the first identified case.…”

Section: Als Gene Sequencingmentioning

confidence: 98%

“…The partial sequence presented a single nucleotide change of TGG to TTG, which led to a Trp-574-Leu substitution in resistant biotypes (Figure 3). This mutation was identified in different weeds resistant to ALS inhibitor herbicides, including a recent discovery in biotypes of R. sativus L. (Pandolfo et al, 2016). Mutation of the ALS gene can compromise the herbicide coupling to the target site of the enzyme and affect the weed control with ALS inhibitor herbicides .…”

Section: Als Gene Sequencingmentioning

confidence: 99%

“…The mutation diagnosed here (Trp-574-Leu) is one of the most important because it confers resistance to all chemical groups of ALS inhibitor herbicides, thus reducing the control options for resistant biotypes (Pandolfo et al, 2016;Cechin et al, 2016).…”

Section: Als Gene Sequencingmentioning

confidence: 99%

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<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

et al. 2017

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ABSTRACT. Acetolactate synthase inhibitors are the main group of herbicides used in winter crops in Southern Brazil where their intensive use has selected for herbicide-resistant biotypes of radish. The resistance affects the efficacy of herbicides, and identifying the resistance mechanism involved is important for defining management strategies. The aim of this study was to elucidate the resistance mechanism of radish biotypes by quantifying the enzyme activity, ALS gene sequencing and evaluating the response of biotypes to iodosulfuron and imazethapyr herbicide application after treatment with a cytochrome P 450 monooxygenase inhibitor. The susceptible (B 1 ) and resistant (B 4 and B 13 ) biotypes were from wheat fields in the Northwest of Rio Grande do Sul State. The results demonstrated that the enzyme affinity for the substrate (K M ) was not affected in biotypes B 4 and B 13 but that the V max of the resistant biotypes was higher than that of biotype B 1 . The resistant biotypes showed no differential metabolic response to iodosulfuron and imazethapyr herbicides when inhibited by malathion and piperonyl butoxide. However, gene sequencing of ALS showed a mutation at position 574, with an amino acid substitution of tryptophan for leucine (Trp-574-Leu) in resistant biotypes.Keywords: Raphanus sativus, mechanism of resistence, ALS enzyme activity, gene mutation, metabolism.Mutação da Trp-574-Leu do gene ALS confere resistência de biótipos de nabo ao herbicida iodosulfurom e imazetapir RESUMO. Os inibidores da acetolactato sintase são o principal grupo de herbicidas usados em culturas de inverno do Sul do Brasil onde seu uso intenso selecionou biótipos resistentes de nabo. A resistência afeta a eficácia dos herbicidas, e a identificação do mecanismo de resistência envolvido é importante na definição de estratégias de manejo. O objetivo deste estudo foi elucidar o mecanismo de resistência em biótipos de nabo através da quantificação da atividade da enzima, sequenciamento do gene ALS e avaliar a resposta dos biótipos à aplicação do herbicida iodosulfurom e imazetapir após tratamento com inibidores do metabolismo da citocromo P 450 monooxigenase. Os biótipos suscetível (B 1 ) e resistentes (B 4 e B 13 ) eram de lavouras de trigo da região Noroeste do Estado do Rio Grande do Sul. Os resultados demonstraram que a afinidade da enzima pelo substrato (K M ) não foi afetada nos biótipos B 4 e B 13 , porém o V max dos biótipos resistentes foi superior quando comparado ao biótipo B 1 . Os biótipos resistentes não apresentaram resposta metabólica diferencial ao herbicida iodosulfurom e imazetapir quando inibidos pelo malathion e butóxido de piperolina. Entranto, o sequenciamento do gene ALS evidenciou mutação na posição 574 com uma substituição do aminoácido triptofano por leucina (Trp-574-Leu) nos biótipos resistentes. Palavras-chave:Raphanus sativus, mecanismo de resistência, atividade da enzima ALS, mutação gênica, metabolismo.

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Section: Results and Discussion Als Enzyme Activity And In Vitro Assamentioning

confidence: 99%

Section: Als Gene Sequencingmentioning

confidence: 98%

Section: Als Gene Sequencingmentioning

confidence: 99%

Section: Als Gene Sequencingmentioning

confidence: 99%

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<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

et al. 2017

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“…33 A notable phenomenon is that, in contrast to many other resistance-endowing amino acid mutations (except the Arg377 mutation) involved in ALS-resistant weeds, all weed species with Trp574 mutations have high resistance (>10-fold) to all tested ALS-inhibiting herbicides 15 . For example, Pandolfo et al 34 mentioned that, in feral radish (Raphanus sativus L.) populations, resistance to all the ALS-inhibiting herbicide families was conferred by the Trp574Leu mutation.…”

Section: Whole-plant Dose Responsementioning

confidence: 99%

A novel amino acid substitution Trp574Arg in acetolactate synthase (ALS) confers broad resistance to ALS‐inhibiting herbicides in crabgrass (Digitaria sanguinalis)

Mei

Gao

et al. 2017

Pest Management Science

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Target‐site resistance and cross‐resistance to ALS‐inhibiting herbicides in radish and wild radish biotypes from Brazil

et al. 2021

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Radish (Raphanus sativus L.) and wild radish (R. raphanistrum L.) are troublesome agricultural weeds in several areas worldwide, with populations resistant to acetolactate synthase (ALS) inhibitor herbicide. Information on the mechanisms of resistance is important for management and developing novel solutions to control resistant populations. The objective of this work was to determine the ALS‐resistance mechanisms in radish and wild radish biotypes resistant to ALS‐inhibiting herbicides. Resistance levels were determined by dose‐response curve using several rates of metsulfuron‐methyl herbicide in susceptible (JUC1) and resistant (JUC2, CRA3) radish, and susceptible (CAF1) and resistant (CAF2, CAF3) wild radish biotypes. The mechanism was evaluated by the in vitro activity of the ALS enzyme with imazapic herbicide and ALS gene sequencing. All resistant biotypes were insensitive to metsulfuron‐methyl and imazapic herbicides. The rate of metsulfuron‐methyl to reduce 50% growth of the plants (GR50) were 17‐ to 60‐fold (JUC2, CRA3) and 9‐ to 21‐fold (CAF2 and CAF3) higher than the susceptible biotypes of radish and wild radish, respectively. One‐point mutation in the ALS gene, Trp‐574‐Leu, was found in all resistant radish biotypes, whereas a second point mutation, Ala‐360‐Ser, was found in CAF2, CAF3, and JUC2 resistant biotypes. Changes in kinetic parameters of the ALS enzyme were found in all resistant biotypes evaluated, causing reduction of the herbicide affinity in the target‐site of action. Trp‐574‐Leu amino acid substitution resulted in resistance to sulfonylurea, imidazolinone, and triazolopyrimidine groups, with the survival greater than 75% of the plants, depending on the herbicide applied at labeled rate.

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Broad resistance to acetohydroxyacid-synthase-inhibiting herbicides in feral radish (Raphanus sativus L.) populations from Argentina

Cited by 31 publications

References 36 publications

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

<b>Mutation of Trp-574-Leu ALS gene confers resistance of radish biotypes to iodosulfuron and imazethapyr herbicides

A novel amino acid substitution Trp574Arg in acetolactate synthase (ALS) confers broad resistance to ALS‐inhibiting herbicides in crabgrass (Digitaria sanguinalis)

Target‐site resistance and cross‐resistance to ALS‐inhibiting herbicides in radish and wild radish biotypes from Brazil

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