Broadening genetic diversity inBrassica napuscanola: Development of canola-quality springB. napusfromB. napus×B. oleraceavar.alboglabrainterspecific crosses

Abstract

G. 2015. Broadening genetic diversity in Brassica napus canola: Development of canola-quality spring B. napus from B. napusB. oleracea var. alboglabra interspecific crosses. Can. J. Plant Sci. 95: 29Á41. The narrow genetic base in spring Brassica napus (AACC) canola is a limitation for continued improvement of this crop. This research focused on broadening of genetic diversity in spring canola by using B. oleracea (CC). Seeds of B. oleracea contain high levels of erucic acid and glucosinolates, which are undesired in canola. Therefore, inheritance of these traits and the prospect of developing spring canola with allelic diversity introgressed from B. oleracea were investigated in B. napus) B. oleracea interspecific progenies. Zero-erucic plants in F 2 generation occurred at a lower frequency than expected based on segregation involving only the C-genome erucic acid alleles. Selection in F 2 to F 3 focused on zero erucic acid, while focus in later generation was for low glucosinolate and B. napus plants. In the F 6 , 31% zero-erucic families had low glucosinolate content. Flow cytometry analysis of the F 8 families showed no significant difference from the B. napus parent. Genetic diversity analysis by using simple sequence repeat markers from the C-genome chromosomes showed that the F 8 families received up to 54% alleles from B. oleracea. The results demonstrate the feasibility of enriching genetic diversity in B. napus canola by using B. oleracea. . et Se´guin-Swartz, G. 2015. É largissement de la diversite´ge´ne´tique du canola Brassica napus: de´veloppement de B. napus de printemps de qualite´canola a`partir de croisements interspe´cifiques B. napusB. oleracea var. alboglabra. Can. J. Plant Sci. 95: 29Á41. L'e´troite base ge´ne´tique des varie´te´s de printemps de Brassica napus (AACC) de qualite´canola entrave l'ame´lioration de cette culture. La pre´sente recherche devait e´largir la diversite´ge´ne´tique du canola de printemps en recourant a`B. oleracea (CC). Les graines de B. oleracea renferment une grande quantite´d'acide e´rucique et de glucosinolates, deux compose´s qu'on ne souhaite pas retrouver dans le canola. C'est pourquoi les auteurs ont examineĺ 'he´re´dite´de ces caracte`res et la possibilite´de trouver un canola de printemps posse´dant une diversite´alle´lique introgresse´e de B. oleracea dans la proge´niture du croisement interspe´cifique B. napus)B. oleracea. Les plants sans acide e´rucique de la F 2 sont moins fre´quents que pre´vu quand la se´gre´gation ne repose que sur les alle`les de l'acide e´rucique du ge´nome C. La se´lection de la F 2 a`la F 3 se concentrait sur l'absence d'acide e´rucique, tandis que, dans les ge´ne´rations subse´quentes, elle insistait sur la faible teneur en glucosinolates et les plants de B. napus. Dans la F 6 , 31 % de familles sans acide e´rucique pre´sentaient une faible concentration de glucosinolates. L'analyse par cytome´trie de flux des familles de la F 8 ne re´ve`le aucune distinction majeure par rapport au parent B. napus. L'analyse de la diversite´ge´...

Patterns of Heterosis in Three Distinct Inbred Populations of Spring Brassica napus Canola

“…The use of winter canola in breeding of spring hybrid cultivar can be advantageous as no stringent selection for zero erucic acid in oil and low glucosinolate content in seed meal (Kebede et al, 2010;Rahman, 2011;Rahman and Kebede, 2012) is needed as well as no difficulty to be encountered for the development of euploid (2n = 38) B. napus inbred lines from this type of crosses. This is in contrast to the use of allied species in breeding of hybrid-parents which encounter much difficulty for the development of a canola quality euploid line (Bennett et al, 2012; for review see Rahman, 2013;Rahman et al, 2015). Breeding in the past has always focused on selection for favorable (e.g., dominant) alleles for the development of winter and spring B. napus canola line cultivars and this might have eroded their contrasting (recessive) alleles from the breeding populations, which can potentially exhibit non-additive effect and contribute to heterosis.…”

“…S1); therefore, these 33 IN, 25 SS and 21 WS lines were used for production of test hybrids. Flow cytometric analysis of nuclear DNA content showed no significant difference between the IN lines and the B. napus parent Hi-Q (Rahman et al, 2015). synthesis method as implemented in PROC MIXED statement of SAS with the option of METHOD = Type3.…”

Potential loss of clubroot resistance genes from donor parent Brassica rapa subsp. rapifera (ECD 04) during doubled haploid production

“…While sowing of clubroot‐resistant cultivars is an important management strategy for controlling the disease (Diederichsen et al ., ; Peng et al ., ; Rahman et al ., ), spring B. napus canola in Canada has a narrow genetic background (Rahman et al ., ). Most commercial cultivars probably derived their resistance from the European winter B. napus ‘Mendel’.…”

Behind the scenes of microspore-based double haploid development in Brassica napus: A review

“…PI is excited at 488 nm and emits at a maximum wavelength of 617 nm. The quantity of DNA in each nucleus is correlated with the degree of PI fluorescence of cell nuclei (for protocol, see) [45].…”