Behind the scenes of microspore-based double haploid development in Brassica napus: A review

Rahman, Mukhlesur; Jiménez, Monika Michalak de

doi:10.7243/2050-2389-5-1

Cited by 12 publications

(7 citation statements)

References 52 publications

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“…Haploids refer to plants carrying one pair of chromosome (n) which is then doubled to produce homozygous DH (2n) plants. Use of haploids enhances genetic gains where homozygous lines can be attained within 2-3 generations compared to conventional methods which can take up to 8 generations (Figure 21) (Rahman and de Jiménez, 2016;Begheyn et al, 2016;Bakhtiar et al, 2014;Gordillo and Geiger, 2010).…”

Section: Doubled Haploid Breedingmentioning

confidence: 99%

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Awata¹,

Tongoona²,

Danquah³

et al. 2019

Int. J. Adv. Agric. Res.

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Maize is the second most important cereal crop in the world after wheat followed by rice. Although, it is among the latest entries in the list of food crops in Africa, maize has attracted much more attention in terms of research and adaptability. Consequently, maize has become the number 1 crop with significant contribution to modern farming and food security in sub-Saharan Africa (SSA). Majority of the population in the region depend on maize as their main source of calories, income and livelihood. Additionally, maize is of global importance as a model organism for advancement of genetic studies. However, maize production in the region is conditioned by complex factors leading to very low average yield compared to other parts of the world. General understanding of tropical maize is one of the key approaches required for improvement of tropical maize in SSA.Here an attempt was made to review various aspects of maize and major advances including the origin, taxonomy, genetics, morphology, physiology, cultural practices, yield potentials, breeding, and production constraints. The information generated could provide useful insights into tropical maize and might contribute towards enhancement of the crop for food security in SSA.

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Section: Doubled Haploid Breedingmentioning

confidence: 99%

Untitled

Awata¹,

Tongoona²,

Danquah³

et al. 2019

Int. J. Adv. Agric. Res.

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“…Genetic factors are fixed in DHs and will be identical in further generations, provided they are propagated in isolation. Therefore, every DH line can potentially become a cultivar (Cegielska--Taras et al, 2015;Rahman et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…The interaction between these parameters is high. Various antimitotic agents such as colchicine, antimicrotubule herbicides like oryzalin, trifluralin and amiprophos-methyl, and dinitroanilines, among others, are used to promote chromosome doubling to obtain homozygous, fertile DH plants (Hansen and Andersen, 1996;Rahman et al, 2016). Colchicine is still the most widely applied antimitotic agent both in vitro and in vivo, especially for agricultural plant species (Dhooghe et al, 2011;Rahman et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…Various antimitotic agents such as colchicine, antimicrotubule herbicides like oryzalin, trifluralin and amiprophos-methyl, and dinitroanilines, among others, are used to promote chromosome doubling to obtain homozygous, fertile DH plants (Hansen and Andersen, 1996;Rahman et al, 2016). Colchicine is still the most widely applied antimitotic agent both in vitro and in vivo, especially for agricultural plant species (Dhooghe et al, 2011;Rahman et al, 2016). In Brassica spp., colchicine can be applied at several stages of the androgenesis process, from isolated microspores (Cegielska-Taras et al, 2002) and microspore-derived embryos (Mohammadi et al, 2012) to the regenerated plants (Fletcher et al, 1998).…”

Section: Introductionmentioning

confidence: 99%

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Acta Biologica Cracoviensia s. Botanica

Szała¹,

Sosnowska²,

Cegielska-Taras³

2019

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The spontaneous diploidization rates in oilseed rape (Brassica napus L.) via in vitro androgenesis are too low for practical applications. In contrast, artificial doubling of chromosomes of the microspore has proven to be more successful and allows homozygous plants to be obtained in a short time. Here, we present the efficiency of diploidization of B. napus haploids using three different chromosome doubling methods. Using the in vitro approach in microspores, the rate of chromosome doubling in 24 populations of androgenic plants ranged from 15.8% to 94.0%. An alternative in vivo method for the induction of chromosome doubling involves colchicine treatment of young haploid plants, and this yielded doubling rates ranging from 47.5% to 86.4% in 10 different plant populations. Another in vivo method of chromosome doubling is colchicine treatment of the excised young axillary shoots of haploid plants at the early flowering stage. The high efficiency of this method was confirmed in haploid plant populations from 11 genetically distinct donors in which the frequency of occurrence of diploids ranged from 53.3% to 100%. However, in this case, the time required for seed formation from doubled haploids increased by about 3-5 months. The availability of several methods of chromosome doubling at various stages of the androgenic process -from isolated microspores through to young plants and flowering plants -allows seeds to be obtained from nearly every selected individual haploid.

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“…Одним из них является метод получения гаплоидов из репродуктивных клеток в условиях in vitro с последующим удвоением числа хромосом путем колхицинирования. Метод гаплоидии позволяет ускорить создание гомозиготных линий по сравнению с традиционной селекцией в 5-6 раз [16]. Гаплоидная биотехнология позволяет не только получить гомозиготные линии из гибридных комбинаций, но и использовать генетическое разнообразие микроспор для создания селекционноценного материала сельскохозяйственных культур [17].…”

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