Broccoli-shaped biosensor hierarchy for electrochemical screening of noradrenaline in living cells

Abstract

Monitoring and determination of ultra-trace concentrations of monoamine neurotransmitter such as noradrenaline (NA) in living cells with simple, sensitive and selective assays are significantly interesting. We design NA-electrode sensing system based on C-, N-doped NiO broccoli-like hierarchy (CNNB). The spherical broccoli-head umbrella architectures associated with nano-tubular arrangements enabled to tailor NA biosensor design. The homogenous doping and anisotropic dispersion of CN nanospheres along the entire NB head nanotubes lead to creating of abundant electroactive sites in the interior tubular vessels and outer surfaces for ultrasensitive detection of NA in living cells such as PC12. The CNNB biosensor electrodes showed efficient electrocatalytic activity, enhanced kinetics for electrooxidation of NA, and fast electron-transfer between electrode-electrolyte interface surfaces, enabling synergistic enhancement in sensitivity, and selectivity at a low-detectable concentration of ∼ 6nM and reproducibility of broccoli-shaped NA-electrodes. The integrated CNNB biosensor electrodes showed evidence of monitoring and screening of NA released from PC12 cells under K ion-extracellular stimulation process. The unique features of CNNB in terms of NA-selectivity among multi-competitive components, long-term stability during the detection of NA may open their practical, in-vitro application for extracellular monoamine neurotransmitters detection in living cells.

Characterization and Antibacterial Activity of Biosynthesized Silver Nanoparticles Using the Ethanolic Extract of Pelargonium sidoides DC

“…Several routes of synthesizing silver nanoparticles (AgNPs) have been reported previously, classified as chemical, physical, photochemical, and biological methods [4]. Synthesis of silver nanomaterials by means of chemical processes can be further subcategorized into chemical reduction methods, electrochemical techniques, photochemical methods, and pyrolysis whereas physical methods can be subcategorized into physical vapor condensation, inert gas condensation, cocondensation, ultraviolet irradiation, thermal decomposition, laser ablation arc-discharge, sonodecomposition, radiolysis, and direct current magnetron sputtering [5,6]. Synthesis and fabrication of nanoparticles using either chemical or physical methods pose a significant threat to the environment as their principal contaminants are difficult to purify and often require high energy input [7,8].…”

HMT-Controlled Synthesis of Mesoporous NiO Hierarchical Nanostructures and Their Catalytic Role towards the Thermal Decomposition of Ammonium Perchlorate

“…Accordingly, these materials are widely applied in many important fields. Among them, nickel oxide (NiO) is an important semiconductor functional material [2], which is widely used in lithium ion batteries [3], electrocatalysis [4], thermal catalysis [5], gas sensor [6], electrochemistry [7,8], optical sensor [9] and biosensors [10][11][12][13][14] fields. Therefore, the preparation of various mesoporous NiO nanomaterials has become a research hotspot.…”

Latest Trends in Electrochemical Sensors for Neurotransmitters: A Review

“…Several other groups have investigated catecholamine release by monitoring exocytosis using different approaches [164,165,166,167,168,169]. The numerous studies carried out have gavin a great impulse to better understand the functions of the NTs but nevertheless they are not exhaustive.…”