Bromelain partitioning in two-phase aqueous systems containing PEO–PPO–PEO block copolymers

The effect of pH on bromelain partition from Ananas comosus by PEG4000/phosphate ATPS

“…It was observed that on the tie lines two (14% PEG/13% salt) and three (12.6% PEG/12.2% salt) at pH 11, the total protein from pineapple juice was concentrated in top phase by 25-62 folds. These values were more than partition coefficients obtained by Fileti et al (2009), Fischer et al (2007, Lopes et al (2009) and Rabelo et al (2004). After the batch ATPS process, the top phase sample of the best system was analyzed by SDS-PAGE for the determination of molecular weight and degree of purification of the enzyme (Fig.…”

“…On optimal condition of batch purification a 3-folds of enzyme recovery was obtained and, a purification factor of 6-folds was found for the continuous ATPS operating at 0.5 for top/total flow and 2 s for the time interval between the pulses (Fileti et al, 2009 and2010;Fisher et al, 2007). A study of bromelain partitioning in twophase aqueous systems containing PEO-PPO-PEO block copolymers was made an value 1.25 was the maximum purification factor found for the bromelain recovery from pineapple fruit via twophase aqueous extraction (Rabelo et al, 2004). Lopes et al (2009) purified a bromelain from pineapple juice in a continuous system of the membrane separation process.…”

Bromelain: Methods of Extraction, Purification and Therapeutic Applications

“…The aqueous two-phase systems consists of a polymer and a salt (or two polymers) and have been regarded as extensively used tools for extraction and purification of biomolecules (Rabelo et al 2004). It is low cost, rapid, possess reusable polymers (Johansson et al 2011), scalable (Asenjo and Andrews 2011) and can withstand high biomass load as compared to rest of the separation systems (Kaul 2001).…”

“…When thermodynamic equilibrium and ATPS were employed together for the purification of bromelain, purification fold came out to be among 25-62 (Ferreira et al 2011). Moreover, thermoseparation of bromelain resulted in active recovery of 79.5% with 1.25 purification fold (Rabelo et al 2004). …”

Liquid–liquid extraction of biomolecules: an overview and update of the main techniques

“…Enzyme activity recovery around 79.5%, purification factor around 1.25 and activity partition coefficient around 1.4 were obtained. 51 The addition of affinity ligands to the aqueous two-phase partitioning system can greatly enhance the partitioning of enzymes. With that in mind, the introduction of biospecific affinity ligands was discussed and reviewed by Xu and co-workers.…”