BrucELISA: an enzyme-antibody immunoassay for detection of Brucella abortus antibodies in milk: correlation with the Brucella ring test and with shedding of viable organisms

Boraker, David K.; Stinebring, Warren R.; Kunkel, Johannes

doi:10.1128/jcm.14.4.396-403.1981

Cited by 17 publications

(8 citation statements)

References 14 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Under our conditions, the ELISA with protein G showed excellent sensitivity (98.2%) with respect to culture, and its specificity was 100% when testing milk from Brucella-free cattle. These results support the findings of other authors on the value of the indirect ELISA as an alternative for the individual screening of brucellosis when milk is available (5,16,21). Although the ELISA with protein G showed a better sensitivity than PCR, a culture-positive animal was ELISA negative and PCR positive and all infected animals were detected only when the results of both tests were considered.…”

Section: Discussionsupporting

confidence: 91%

Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle

et al. 1995

View full text Add to dashboard Cite

A study was performed to evaluate the previously described PCR (C. Romero, C. Gamazo, M. Pardo, and I. López-Goñi, J. Clin. Microbiol. 33:615-617, 1995) for the diagnosis of brucellosis in dairy cattle. Milk samples from 56 Brucella milk culture-positive cattle and from 37 cattle from Brucella-free herds were examined for Brucella DNA by PCR and for specific antibodies by an indirect enzyme-linked immunosorbent assay (ELISA). The specificities of both tests were 100% when testing the milk samples from Brucella-free cattle. The milk samples from 49 infected cattle were positive by PCR (87.5% sensitivity), and 55 were positive by ELISA (98.2% sensitivity). A PCR-positive sample was negative by ELISA, and 7 ELISA-positive samples were PCR negative, yielding an observed proportion of agreement of 0.91 for the two tests. Although the results suggest that ELISA is a better screening test than PCR, the combined sensitivity of the two assays was 100%, and their simultaneous application could be more useful than one test alone for a rapid screening of brucellosis in dairy cattle.

show abstract

Section: Discussionsupporting

confidence: 91%

Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle

et al. 1995

View full text Add to dashboard Cite

show abstract

“…The possibility that extreme heat caused an overgrowth of contaminating bacteria that obscured the recovery of Brucella spp. cannot be excluded (Boraker, Stinebring, & Kunkel, 1981), even though the samples were placed in a cooler shortly after collection.…”

Section: Discussionmentioning

confidence: 99%

Identification of Brucella spp. in feral swine (Sus scrofa) at abattoirs in Texas, USA

Pedersen

Bauer

Olsen

et al. 2017

Zoonoses and Public Health

View full text Add to dashboard Cite

Various tissues, nasal swabs, urine and blood samples were collected from 376 feral swine at two federally inspected abattoirs in Texas during six separate sampling periods in 2015. Samples were tested for Brucella spp. by culture and serology. Brucella spp. were cultured from 13.0% of feral swine, and antibodies were detected in 9.8%. Only 32.7% of culture-positive feral swine were also antibody positive, and 43.2% of antibody-positive feral swine were culture positive. Approximately, the same number of males (14.0%) and females (12.1%) were culture positive, and slightly more males (10.5%) than females (8.7%) were antibody positive. Our results indicate that serology likely underestimates the prevalence of feral swine infected, and that those who come in contact with feral swine should be aware of the symptoms of infection with Brucella spp. to ensure prompt treatment.

show abstract

“…In comparison to the MRT-1, the ELISA which does not depend on clustering of fat globules, is considered sensitive and specific (THOEN et al, 1979;BORAKER et al, 1981). We tested whether the ability of the ELISA to detect Brucellu antibodies in tank milk depends on the type of anti-bovine conjugate used.…”

Section: Discussionmentioning

confidence: 99%

“…Mixing the three conjugates did not improve the sensitivity of the ELISA either. In addition, bovine milk IgM can react non-specifically or actually inhibit the detection of low concentrations of anti-Brucellu IgG (BORAKER et al, 1981;LAMB et '' MRT-1 = milk ring test using 1 ml milk; MRT-8 = milk ring test using 8 ml milk; SAT = serum agglutination test; CFT = complement fixation test; + = positive in the MRT-8; ND = not done. 1979).…”

Section: Discussionmentioning

confidence: 99%

“…T h e enzyme-linked immunosorbent assay (ELISA) used to detect antibodies against Brucella in milk from individual cows is sensitive and specific, and m a y also be suitable for the detection of antibodies against Brucella in tank milk (THOEN et al, 1979;HECK et al, 1980;BORAKER, 1981; OLIVER and COOPER, 1981;FORSCHNER et al, 1989). FORSCHNER and BUNCER (1986) improved the sensitivity of the ELISA by concentrating milk serum gammaglobulins with ammonium sulfate and were able t o detect antibodies against Brucella in tank milk comprising milk samples from 50 cows or more.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Enzyme Immunoassay Using Mouse Monoclonal Anti‐bovine Antibodies for the Detection of Brucella abortus Antibodies in Cow Milk

Bercovich

Taaijke

1990

Journal of Veterinary Medicine, Series B

View full text Add to dashboard Cite

Summary Individual milk samples and artificially constructed tank milk samples from cows with naturally occurring brucellosis were examined by the enzyme‐linked immunosorbent assay (ELISA) using sonicated B. abortus S‐99 antigen, and mouse monoclonal anti‐bovine IgM, IgA, and IgG1 conjugates. ELISA results were compared with the results of the milk ring test using either 1 ml milk (MRT‐1) or 8 ml milk (MRT‐8). The ELISA using mouse monoclonal anti‐bovine IgG1 conjugate was sensitive and specific. In testing individual milk samples and constructed tank milk samples containing milk with low titers in the MRT‐1 the ELISA was superior to the MRT‐1, and MRT‐8. In testing other milk samples, the ELISA was as sensitive or slightly less sensitive than the MRT‐8. From a total of 5,910 milk samples collected from cows free from brucellosis, only 24 (0.4 %) samples tested positive in the ELISA. All 500 tank milk samples collected from farms negative for brucellosis tested negative in the ELISA. We concluded that the ELISA is a good substitute for the MRT‐1 to detect antibodies against Brucella in milk from individual cows. When tank milk is tested for antibodies against Brucella, however, both the MRT‐8 and the ELISA should be used.

show abstract

BrucELISA: an enzyme-antibody immunoassay for detection of Brucella abortus antibodies in milk: correlation with the Brucella ring test and with shedding of viable organisms

Cited by 17 publications

References 14 publications

Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle

Evaluation of PCR and indirect enzyme-linked immunosorbent assay on milk samples for diagnosis of brucellosis in dairy cattle

Identification of Brucella spp. in feral swine (Sus scrofa) at abattoirs in Texas, USA

Enzyme Immunoassay Using Mouse Monoclonal Anti‐bovine Antibodies for the Detection of Brucella abortus Antibodies in Cow Milk

Contact Info

Product

Resources

About