Brucella abortus S19 and RB51 vaccine immunogenicity test: Evaluation of three mice (BALB/c, Swiss and CD-1®) and two challenge strains (544 and 2308)

Miranda, Karina Leite; Dorneles, Elaine Maria Seles; Pauletti, Rebeca Barbosa; Poester, Fernando Padilla; Lage, Andrey Pereira

doi:10.1016/j.vaccine.2014.11.056

Cited by 21 publications

(17 citation statements)

References 22 publications

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“…Criteria for inclusion in this study are detailed in Fig 1. A total of 117 publications that met the criteria were selected for inclusion in this study [14, 17, 18, 20–28, 32, 33, 38, 40–141]. Therefore, a total of 782 individual experiments were analyzed.…”

Section: Resultsmentioning

confidence: 99%

“…The mouse model is suitable for studying pathogenesis, host immune response, and vaccine protection [36,37]. However, experimental protocols for assessing vaccine efficacy using this animal model are not standardized, which generates results that are often not quite reproducible [38]. Balb/c is the most commonly used mouse strain, although other strains have also been used for vaccine experiments, namely CD1, C57BL/6, OF1, 129/Sv, Swiss, and, mixed/outbred [16].…”

Section: Introductionmentioning

confidence: 99%

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Meta-Analysis and Advancement of Brucellosis Vaccinology

et al. 2016

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Background/ObjectivesIn spite of all the research effort for developing new vaccines against brucellosis, it remains unclear whether these new vaccine technologies will in fact become widely used. The goal of this study was to perform a meta-analysis to identify parameters that influence vaccine efficacy as well as a descriptive analysis on how the field of Brucella vaccinology is advancing concerning type of vaccine, improvement of protection on animal models over time, and factors that may affect protection in the mouse model.MethodsA total of 117 publications that met the criteria were selected for inclusion in this study, with a total of 782 individual experiments analyzed.ResultsAttenuated (n = 221), inactivated (n = 66) and mutant (n = 102) vaccines provided median protection index above 2, whereas subunit (n = 287), DNA (n = 68), and vectored (n = 38) vaccines provided protection indexes lower than 2. When all categories of experimental vaccines are analyzed together, the trend line clearly demonstrates that there was no improvement of the protection indexes over the past 30 years, with a low negative and non significant linear coefficient. A meta-regression model was developed including all vaccine categories (attenuated, DNA, inactivated, mutant, subunit, and vectored) considering the protection index as a dependent variable and the other parameters (mouse strain, route of vaccination, number of vaccinations, use of adjuvant, challenge Brucella species) as independent variables. Some of these variables influenced the expected protection index of experimental vaccines against Brucella spp. in the mouse model.ConclusionIn spite of the large number of publication over the past 30 years, our results indicate that there is not clear trend to improve the protective potential of these experimental vaccines.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Meta-Analysis and Advancement of Brucellosis Vaccinology

et al. 2016

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“…Despite the cost of the vaccine being just one fraction of the total cost of a control program, its quality will affect directly and dramatically the outcome of the program. Assessing the quality of live Brucella vaccines is usually based on in vitro criteria, including physico-chemical and microbiological in vitro tests as to purity, dissociation, and determination of pH, humidity and count of viable bacteria [ 9 ]. Recently, genetic stability has also been proposed as an additional criterion in assessing of the quality of Brucella spp.…”

Section: Vaccines Vaccination and Their Use In Brucellosis Contromentioning

confidence: 99%

Recent advances in Brucella abortus vaccines

Dorneles

Sriranganathan

Lage

2015

Vet Res

149

116

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Brucella abortus vaccines play a central role in bovine brucellosis control/eradication programs and have been successfully used worldwide for decades. Strain 19 and RB51 are the approved B. abortus vaccines strains most commonly used to protect cattle against infection and abortion. However, due to some drawbacks shown by these vaccines much effort has been undertaken for the development of new vaccines, safer and more effective, that could also be used in other susceptible species of animals. In this paper, we present a review of the main aspects of the vaccines that have been used in the brucellosis control over the years and the current research advances in the development of new B. abortus vaccines.

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“…Immunogenicity test performed in mouse is another alternative to assess the quality of bovine brucellosis vaccines, however as RB51 does not have cutoff points, i.e. defined protection zones [1,42], specific parameters of protection must be established.…”

Section: Bovine Brucellosis Control and Eradication Programsmentioning

confidence: 99%

Brucella Abortus Vaccines: Use in Control Programs and Immune Response

Lage¹

2017

J Bacteriol Mycol

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Brucella spp. is a highly infectious pathogen that affects numerous livestock and wild animal species besides humans, and that although eradicated in some countries, remains one of the most economically important zoonosis worldwide. Strain 19 and RB51 are the two Brucella abortus vaccine strains more largely used in the control of brucellosis in cattle worldwide, being effective in the prevention of abortion and infection, besides offering long lasting protection. Here, we reviewed the main aspects of the B. abortus vaccines and their use in brucellosis control and eradication programs over the years and the current understanding in the immune response triggered by these two B. abortus vaccines, especially in cattle.

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Brucella abortus S19 and RB51 vaccine immunogenicity test: Evaluation of three mice (BALB/c, Swiss and CD-1®) and two challenge strains (544 and 2308)

Cited by 21 publications

References 22 publications

Meta-Analysis and Advancement of Brucellosis Vaccinology

Meta-Analysis and Advancement of Brucellosis Vaccinology

Recent advances in Brucella abortus vaccines

Brucella Abortus Vaccines: Use in Control Programs and Immune Response

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