Brush technique in ocular surface cytology

Comparison of 3 corneal cytology collection methods for evaluating equine ulcerative keratitis: Cytobrush, kimura platinum spatula, and handle edge of scalpel blade

“…Collecting samples for cytological evaluation and microbial testing is routine practice in investigating the underlying cause or extent of the lesion . Cytologic examination is a safe, practical, and relatively inexpensive diagnostic tool that provides useful information for efficient assessment and guidance of therapy . Based on the cellular population and identification of infectious organisms, an initial diagnosis can be made .…”

“…Based on the cellular population and identification of infectious organisms, an initial diagnosis can be made . Accurate and timely diagnoses are critical for instituting appropriate therapy to provide the best prognosis for the patient …”

“…As part of a complete evaluation, cytology should complement a fungal and aerobic microbial culture with susceptibility . Microbial culture is more sensitive than cytology for the confirmation and identification of infectious organisms but results are not available immediately such as with cytology .…”

Nuclear parameters and chromatin remodeling in epithelial cells and lymphocytes from the palpebral conjunctiva of dogs with keratoconjunctivitis sicca

“…Brush cytology was performed as previously described. [32][33][34] Cells were harvested from the inferior palpebral conjunctival epithelium because it is easy to access, with less risk of accidentally damaging the cornea. The eyes were anesthetized with one drop of 0.5% proxymetacaine (Anestalconâ, Alcon, Sao Paulo, Brazil), and the inferior palpebral conjunctiva was carefully pulled down and scraped 4 times using a sterile cervical cytobrush (Kolplast Ltda, Itupeva, Brazil).…”

“…The median values of STT1 were 25.00 mm/min (range, [16][17][18][19][20][21][22][23][24][25][26][27][28][29][30][31][32][33][34][35] for the control group and 2.90 mm/min (range, 0-9) for the KCS group (P < .001). In relation to the TBUT, the median values were 10.00 seconds (range, 1-25) for the control group and 0.00 second (range, 0-8) for the KCS group (P < .001).…”