Bryophyllum pinnatum inhibits detrusor contractility in porcine bladder strips—A pharmacological study towards a new treatment option of overactive bladder

Schuler, Valerie; Suter, Katja; Fürer, Karin; Eberli, Daniel; Horst, Maya; Betschart, Cornelia; Brenneisen, Rudolf; Hamburger, Matthias; Mennet, Mónica; Martin, Sebastian; Simões-Wüst, Ana Paula; Mandach, U von

doi:10.1016/j.phymed.2012.05.003

Cited by 27 publications

(24 citation statements)

References 25 publications

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“…[24] Additional support for an antimuscarinic effect of the plant extracts was provided by its relaxation of isolated guinea pig ilei and porcine bladder strips precontracted with Ach or carbachol. [25,26] The apparent anti-histaminergic effect of the plant extracts noted in this study is in accordance with its ability to reduce the force of contraction of isolated pig ilei caused by histamine. [25,27] The H1 receptor antagonistic activity of the extracts has been supported by antiulcer activity using an ethanolic extract of the leaves of the plant showed significant (P < 0.001) activity at 400 mg/kg against histamineinduced ulcerations.…”

Section: Discussionsupporting

confidence: 80%

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Mamillapalli¹

2017

AJP

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Aim: This investigation has been conducted to evaluate the antiasthmatic activity and phytochemical characterization using gas chromatography-mass spectrum (GC-MS) analysis of the leaf extracts of Talinum portulacifolium. Materials and Methods: Hydroalcoholic and acetone extracts of the plant were prepared. Preliminary phytochemical screening has been conducted. Antiasthmatic activity was determined by two experimental models. In vivo methods, histamine and acetylcholine (Ach)-induced bronchospasm in guinea pigs were studied. Pre convulsion time (PCT) was calculated. In vitro, experimental methods such as histamine and Ach-induced contractions in ileum were also studied. Percentage inhibition of contractions was calculated. Phytochemical characterization was studied using GC-MS analysis. Results and Discussion: In histamine and Ach-induced bronchospasm studies acetone extracts of the plant have significantly increased PCT 10.69 and 10.52 (**P < 0.01), one-way analysis of variance (ANOVA) Tukey's test compared with control. Histamine and Ach-induced ileum contraction studies also showed that the acetone extracts exhibited response 2.6 with 47% and 2.2 with 40% inhibition (*P < 0.05). The results were expressed by one-way ANOVA, Dunnett's test. The results of GC-MS analysis depicted following phytoconstituents with major peak area, namely, 79.29% methoxy-bis (cyclopentadiene), 2. 83% -5,10-dihexyl-5,10-dihydroindolo[3,2-b]indole-2,7-dicarbaldehyde, and 1.84% -1,2-bis[3,4-dimethoxy benzyl]-1,2-bis (methoxymethyl) ethane. Conclusion: The results of this study clearly indicate that the hydroalcoholic and acetone extracts of T. portulacifolium can be used as promising antiasthmatic agents. The activity may be due to the presence of phytochemicals reported through GC-MS.

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Section: Discussionsupporting

confidence: 80%

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Mamillapalli¹

2017

AJP

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“…Two phenolic acids, several flavonol and O-methylated flavone glycosides, a bufadienolide, and lysophosphatidylcholine derivatives were identified for the first time in this plant which is currently the object of clinical investigations in different therapeutic indications [10,11]. Two flavonol glycosides were new natural products.…”

Section: Resultsmentioning

confidence: 95%

“…pinnatum leaf press juice also inhibits porcine detrusor contractility in vitro [10]. To explore the potential of B. pinnatum as a treatment for patients suffering from overactive bladder syndrome, a pilot study in humans was performed.…”

Section: Introductionmentioning

confidence: 99%

Two New Flavonol Glycosides and a Metabolite Profile of Bryophyllum pinnatum, a Phytotherapeutic Used in Obstetrics and Gynaecology

et al. 2013

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Bryophyllum pinnatum is a succulent perennial plant native to Madagascar which is used in anthroposophical medicine to treat psychiatric disorders and as a tocolytic agent to prevent premature labour. We performed a metabolite profiling study in order to obtain a comprehensive picture of the constituents in B. pinnatum leaves and to identify chromatographic markers for quality control and safety assessment of medicinal preparations. Preliminary HPLC-PDA-ESIMS analyses revealed that flavonoid glycosides were the main UV-absorbing constituents in the MeOH extract of B. pinnatum. Two phenolic glucosides, syringic acid -D-glucopyranosyl ester (1) and 4'-O--D-glucopyranosyl-cis-p-coumaric acid (2), as well as nine flavonoids (3-11) including kaempferol, quercetin, myricetin, acacetin, and diosmetin glycosides were unambiguously identified by 1H and 2D NMR analysis after isolation from a MeOH extract. The flavonol glycosides quercetin 3-O--L-arabinopyranosyl-(1 → 2)--L-rhamnopyranoside 7-O--D-glucopyranoside (3) and myricetin 3-O--L-arabinopyranosyl-(1 → 2)--L-rhamnopyranoside (4) were new natural products. With the aid of HPLC-PDA-APCIMS and authentic references isolated from the related species B. daigremontianum, the presence of four bufadienolides, bersaldegenin-1-acetate (12), bryophyllin A (13), bersaldegenin-3-acetate (14), and bersaldegenin-1,3,5-orthoacetate (15) AbstractBryophyllum pinnatum is a succulent perennial plant native to Madagascar which is used in anthroposophical medicine to treat psychiatric disorders, and as a tocolytic agent to prevent premature labour. We performed a metabolite profiling study in order to obtain a comprehensive picture of the constituents in B. pinnatum leaves, and to identify chromatographic markers for quality control and safety

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“…In addition, the press juice (10 %) had a significant relaxant effect (18.7 %) on carbachol-induced contractions. The leaf press juice showed good activity, although oxybutynin had a more potent inhibitory and relaxing effect on the detrusor muscle [75]. The effect of leaf press juice and different fractions of B. pinnatum on electrically induced porcine detrusor contractility was investigated in further experiments.…”

Section: Overactive Bladder (Oab) Syndromementioning

confidence: 99%

Bryophyllum pinnatum and Related Species Used in Anthroposophic Medicine: Constituents, Pharmacological Activities, and Clinical Efficacy

et al. 2016

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ReviewsThis document was downloaded for personal use only. Unauthorized distribution is strictly prohibited.Bryophyllum (see below) [4]. While these reviews describe various compounds and bioactivities, none of them addresses the pharmacological and clinical data that support the therapeutic use of Bryophyllum preparations in European countries. The present review focuses on B. pinnatum, but the few data available on Bryophyllum daigremontianum, Bryophyllum delagoense, and the hybrid Bryophyllum daigremontianum x tubiflorum have also been included. The German homeopathic pharmacopeia (HAB) 2014 [5] lists the two species B. pinnatum and B. daigremontianum as officinal in its monography "Bryophyllum Rh", and both have been used in AM. B. delagoense was introduced in the 1980s as an anthroposophic medicinal product in Germany, primarily for sedative purposes (Personal communication, Dr. med. Siegward-M. Elsas, see Acknowledgments). Botany

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Bryophyllum pinnatum inhibits detrusor contractility in porcine bladder strips—A pharmacological study towards a new treatment option of overactive bladder

Cited by 27 publications

References 25 publications

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Two New Flavonol Glycosides and a Metabolite Profile of Bryophyllum pinnatum, a Phytotherapeutic Used in Obstetrics and Gynaecology

Bryophyllum pinnatum and Related Species Used in Anthroposophic Medicine: Constituents, Pharmacological Activities, and Clinical Efficacy

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