Bryostatin-1 Specifically Inhibits In Vitro IgE Synthesis

Rabah, Dania; Grant, Steven; Ma, Check; Conrad, Daniel H.

doi:10.4049/jimmunol.167.9.4910

Cited by 11 publications

(7 citation statements)

References 48 publications

(43 reference statements)

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“…1b). 21,22 Switching to IgE is not affected by increasing the B‐cell dose. Both germline transcription (data not shown) and expression of the mature IgE transcript on the cell surface (Fig.…”

Section: Discussionmentioning

confidence: 99%

Effect of cell density on in vitro mouse immunoglobulin E production

Rabah

Conrad

2002

Immunology

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Summary In vitro immunoglobulin E (IgE) production was found to be sensitive to increasing cell concentration in culture wells. While class switching to IgE is intact as suggested by surface IgE staining, ELISPOT analysis provided evidence that the differentiation of IgE committed B cells to the plasma cell stage was arrested at high cell doses. In fact, splitting the cells at higher concentrations after culture initiation increased IgE production. Cells plated at higher doses were found to be more prone to apoptosis as assessed by Annexin staining. Interestingly, inhibiting apoptosis by the use of the caspase inhibitor DEVD significantly increased IgE levels implicating apoptosis in the preferential deletion of IgE expressing cells. These data not only highlight the caveat against using a single B‐cell dose for IgE production in vitro but also suggest for the first time a possible IgE regulatory mechanism mediated by cell density.

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Section: Discussionmentioning

confidence: 99%

Effect of cell density on in vitro mouse immunoglobulin E production

Rabah

Conrad

2002

Immunology

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“…PKC activity, with the active site usually located at the Cterminus to phosphorylate serine and threonine residues, is mediated by a family of at least twelve PKC isoforms [20][21][22] (Fig. 1).…”

Section: Protein Kinase C Isoforms and Molecular Targetsmentioning

confidence: 99%

Protein Kinase C Isozymes: Memory Therapeutic Potential

Sun

Alkon²

2005

CDTCNSND

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PKC plays an important role in many types of learning and memory. Evidence has been provided that PKC activation and translocation are induced in associative learning tasks. PKC inhibition, on the other hand, impairs learning and memory, consistent with the observations that transgenic animal models with a particular PKC isoform deficit exhibit impaired capacity in cognition. The dramatic impact of PKC pharmacology on learning and memory is further emphasized by a regulatory role of PKC isozymes in amyloid production and accumulation. Recent study reveals that PKC activation greatly reduces neurotoxic amyloid production and accumulation. PKC activators, therefore, may have important therapeutic values in the treatment of dementia, especially when fine-tuning of selective isoform activity can be effectively achieved pharmacologically, with further development of isozymes-specific agents. The success of antidementia therapy with agents that act on PKC signaling cascades depends on whether such agents at their effective doses would significantly disrupt or interfere with other vital functions that rely on a narrow range of PKC activities.

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“…IgM, IgG and IgE levels were measured as described previously 33. Micro‐titer plates were coated overnight at 4 °C with anti‐human IgE Ab, anti‐human IgG and anti‐human IgM, respectively.…”

Section: Methodsmentioning

confidence: 99%