BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1

Wang, Peng; Sun, Juan; Sun, Chengming; Zhao, Haoran; Zhang, YuBao; Chen, Jing

doi:10.1080/15384047.2023.2225884

Cited by 7 publications

(3 citation statements)

References 44 publications

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“…Heat shock protein 90 (HSP90) promotes cell glycolysis, proliferation and inhibits apoptosis by regulating PKM2 abundance in HCC ( 51 ). As an important molecule which controls the rate of glucose transport, high levels of GLUT1 are associated with the proliferation and invasiveness of liver cancer ( 52 ). The present study indicated that the expression levels of the glycolytic key proteins correlated positively with the expression of CD13 in Huh7, HepG2, and PLC/PRF/5 cells.…”

Section: Discussionmentioning

confidence: 99%

Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer

Shi,

Guo,

Jing

et al. 2023

Transl Cancer Res

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Background Liver cancer ranks third in fatalities among all cancer-related deaths. As a traditional chemotherapy drug, the application of cis-Diamminedichloroplatinum (II) (cisplatin, CDDP) for the treatment of liver cancer is greatly limited by its side effects and high drug resistance. Therefore, we are in urgent need of a more effective and less toxic CDDP therapeutic regimen. Our research aimed to clarify the possible mechanism of ubenimex in enhancing the effect of CDDP on liver cancer. Methods The underlying mechanism was determined using Cell Counting Kit-8 (CCK-8) assay, flow cytometry, immunofluorescence, enzyme-linked immunosorbent assay (ELISA), transwell assay, wound healing assay and western blot assay. Results The data indicated that ubenimex suppressed the expression levels of glycolysis-related proteins by decreasing the expression levels of cluster of differentiation 13 (CD13), while overexpression of CD13 could restore the activity of glycolysis. The glycolysis inhibitor 2-deoxy-D-glucose enhanced the antiproliferative effect of ubenimex and CDDP. In addition, the inhibition of the activity levels of the Hedgehog (Hh) pathway members was accompanied by a decrease in CD13 expression, which was reversed following CD13 overexpression. Moreover, ubenimex inhibited the production of lactic acid and adenosine triphosphate (ATP), as well as the expression of key proteins involved in glycolysis, which was similar to the effects caused by the Hh inhibitor cyclopamine. However, the effects of ubenimex were mediated by targeting CD13, while cyclopamine exhibited no effects on CD13, suggesting that Hh signaling occurred in the downstream of CD13. The inhibition of glycolysis by cyclopamine was reduced following CD13 overexpression, which further indicated that ubenimex targeted the CD13/Hh pathway to inhibit glycolysis. Finally, wound healing and transwell assays and cell proliferation and apoptosis analysis demonstrated that ubenimex inhibited glycolysis by alleviating the CD13/Hh pathway, which in turn enhanced the effects of CDDP on inhibiting the progression of liver cancer. Conclusions Ubenimex inhibits glycolysis by targeting the CD13/Hh pathway, thus playing an anti-tumor role together with CDDP. This study demonstrated the adjuvant effect of ubenimex from the perspective of Hh signal-dependent glycolysis regulation.

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Section: Discussionmentioning

confidence: 99%

Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer

Shi,

Guo,

Jing

et al. 2023

Transl Cancer Res

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“…Basic transcription factor 3 (BTF3) can transactivate FOXM1 to regulate the expression of GLUT1. Furthermore, BTF3 knockdown inhibits the proliferation of HCC cells and the glycolysis in HCC cells through the FOXM1/GLUT1 axis ( 19 ). Similarly, the antisense lncRNA SLC2A1-AS1 can regulate HCC cell proliferation and metastasis by competitively binding to the transcription factor STAT3, which inhibits the transcriptional activation of FOXM1 ( 20 ).…”

Section: Glucose Metabolic Reprogramming Affects Hccmentioning

confidence: 99%

Research progress in the metabolic reprogramming of hepatocellular carcinoma (Review)

Gao,

Wang,

et al. 2024

Mol Med Rep

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Hepatocellular carcinoma (Hcc) is the most common primary liver malignancy and its morbidity is increasing worldwide due to increasing prevalence. Metabolic reprogramming has been recognized as a hallmark of cancer and serves a role in cancer progression. Glucose, lipids and amino acids are three major components whose altered metabolism can directly affect the energy production of cells, including liver cancer cells. nutrients and energy are indispensable for the growth and proliferation of cancer cells, thus altering the metabolism of hepatoma cells can inhibit the progression of Hcc. The present review summarizes recent studies on tumour regulatory molecules, including numerous noncoding rnas, oncogenes and tumour suppressors, which regulate the metabolic activities of glucose, lipids and amino acids by targeting key enzymes, signalling pathways or interactions between the two. These regulatory molecules can regulate the rapid proliferation of cancer cells, tumour progression and treatment resistance. it is thought that these tumour regulatory factors may serve as therapeutic targets or valuable biomarkers for Hcc, with the potential to mitigate Hcc drug resistance. Furthermore, the advantages and disadvantages of metabolic inhibitors as a treatment approach for Hcc, as well as possible solutions are discussed, providing insights for developing more effective treatment strategies for Hcc. Contents 1. introduction 2. Glucose metabolic reprogramming affects Hcc 3. lipid metabolic reprogramming affects Hcc 4. regulation of amino acid metabolism affects Hcc 5. discussion

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“…Transcription factors play important roles in governing plant responses to the environment and regulating growth [1]. The functions of several transcription factors are universal during plant growth and response [2][3][4]. The DNA binding with one finger (Dof) family comprises plant-specific transcription factors, characterized by a single zinc finger structure, which were first found in maize [5,6].…”

Section: Introductionmentioning

confidence: 99%

Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis

Li,

Wang,

Jiang

et al. 2023

IJMS

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PEAR proteins are a type of plant-specific DNA binding with one finger (Dof) transcription factors that play a key role in the regulation of plant growth, especially during phloem cell growth and seed germination in Arabidopsis. However, the identification, characteristics and function of PEAR proteins, particularly in woody plants, need to be further studied. In the present study, 43 candidate PEAR proteins harboring the conserved Zf-Dof domain were obtained in Populus yunnanensis. Based on phylogenetic and structural analysis, 10 representative PEAR candidates were selected, belonging to different phylogenetic groups. The functions of PEAR proteins in the stress response, signal transduction, and growth regulation of stem cambium and roots undergoing vigorous cell division in Arabidopsis were revealed based on their expression patterns as characterized by qRT-PCR analysis, in accordance with the results of cis-element analysis. In vitro experiments showed that the interaction of transcription factor (E2F) and cyclin indirectly reflects the growth regulation function of PEAR through light signaling and cell-cycle regulation. Therefore, our results provide new insight into the identity of PEAR proteins and their function in stress resistance and vigorous cell division regulation of tissues in P. yunnanensis, which may serve as a basis for further investigation of the functions and characteristics of PEAR proteins in other plants.

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BTF3 promotes proliferation and glycolysis in hepatocellular carcinoma by regulating GLUT1

Cited by 7 publications

References 44 publications

Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer

Ubenimex suppresses glycolysis mediated by CD13/Hedgehog signaling to enhance the effect of cisplatin in liver cancer

Research progress in the metabolic reprogramming of hepatocellular carcinoma (Review)

Function and Characteristic Analysis of Candidate PEAR Proteins in Populus yunnanensis

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