Bubble-column design for growth of fragile insect cells

Tramper, J.; Smit, Dirk; Straatman, J.; Vlak, Just M.

doi:10.1007/bf00372858

Cited by 89 publications

(38 citation statements)

References 6 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Kilburn and Webb (1968) demonstrated the protective effects of Pluronic F68 in moderating cell damage. Tramper et al (1987) suggested the concept of a killing volume that was associated with bubble frequency and independent of rise height, and therefore, cell damage was primarily as a result of bubble disengagement. Handa et al (1987) and later Handa-Corrigan et al (1989) formalized these concepts into an experimental framework and showed that cell damage occurs due to bubble bursting, and that the amount of damage was related to the bubble size and frequency and that cell damage could be reduced by the addition of Pluronic F68.…”

Section: -63 Bubble-induced Cell Damage In Sparged Suspension Cultmentioning

confidence: 98%

“…After nearly two decades of research, it is now generally accepted that mechanical damage of freely suspended cells is due to bubble hydrodynamics, in particular the bubble-bursting phenomena at the headspace gas-liquid interface (Handa et al, 1987;Tramper et al, 1987;Handa-Corrigan et al, 1989;Oh et al, 1989Oh et al, , 1992Chalmers and Bavarian, 1991;Kioukia et al, 1992;Meier et al, 1999). Four bubble-liquid-cell regions where cell damage can occur can be identified: (1) bubble formation at the sparger, (2) bubble coalescence and breakup in the impeller discharge, (3) bubble rise through the bioreactor, and (4) bubble bursting at the air-medium interface.…”

Section: -63 Bubble-induced Cell Damage In Sparged Suspension Cultmentioning

confidence: 99%

See 1 more Smart Citation

Mixing in the Fermentation and Cell Culture Industries

Amanullah¹,

Buckland²,

Nienow

2003

Handbook of Industrial Mixing

View full text Add to dashboard Cite

Section: -63 Bubble-induced Cell Damage In Sparged Suspension Cultmentioning

confidence: 98%

Section: -63 Bubble-induced Cell Damage In Sparged Suspension Cultmentioning

confidence: 99%

Mixing in the Fermentation and Cell Culture Industries

Amanullah¹,

Buckland²,

Nienow

2003

Handbook of Industrial Mixing

View full text Add to dashboard Cite

“…Thus, the extent of cell death is proportional to the aeration rate Q and the specific volume in the wake of rising bubbles V K , and is inversely proportional to the diameter of the reactor d R , the height of fluid h L and the bubble diameter d B [35,36]. In this context, bubble rupture is the event with the most effect on cell viability.…”

Section: Aeration-related Shear Stressmentioning

confidence: 99%

The components of shear stress affecting insect cells used with the baculovirus expression vector system

Weidner

Druzinec

Mühlmann

et al. 2017

Zeitschrift Für Naturforschung C

View full text Add to dashboard Cite

Insect-based expression platforms such as the baculovirus expression vector system (BEVS) are widely used for the laboratory-and industrial-scale production of recombinant proteins. Thereby, major drawbacks to gain high-quality proteins are the lytic infection cycle and the shear sensitivity of infected insect cells due to turbulence and aeration. Smaller bubbles were formerly assumed to be more harmful than larger ones, but we found that cell damage is also dependent on the concentration of protective agents such as Pluronic ® . At the appropriate concentration, Pluronic forms a layer around air bubbles and hinders the attachment of cells, thus limiting the damage. In this context, we used microaeration to vary bubble sizes and confirmed that size is not the most important factor, but the total gas surface area in the reactor is. If the surface area exceeds a certain threshold, the concentration of Pluronic is no longer sufficient for cell protection. To investigate the significance of shear forces, a second study was carried out in which infected insect cells were cultivated in a hollow fiber module to protect them from shear forces. Both model studies revealed important aspects of the design and scale-up of BEVS processes for the production of recombinant proteins.

show abstract

“…The effects of bubble size and frequency and reactor geometry on cell damage can be predicted successfully (Tramper et al, 1988;Wang et al, 1994); however, the effects of medium composition have not been successfully accounted for. Certain nonionic surfactants, such as Pluronic F-68, are known to decrease damage, but attempts to correlate cell damage with the static surface tension or bulk viscosity have not been successful (Michaels and Papoutsakis, 1991).…”

Section: Introductionmentioning

confidence: 98%