Buccal Mucosa Exfoliative Cell Prussian Blue Stain Co-Relates with Iron Overload in β-Thalassemia Major Patients

Gajaria, Pooja Kamlesh; Maheshwari, Ujwala; Borkar, D B; Dhar, Reeta; Pancholi, Varsha

doi:10.1007/s12288-017-0801-4

Cited by 6 publications

(1 citation statement)

References 6 publications

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“…The PC3 cells were investigated under a light microscope (Olympus IX51; Olympus, Tokyo, Japan) at 10X and 40× magnifications. The AGS cell line (human epithelial stomach) was applied as a negative control cell (25,26).…”

Section: Oriented Conjugation Of Purified Polyclonal Anti-psca Antibody To Spionsmentioning

confidence: 99%

In-vitro prostate cancer biomarker detection by directed conjugation of anti-PSCA antibody to super paramagnetic iron oxide nanoparticless

Farahani

Mohsenzadegan

Taeb

et al. 2019

Med. J. Islam. Republ. Iran

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There are a few studies that have assessed the oriented conjugation methods (Covalent binding via carbohydrate moieties on the FC region of antibody). The conjugation of anti-hCG antibody to paramagnetic nanoparticles is one of them which was tested successfully. →What this article adds:In this study the oriented conjugation method was done through carbohydrate moieties of the FC domains for anti-PSCA antibody and dextran-superparamagnetic iron oxide nanoparticles for the first time in the world. In comparison with non-oriented conjugation, this method efficiently enhances the targeting of prostate tumor antigens in vitro.

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Section: Oriented Conjugation Of Purified Polyclonal Anti-psca Antibody To Spionsmentioning

confidence: 99%

In-vitro prostate cancer biomarker detection by directed conjugation of anti-PSCA antibody to super paramagnetic iron oxide nanoparticless

Farahani

Mohsenzadegan

Taeb

et al. 2019

Med. J. Islam. Republ. Iran

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Agedependent Changes in Human Buccal Epitelium

Semencova¹,

Полушина

Mandra

et al. 2020

Actual Problems in Dentistry

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Background. Most age-related changes are associated with the progression of functional instability in organs and tissues. This requires promising definitions of biological age and the pace of the human body development based on laboratory and instrumental assessment of the structure and functions of tissues. The article describes the potential of buccal cells investigations. The purpose was to compare the cytological characteristics of buccal epithelial cells in patients of various age groups (children, young people, the elderly and senile). Methodology. The study of the cytological features of buccal epithelial cells involved patients (men and women) in accordance with the WHO age classification, which were divided into 4 groups. The first group included pediatric patients (under 18 years old, 231 people), the second group included young patients (18―44 years old, 121 people), the 3rd group included elderly patients (60―74 years old, 16 people), and the fourth group included senile patients (75 ―90 years, 5 people). Results. The authors presented buccal epithelium application in non-invasive diagnosis of early human aging; identified common cytological features of buccal epithelium for different ages; revealed the accumulation of cytogenetic abnormalities (epithelial cells with micronuclei, protrusions of the nucleus) and degenerative-dystrophic changes (perinuclear vacuole, condensed chromatin, karyorexis, karyolysis) with age. These findings reflect the predominance of apoptosis over reparation in the process of aging. Conclusions. On this basis, it can be assumed that the buccal cytogram reflects age-dependent processes and can serve as an adequate tool for studying the mechanisms of aging. Among various methods exfoliative cytology is a unique, noninvasive technique involving simple and pain-free collection of intact cells from the oral cavity for microscopic examination.

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Influence of Age on Periodontal Human Health

Semencova

Bazarnyy

Mandra

et al. 2020

Actual Problems in Dentistry

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Subject. With increasing age of patients, in many cases, the severity of periodontal diseases also increases, and dystrophic ones join the inflammatory processes. Local predisposing factors in the oral cavity are aggravated by concomitant somatic diseases and a decrease in compensatory processes against the background of physiological aging. Literature data indicate that the manifestations of the physiological process of aging and pathological processes (inflammatory and dystrophic) can be clearly observed on the example of buccal epithelial cells. The aim is to identify the relationship between the age of patients, their periodontal status and the cytological characteristics of buccal epithelial cells. Methodology. All study participants were divided into two groups: the first included patients with a conditionally healthy periodontium (72 people), the second - with a chronic inflammatory process in the periodontium (57 people). In accordance with the WHO classification, a gradation of age was carried out: young (18―44 years old), mature (45―59 years old), elderly (60―74 years old), senile (75―90 years old). All patients underwent a comprehensive dental examination, sampling and cytological examination of the buccal epithelium, calculation of the integral indices of the buccal cytogram. Results. In healthy patients, a weak positive correlation was established between the cytogenetic index, the index of the accumulation of cytogenetic disorders and age, a weak negative correlation was found between the proliferative index and age. In patients with an inflammatory process in the periodontal tissues, a multidirectional change in the values of the buccal cytogram indices was observed, which may indicate an imbalance in the processes of regeneration, apoptosis, and cellular damage in inflammatory periodontal diseases in older age groups. Conclusions. In patients with healthy periodontal disease, a regular accumulation of cytogenetic disorders occurs with increasing age, while proliferative activity, on the contrary, decreases. Patients with inflammatory phenomena in the periodontal tissues are characterized by imbalance, impaired coordination of regeneration and apoptosis processes, combined with the most pronounced reactivity in the middle age period.

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Buccal Mucosa Exfoliative Cell Prussian Blue Stain Co-Relates with Iron Overload in β-Thalassemia Major Patients

Cited by 6 publications

References 6 publications

In-vitro prostate cancer biomarker detection by directed conjugation of anti-PSCA antibody to super paramagnetic iron oxide nanoparticless

In-vitro prostate cancer biomarker detection by directed conjugation of anti-PSCA antibody to super paramagnetic iron oxide nanoparticless

Agedependent Changes in Human Buccal Epitelium

Influence of Age on Periodontal Human Health

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