Bud-Poplar-Extract-Embedded Chitosan Films as Multifunctional Wound Healing Dressing

Abstract

Wounds represent a major global health challenge. Acute and chronic wounds are sensitive to bacterial infection. The wound environment facilitates the development of microbial biofilms, delays healing, and promotes chronic inflammation processes. The aim of the present work is the development of chitosan films embedded with bud poplar extract (BPE) to be used as wound dressing for avoiding biofilm formation and healing delay. Chitosan is a polymer with antimicrobial and hydrating properties used in wound dressing, while BPE has antibacterial, antioxidative, and anti-inflammatory properties. Chitosan-BPE films showed good antimicrobial and antibiofilm properties against Gram-positive bacteria and the yeast Candida albicans. BPE extract induced an immunomodulatory effect on human macrophages, increasing CD36 expression and TGFβ production during M1/M2 polarization, as observed by means of cytofluorimetric analysis and ELISA assay. Significant antioxidant activity was revealed in a cell-free test and in a human neutrophil assay. Moreover, the chitosan-BPE films induced a good regenerative effect in human fibroblasts by in vitro cell migration assay. Our results suggest that chitosan-BPE films could be considered a valid plant-based antimicrobial material for advanced dressings focused on the acceleration of wound repair.

A novel black poplar propolis extract with promising health-promoting properties: focus on its chemical composition, antioxidant, anti-inflammatory, and anti-genotoxic activities

“…96 In addition, the BPP extract S1 was also able to modulate ROS production, in line with previous studies in stimulated mammalian PMN. 97,98 These data support information obtained from the evaluation of antioxidant and antigenotoxic activity, and attest to the high quality of this product in its potential health benefits. Given the link between inflammatory status and oxidative stress with noncommunicable diseases (NCDs), 99,100 these results unquestionably merit further investigation.…”

Chitosan-Based Antibacterial Films for Biomedical and Food Applications

“…Chitosan films embedded with bud poplar extract demonstrated good antimicrobial and antibiofilm properties against Gram-positive bacteria and the yeast Candida albicans . Bud poplar extract induced an immunomodulatory effect on human macrophages, and this film induced a good regenerative effect in human fibroblasts by in vitro cell migration assay [ 110 ].…”