Europeanisation alters the conduct, content and norms of EU member states' foreign policies. This has also been true for Czech policy towards Eastern Europe, where the country actively supported EU frameworks and promoted European norms. With the rise of populists, however, the adherence to EU norms and structures has decreased to the extent that makes de-Europeanisation possible. This article analyses Czech policy towards Eastern Partnership countries after 2013 when populists entered the government. Based on three elements of potential de-Europeanisation, the adherence to European framework, professional norms and role of EU expertise, and deviation from foundational norms, the article concludes that Czech foreign policy towards the region remains highly Europeanised. Czech populists have concentrated on domestic matters and showed little interest in and understanding of foreign policy. Any changes to foreign policy will occur as a by-product of domestic politics, not a result of deliberate change in course in the future.