“…The following alleles were used in this study: FRT42D miR-7 Δ1 (a gift from R. W. Carthew, Northwestern University, Evanston, IL, USA), a null allele generated by excision of the miR-7 sequence, last two exons of bl and two exons of hillarin (Li and Carthew 2005), UAS-miR-7.140 (a gift from S. M. Cohen, Institute of Molecular and Cellular Biology, Singapore) (Stark et al, 2003), ttk69-RNAi (Vienna Drosophila RNAi Center), UAS-Ttk69 (Bloomington Stock Center BL7361), FRT82B ttk 1e11 (French et al, 2003) and -253/-39 lacZ (VM32E-lacZ) (Cavaliere et al, 2008). Notch reporters were E(spl)mβ-CD2 (de Celis et al, 1998), m7-lacZ (Assa-Kunik et al, 2007 and GbeL-lacZ (Furriols and Bray, 2001 Flies were maintained and raised at 25°C.…”