Bulgarian golden root in vitro cultures for micropropagation and reintroduction

Abstract

Abstract:Rhodiola rosea is an endangered medicinal plant used for cancer, cardiovascular, and nervous system diseases therapy. Due to its limited distribution and sustainability alternative methods for production of its valuable substances are under investigation. Using in vitro techniques apical and rhizome buds, leaf nodes, stem and radix segments from wild plants and in vitro seedlings were plated on 24 modified Murashige and Skoog (1962) media. Decontamination of plant material was successful only in 21% of the schemes. The best shoot induction was obtained from seedling explants on media containing 2 mg/l zeatin or N 6 -benzylaminopurine, each. Their reduction stimulated shoot formation in the next passages (multiplication rate up to 5). Efficient rooting was induced on half-strength MS with 2 mg/l Indole-3-butyric acid and stimulated by adding 0.2 mg/l Indolyl-3-acetic acid. Regenerants rooted in perlite, peat, and soil (1:1:2), adapted in greenhouse, and transplanted in the mountains survived (70%) and developed like the wild plants. Salidroside content of these plants after one or two years was high (0.64 and 0.61% in rhizomes and 0.62 and 0.53% in roots, respectively). This is the first established efficient scheme for micropropagation of Bulgarian R. rosea allowing habitats restoration, germplasm conservation, and potential application of biotechnology for production of valuable substances.

Basic procedure for the in vitro propagation of Brazilian trees for reforestation purposes

“…Sodium hypochlorite (NaOCl) has been widely used to successfully control bacteria and fungi on plant tissue surfaces (Emmanuel et al, 2004), for example in seeds of Rhodiola rosea L. (Tasheva & Kosturkova, 2012), Syngonanthus elegantulus Ruhland (Pêgo et al, 2013) and Byrsonima intermedia A. Juss. (Silva et al, 2015).…”

Rhodiola rosea L.: from golden root to green cell factories

“…R. rosea is protected species with endangered status in Great Britain, Czech Republic, Bosnia and Herzegovina (Platikanov and Evstatieva 2008); critically endangered in Bulgaria (Sidjimova et al 2014) and vulnerable in Slovakia (Galambosi et al 2006), where its collection is strictly forbidden (Platikanov and Evstatieva 2008). It is also a subject of protection in many former Soviet Union There are several strategies developed for the preservation of R. rosea, including field cultivation and micropropagation with the aim of restoring R. rosea to its natural habitats (Tasheva and Kosturkova 2010).…”

“…In vitro cultures from R. rosea are extensively used for biotransformation procedures in order to enhance the secondary metabolite production or for micropropagation of plants for the restoration of exhausted habitats (Tasheva and Kosturkova 2010). There are many factors affecting the in vitro culture induction from R. rosea, including plant ecotype, type of explants, nutrient medium compositions (plant growth regulators, carbon source and inorganic salts), light, temperature, and presence of precursors and elicitors, (Tasheva and Kosturkova 2010;Grech-Baran et al 2015). The available literature reveals that obtaining in vitro cultures from this plant is not an easy task and faces many difficulties starting from the sensitivity of the explants to disinfection agents to obtaining viable calli lines and producing the desired compounds (Ghiorghită et al 2011).…”

“…The available literature reveals that obtaining in vitro cultures from this plant is not an easy task and faces many difficulties starting from the sensitivity of the explants to disinfection agents to obtaining viable calli lines and producing the desired compounds (Ghiorghită et al 2011). The problem with the disinfection could be solved by short and soft sterilization procedure (3 to 6 min treatment with chloramine-T, 5%) or by using in vitro explants obtained from sterile seedlings (Ghiorghită et al 2011;Tasheva and Kosturkova 2010). The explant type can influence not only the callus morphology, but also its ability to produce secondary metabolites.…”

A Soft Computing QSAR Adapted Model for Improvement of Golden Rootin VitroCulture Growth

“…Protocols for in vitro plant organogenesis, regeneration and propagation using plants growing in nature were established previously (8). In vitro cultures with different characteristics were obtained using a large number of nutrient media and explants, allowing flexibility in research.…”