Bulges control pri-miRNA processing in a position and strand-dependent manner

Li, Shaohua; Le, Thi Nhu-Y; Nguyễn, Tùng Lâm; Trinh, Tam Anh; Nguyen, Tuan Anh

doi:10.1080/15476286.2020.1868139

Cited by 31 publications

(22 citation statements)

References 35 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…More generally, a role for ncRNA in neurodegenerative diseases is emerging [ 58 ]. Many lncRNAs were found to be differentially expressed in brains of AD patients or mouse models and involved in biological processes including Aß metabolism, tau phosphorylation, neuroinflammation, synaptic plasticity and neuronal death [ 26 ]. Our results provide evidence for LINC-PINT specifically in modulating CAA pathology in AD.…”

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis identifies a novel LINC-PINT splice variant associated with vascular amyloid pathology in Alzheimer’s disease

Reddy

Allen

et al. 2021

acta neuropathol commun

View full text Add to dashboard Cite

Cerebral amyloid angiopathy (CAA) contributes to accelerated cognitive decline in Alzheimer’s disease (AD) dementia and is a common finding at autopsy. The APOEε4 allele and male sex have previously been reported to associate with increased CAA in AD. To inform biomarker and therapeutic target discovery, we aimed to identify additional genetic risk factors and biological pathways involved in this vascular component of AD etiology. We present a genome-wide association study of CAA pathology in AD cases and report sex- and APOE-stratified assessment of this phenotype. Genome-wide genotypes were collected from 853 neuropathology-confirmed AD cases scored for CAA across five brain regions, and imputed to the Haplotype Reference Consortium panel. Key variables and genome-wide genotypes were tested for association with CAA in all individuals and in sex and APOEε4 stratified subsets. Pathway enrichment was run for each of the genetic analyses. Implicated loci were further investigated for functional consequences using brain transcriptome data from 1,186 samples representing seven brain regions profiled as part of the AMP-AD consortium. We confirmed association of male sex, AD neuropathology and APOEε4 with increased CAA, and identified a novel locus, LINC-PINT, associated with lower CAA amongst APOEε4-negative individuals (rs10234094-C, beta = −3.70 [95% CI −0.49—−0.24]; p = 1.63E-08). Transcriptome profiling revealed higher LINC-PINT expression levels in AD cases, and association of rs10234094-C with altered LINC-PINT splicing. Pathway analysis indicates variation in genes involved in neuronal health and function are linked to CAA in AD patients. Further studies in additional and diverse cohorts are needed to assess broader translation of our findings.

show abstract

Section: Discussionmentioning

confidence: 99%

Genome-wide analysis identifies a novel LINC-PINT splice variant associated with vascular amyloid pathology in Alzheimer’s disease

Reddy

Allen

et al. 2021

acta neuropathol commun

View full text Add to dashboard Cite

show abstract

“…Most miRNAs are derived from protein-coding genes in the human genome, but some miRNAs are derived from ncRNA-coding genes ( 26 ). Specifically, the genes encoding lncRNA are processed to form primary miRNA (pri-miRNA), and then pri-miRNA is further processed in the nucleus by Drosha/DGCR8 into a double-hairpin precursor miRNA (pre-miRNA) ( 27 ). Finally, the pre-miRNA is transported to the cytoplasm and cut into mature miRNA by Dicer ( 28 ).…”

Section: Ectopic Expression Of Lncrnas Mediates Chemotherapeutic Resistancementioning

confidence: 99%

The Non-Coding RNAs Inducing Drug Resistance in Ovarian Cancer: A New Perspective for Understanding Drug Resistance

Gong

Cao

et al. 2021

Front. Oncol.

View full text Add to dashboard Cite

Ovarian cancer, a common malignant tumor, is one of the primary causes of cancer-related deaths in women. Systemic chemotherapy with platinum-based compounds or taxanes is the first-line treatment for ovarian cancer. However, resistance to these chemotherapeutic drugs worsens the prognosis. The underlying mechanism of chemotherapeutic resistance in ovarian cancer remains unclear. Non-coding RNAs, including long non-coding RNAs, microRNAs, and circular RNAs, have been implicated in the development of drug resistance. Abnormally expressed non-coding RNAs can promote ovarian cancer resistance by inducing apoptosis inhibition, protective autophagy, abnormal tumor cell proliferation, epithelial-mesenchymal transition, abnormal glycolysis, drug efflux, and cancer cell stemness. This review summarizes the role of non-coding RNAs in the development of chemotherapeutic resistance in ovarian cancer, including their mechanisms, targets, and potential signaling pathways. This will facilitate the development of novel chemotherapeutic agents that can target these non-coding RNAs and improve ovarian cancer treatment.

show abstract

“…The sequence motifs UG at the basal junction, UGUG at the apical junction, and CNNC at the 3 0 flanking sequence have been reported to facilitate Drosha processing (Auyeung et al, 2013;Fang and Bartel, 2015). Recent studies have further found that miRNA precursors tend to have bulge-depleted regions in the upper and lower part of the miRNA duplex (Roden et al, 2017) and that bulges in the lower and middle part of the miRNA duplex influence Drosha processing efficiency and/or precision (Li et al, 2020b(Li et al, , 2020a. Other studies have shown that the GHG motif, defined as an unmatched nucleotide other than guanosine that is flanked by two base-paired guanosines at position À7 to À5 relative to the Drosha cleavage site, can facilitate miRNA precursor processing efficiency and precision (Fang and Bartel, 2015).…”

Section: Introductionmentioning

confidence: 99%