Burkholdines 1097 and 1229, Potent Antifungal Peptides from <i>Burkholderia ambifaria</i> 2.2N

Tawfik, Kamilia A.; Jeffs, Peter W.; Bray, Brian L.; Dubay, George R.; Falkinham, Joseph O.; Mesbah, Mostafa K.; Youssef, Diaa T. A.; Khalifa, Sherief; Schmidt, Eric W.

doi:10.1021/ol9029269

Cited by 60 publications

(44 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Many Burkholderia species exhibit a high potential for secondary metabolite production (Kang et al 1998; Partida-Martinez and Hertweck 2005; Partida-Martinez et al 2007; Knappe et al 2008; Schmidt et al 2009; Rohm et al 2010; Seyedsayamdost et al 2010; Tawfik et al 2010; Mahenthiralingam et al 2011; Franke et al 2012). As B. terrae BS001 possesses an exceptionally large genome, one might predict the ample presence of novel biosynthesis gene clusters.…”

Section: Resultsmentioning

confidence: 99%

The Genome of the Fungal-Interactive Soil Bacterium Burkholderia terrae BS001—A Plethora of Outstanding Interactive Capabilities Unveiled

Haq

Graupner

Nazir

et al. 2014

Genome Biology and Evolution

View full text Add to dashboard Cite

Burkholderia terrae strain BS001, obtained as an inhabitant of the mycosphere of Laccaria proxima (a close relative of Lyophyllum sp. strain Karsten), actively interacts with Lyophyllum sp. strain Karsten. We here summarize the remarkable ecological behavior of B. terrae BS001 in the mycosphere and add key data to this. Moreover, we extensively analyze the approximately 11.5-Mb five-replicon genome of B. terrae BS001 and highlight its remarkable features. Seventy-nine regions of genomic plasticity (RGP), that is, 16.48% of the total genome size, were found. One 70.42-kb RGP, RGP76, revealed a typical conjugal element structure, including a full type 4 secretion system. Comparative analyses across 24 related Burkholderia genomes revealed that 95.66% of the total BS001 genome belongs to the variable part, whereas the remaining 4.34% constitutes the core genome. Genes for biofilm formation and several secretion systems, under which a type 3 secretion system (T3SS), were found, which is consistent with the hypothesis that T3SSs play a role in the interaction with Lyophyllum sp. strain Karsten. The high number of predicted metabolic pathways and membrane transporters suggested that strain BS001 can take up and utilize a range of sugars, amino acids and organic acids. In particular, a unique glycerol uptake system was found. The BS001 genome further contains genetic systems for the degradation of complex organic compounds. Moreover, gene clusters encoding nonribosomal peptide synthetases (NRPS) and hybrid polyketide synthases/NRPS were found, highlighting the potential role of secondary metabolites in the ecology of strain BS001. The patchwork of genetic features observed in the genome is consistent with the notion that 1) horizontal gene transfer is a main driver of B. terrae BS001 adaptation and 2) the organism is very flexible in its ecological behavior in soil.

show abstract

Section: Resultsmentioning

confidence: 99%

The Genome of the Fungal-Interactive Soil Bacterium Burkholderia terrae BS001—A Plethora of Outstanding Interactive Capabilities Unveiled

Haq

Graupner

Nazir

et al. 2014

Genome Biology and Evolution

View full text Add to dashboard Cite

show abstract

“…This polyphasic taxonomic study revealed that strain MSh1 T represents a novel Bcc species, for which the name B. paludis is proposed. Based on current literature, Burkholderia species are known to produce narrow-spectrum antimicrobial compounds such as iminopyrrolidines (produced by B. plantari ) and occidiofungin ( B. contaminans ) which only targets Gram negative Erwinia amylovora and fungi, respectively (Lu et al, 2009; Tawfik et al, 2010). In this study, strain MSh1 T exhibit a different spectrum of antimicrobial activity, which targets Gram positive bacteria only, particularly strains of S. aureus and E. faecalis .…”

Section: Introductionmentioning

confidence: 99%

Burkholderia paludis sp. nov., an Antibiotic-Siderophore Producing Novel Burkholderia cepacia Complex Species, Isolated from Malaysian Tropical Peat Swamp Soil

Ong

Lee

et al. 2016

Front. Microbiol.

View full text Add to dashboard Cite

A novel Gram negative rod-shaped bacterium, designated strain MSh1T, was isolated from Southeast Pahang tropical peat swamp forest soil in Malaysia and characterized using a polyphasic taxonomy approach. The predominant cellular fatty acids (>10.0%) were C16:0 (31.7%), C17:0 cyclo (26.6%), and C19:0 cyclo ω8c (16.1%). The polar lipids detected were phosphatidylglycerol, phosphatidylethanolamine, and diphosphatidylglycerol. The predominant ubiquinone was Q-8. This revealed that strain MSh1T belongs to the genus Burkholderia. The type strain MSh1T can be differentiated from other Burkholderia cepacia complex (Bcc) species by phylogenetic analysis of 16S rRNA gene sequence, multilocus sequence analysis (MLSA), average nucleotide identity (ANI) and biochemical tests. DNA–DNA relatedness values between strain MSh1T and closely related type strains were below the 70% threshold value. Based on this polyphasic study of MSh1T, it can be concluded that this strain represents a novel species within the Bcc, for which the name Burkholderia paludis sp. nov. is proposed. The type strain is MSh1T (= DSM 100703T = MCCC 1K01245T). The dichloromethane extract of MSh1T exhibited antimicrobial activity against four Gram positive bacteria (Enterococcus faecalis ATCC 29212, E. faecalis ATCC 700802, Staphylococcus aureus ATCC 29213, S. aureus ATCC 700699) and a Gram negative bacteria (Escherichia coli ATCC 25922). Further purification work has led to the isolation of Compound 1, pyochelin. Pyochelin demonstrated antimicrobial activity against four S. aureus strains and three E. faecalis strains with MIC-values of 3.13 μg/ml and 6.26 μg/ml, respectively. SEM analysis showed that the cellular morphology of E. faecalis ATCC 700802 was not affected by pyochelin; suggesting that it might target the intracellular components. Pyochelin, a siderophore with antimicrobial activity might be useful in treating bacterial infections caused by S. aureus and E. faecalis, however further work has to be done.

show abstract

“…The genus Stenotrophomonas, part of the Gammaproteobacteria, is a member of the same family (Xanthomonadales) as Lysobacter, which is well known for antibiotic production (Xie et al 2012). The Betaproteobacteria isolated (Delftia, Acidovorax, Variovorax, and Achromobacter) are all Burkholderiales, which have recently showcased their biosynthetic potential with the discovery of several new bioactive compounds from Burkholderia, including the burkholdines and burkholdacs, as well as the recent discovery by our laboratory of a PK-NRP hybrid metallophore, delftibactin, produced by D. acidovorans (Biggins et al 2011;Johnston et al 2013;Lin et al 2012;Tawfik et al 2010;Xie et al 2012). Chitinophaga is the only known Bacteroidetes antibiotic producer isolated; however, Bacteroidetes have a high percentage of PKS and NRPS genes relative to their genome size, second only to the Actinobacteria, which may indicate the other isolated Bacteroidetes have unrealized biosynthetic potential and are a potential source for antimicrobial discovery (Donadio et al 2007).…”

Section: Resultsmentioning

confidence: 99%

Bioinformatic evaluation of the secondary metabolism of antistaphylococcal environmental bacterial isolates

et al. 2013

View full text Add to dashboard Cite

The increasing occurrence of drug-resistant Staphylococcus aureus is exacerbated with a declining rate of antibiotic discovery, particularly those with new mechanisms of action. The decline in antibiotic discovery from traditional sources, such as soil actinobacteria, necessitates examination of lesser studied microbes. Here, we present a strategy to select for organisms that may have a propensity to result in new antistaphylococcal agents by using S. aureus as a bait organism, and selecting organisms that have a natural lytic activity towards it. We have isolated over 80 environmental isolates and typed these organisms using 16S rDNA sequence comparison and deployed bioinformatics to assess the secondary metabolic potential of the isolated antistaphylococcal bacteria using genomic sequences. Bioinformatic analysis highlights the enriched and unique suite of potential antibiotic polyketides and nonribosomal peptides and lantibiotic gene clusters from these organisms. Profiling organic microbial extracts further showed that many of the organisms from the 10 staphylolytic genera secrete agents with antistaphylococcal activity and may serve as new sources for future antistaphylococcal drug discovery.

show abstract

Burkholdines 1097 and 1229, Potent Antifungal Peptides from Burkholderia ambifaria 2.2N

Cited by 60 publications

References 10 publications

The Genome of the Fungal-Interactive Soil Bacterium Burkholderia terrae BS001—A Plethora of Outstanding Interactive Capabilities Unveiled

The Genome of the Fungal-Interactive Soil Bacterium Burkholderia terrae BS001—A Plethora of Outstanding Interactive Capabilities Unveiled

Burkholderia paludis sp. nov., an Antibiotic-Siderophore Producing Novel Burkholderia cepacia Complex Species, Isolated from Malaysian Tropical Peat Swamp Soil

Bioinformatic evaluation of the secondary metabolism of antistaphylococcal environmental bacterial isolates

Contact Info

Product

Resources

About