Burkitt's cells can be triggered by teleocidin to secrete interferon‐γ

Abstract

The secretion of interferon (IFN)-gamma by T lymphocytes is mediated by the synthesis of interleukin 2 (IL-2) and the availability of IL-2 receptors. Since some Burkitt's lymphoma lines express Tac antigen and can be triggered to secrete IL-2 following activation with the new tumor promoter teleocidin, we addressed the question of whether the induction of IL-2 by B lymphocytes is accompanied by the induction of IFN-gamma. IFN-gamma has not been detected in any of the 25 cell lines studied, and following stimulation with teleocidin, we triggered the synthesis of IFN-gamma in JLP(C), a pre-Burkitt's cell line. The mechanism of IFN-gamma secretion by B lymphocytes is not clear. Our findings demonstrate that the synthesis of IL-2 by B cells is not accompanied by IFN-gamma and suggest that the synthesis of IFN-gamma is not mediated by IL-2 or IL-1 or B-cell growth factor. Neutralization studies have shown that IFN-gamma secretion is not accompanied by the induction of IFN-alpha or IFN-beta. Our data imply that B cells can be triggered to secrete IFN-gamma under certain circumstances. Whether similar function occurs in vivo is not known.

DIM stimulates IFNγ gene expression in human breast cancer cells via the specific activation of JNK and p38 pathways

“…It is known that IFNg is predominantly produced by natural killer (NK) cells during the early stages of immune responses, and by T cells during the adaptive stages of immune responses (Kasahara et al, 1983a, b). Besides NK cells and T cells, additional cell populations have been reported to express very low levels of IFNg mRNA, including B-cell lines, fibroblasts, and macrophages (Benjamin et al, 1986;Pang et al, 1992;McCaffrey et al, 1993;Dimarzio et al, 1994;Rady et al, 1995). The physiological relevance of IFNg expression in these cell types is unclear, and little work has been done to characterize the control of IFNg transcription in nonimmune cells.…”

Biomedical Potential of Marine Natural Products

“…Recently, teleocidin was used to study the regulation of tumor necrosis factor alpha as influenced by expression of interleukin-1 (IL-l) and IL-2 receptors, and interactions between B-celllymphokines (Benjamin et al, 1990). Teleocidins and TPA were also shown to simulate directly IL-2 function in cytotoxic T cells (Gaveriaux and Loor, 1989), to potentiate the activity of decay-accelerating factor (Bryant et al, 1990), and to stimulate B-cell gamma-interferon production (Benjamin et al, 1986).…”

Biologic and clinical significance of cytokine production in B‐cell malignancies

“…IL-2, a key cytokine for the immune system originally described as T-cell growth factor, is also an important promoter of proliferation and differentiation of normal B cells (10, ll), which express both heavy and light chains of IL-2 receptor. IL-2 is produced by T lymphocytes, mainly of the Thl subset (12), even though some neoplastic B-cell lines may release IL-2 under particular conditions (13). Its activity is mainly exerted in the early phases of B-cell maturation, and is potentiated by y-interferon (IF).…”