Introduction: Burnout syndrome is one of the many forms of suffering at work that affects healthcare professionals. It is still little diagnosed in Benin. Objectives: the objective was to assess the prevalence of burnout among the nursing staff of a Zone Hospital (HZ) in Cotonou. Study Methods: This was a descriptive and analytical cross-sectional study, which took place from September to October 2019 and included all personnel assigned to care. This has been submitted to the Malasch Burnout Inventory (MBI). The data collected was analyzed using STATA software version 15. A logistic regression made it possible to identify those associated with burnout. The significance level was 5%. Result: A total of 173 caregivers were included, including 118 women, for a sex ratio of 0.47. The mean age was 39 ± 10.1 years. The study population consisted of 33.5% nursing assistants, 24.3% nurses, 24.2% physicians, 7.5% midwives, and 8.6% other health professionals. The prevalence of burnout was 30.6% of which 2.3% were severe cases. As a result of burnout, 33% of officers and 19.08% experienced depersonalization and 10.4% experienced a sense of low personal achievement. The associated factors identified were female sex (p < 0.0001), lack of time devoted to her family (p = 0.04), receiving negative remarks on this lack of availability towards her family (p = 0.04), and work in a poor professional climate (p = 0.03). Conclusion: the prevalence of burnout among caregivers is high. Improving the professional climate and family relations are avenues for prevention.