Burst of Corneal Dendritic Cells during Trastuzumab and Paclitaxel Treatment

Sterenczak, Katharina A; Stache, Nadine; Bohn, Sebastian; Allgeier, Stephan; Köhler, Bernd; Bartschat, Andreas; George, Christian; Guthoff, Rudolf; Stachs, Oliver; Stachs, Angrit

doi:10.3390/diagnostics11050838

Cited by 12 publications

(22 citation statements)

References 30 publications

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“…The described real-time procedure is being used in several ongoing examination series 29 . Approval of the Ethics Committee of the University of Rostock in accordance with applicable laws, rules, and regulations was obtained on March 3, 2020 (A 2018-0162 with the amendment dated March 19, 2020).…”

Section: Resultsmentioning

confidence: 99%

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Real-time large-area imaging of the corneal subbasal nerve plexus

Allgeier

Bartschat

Bohn

et al. 2022

Sci Rep

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The morphometric assessment of the corneal subbasal nerve plexus (SNP) by confocal microscopy holds great potential as a sensitive biomarker for various ocular and systemic conditions and diseases. Automated wide-field montages (or large-area mosaic images) of the SNP provide an opportunity to overcome the limited field of view of the available imaging systems without the need for manual, subjective image selection for morphometric characterization. However, current wide-field montaging solutions usually calculate the mosaic image after the examination session, without a reliable means for the clinician to predict or estimate the resulting mosaic image quality during the examination. This contribution describes a novel approach for a real-time creation and visualization of a mosaic image of the SNP that facilitates an informed evaluation of the quality of the acquired image data immediately at the time of recording. In cases of insufficient data quality, the examination can be aborted and repeated immediately, while the patient is still at the microscope. Online mosaicking also offers the chance to identify an overlap of the imaged tissue region with previous SNP mosaic images, which can be particularly advantageous for follow-up examinations.

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Section: Resultsmentioning

confidence: 99%

“…In addition to the evaluation of the mosaic image size and quality, online mosaicking also offers the chance to identify an overlap of the imaged tissue region with previous SNP mosaic images immediately at the time of recording; this has proven to be particularly advantageous for follow-up examinations 29 .…”

Section: Discussionmentioning

confidence: 99%

Real-time large-area imaging of the corneal subbasal nerve plexus

Allgeier

Bartschat

Bohn

et al. 2022

Sci Rep

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“…Due to highresolution images, the same structures were easy to reidentify between various times and could be compared with each other. In the past, this tool was already used for longitudinal research on a breast cancer patient during Trastuzumab and Paclitaxel treatment (31). In previous CLSM studies of SNP migration, the inferocentral whorl was used as a landmark (18)(19)(20).…”

Section: Discussionmentioning

confidence: 99%

Assessment of dynamic corneal nerve changes using static landmarks by in vivo large-area confocal microscopy—a longitudinal proof-of-concept study

Stache¹,

Sterenczak²,

Sperlich³

et al. 2022

Quant Imaging Med Surg

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Background:The purpose of the present proof-of-concept study was to use large-area in vivo confocal laser scanning microscopy (CLSM) mosaics to determine the migration rates of nerve branching points in the human corneal subbasal nerve plexus (SNP).Methods: Three healthy individuals were examined roughly weekly over a total period of six weeks by large-area in vivo confocal microscopy of the central cornea. An in-house developed prototype system for guided eye movement with an acquisition time of 40 s was used to image and generate large-area mosaics of the SNP. Kobayashi-structures and nerve entry points (EPs) were used as fixed structures to enable precise mosaic registration over time. The migration rate of 10 prominent nerve fiber branching points per participant was tracked and quantified over the longitudinal period.Results: Total investigation times of 10 minutes maximum per participant were used to generate mosaic images with an average size of 3.61 mm 2 (range: 3.18-4.42 mm 2 ). Overall mean branching point migration rates of (46.4±14.3), (48.8±15.5), and (50.9±13.9) µm/week were found for the three participants with no statistically significant difference. Longitudinal analyses of nerve branching point migration over time revealed significant time-dependent changes in migration rate only in participant 3 between the last two measurements [(63.7±12.3) and (43.0±12.5) µm/week, P<0.01]. Considering individual branching point dynamics, significant differences in nerve migration rate from the mean were only found in a few exceptions. Conclusions:The results of this proof-of-concept study have demonstrated the feasibility of using in vivo confocal microscopy to study the migration rates of corneal subbasal nerves within large areas of the central human cornea (>1 mm 2 ). The ability to monitor dynamic changes in the SNP opens a window to future studies of corneal nerve health and regenerative capacity in a number of systemic and ocular diseases.Since corneal nerves are considered part of the peripheral nervous system, this technique could also offer an objective diagnostic tool and biomarker for disease-or treatment-induced neuropathic changes.

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“…The subjects in the GVHD group adapted the Japanese diagnostic criteria for DED. The details of this diagnostic criteria have been reported previously [13].…”

Section: Cgvhd Patientsmentioning

confidence: 99%

“…As a result, it is suitable to localize various cells and for grasping their shape. In addition, it is possible to comprehend the progress of the pathological condition and any therapeutic effects because IVCM can repeatedly observe the same point in the cornea without invasion [13]. Furthermore, recent studies have investigated the utility of an artificial intelligence-based deep learning algorithm with IVCM.…”

Section: Introductionmentioning

confidence: 99%

Observation of Chronic Graft-Versus-Host Disease Mouse Model Cornea with In Vivo Confocal Microscopy

et al. 2021

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Graft-versus-host disease (GVHD) is a major complication after hematopoietic stem cell transplantation (HSCT), and ocular GVHD can cause severe dry eye disease that can lead to visual impairment. Epithelial damage, vascular invasion, corneal fibrosis, and corneal perforation may occur in severe cases. It is generally accepted that inflammatory cells such as dendritic cells and T cells contribute to this pathological condition. However, it is still unknown what pathological condition occurs on the ocular surface after HSCT, and when. We therefore observed the dynamics of inflammatory cells in the cornea of chronic GVHD (cGVHD) model mice from 1 to 4 weeks after bone marrow transplantation (BMT) by in vivo confocal microscopy (IVCM) and considered the relationship with the pathophysiology of ocular GVHD (tear volume, corneal epithelial damage). In the allogeneic group, neovascularization occurred in all eyes at 1 week after BMT, although almost all vessels disappeared at 2 weeks after BMT. In addition, we revealed that infiltration of globular cells, and tortuosity and branching of nerves in the cornea occurred in both cGVHD mice and human cGVHD patients. Thus, we consider that cGVHD mouse model study by IVCM reproduces the state of ocular GVHD and may contribute to elucidating the pathological mechanism for ocular GVHD.

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Burst of Corneal Dendritic Cells during Trastuzumab and Paclitaxel Treatment

Cited by 12 publications

References 30 publications

Real-time large-area imaging of the corneal subbasal nerve plexus

Real-time large-area imaging of the corneal subbasal nerve plexus

Assessment of dynamic corneal nerve changes using static landmarks by in vivo large-area confocal microscopy—a longitudinal proof-of-concept study

Observation of Chronic Graft-Versus-Host Disease Mouse Model Cornea with In Vivo Confocal Microscopy

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