Bushen-Qiangdu-Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43

Zhou, Yingyan; Huang, Run‐Yue; Lin, Jiehua; Xu, Yong-Yue; He, Xinrong; He, Ying

doi:10.3892/etm.2016.3292

Cited by 5 publications

(7 citation statements)

References 31 publications

(40 reference statements)

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“…together with the findings summarized in Table 1, 16,77,[97][98][99][100][101][102][103][104] confirm that ASFs are target cells of BMP/TGF-β signaling and indicate that a highly activated BMP/TGF-β signal transduction pathway exists in ASFs. Concurrently, based on the evidence that the Smad6 expression is lower in ASFs than normal, it reveals the lack of self-regulation and inhibition in the BMP/TGF-β signaling of ASFs.…”

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confidence: 61%

Fibroblast Insights into the Pathogenesis of Ankylosing Spondylitis

Liu,

Cai,

et al. 2023

JIR

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Purpose of the Review: Emerging evidence has shown that ankylosing spondylitis fibroblasts (ASFs) act as crucial participants in inflammation and abnormal ossification in ankylosing spondylitis (AS). This review examines the investigations into ASFs and their pathological behavior, which contributes to inflammatory microenvironments and abnormal bone formation. The review spans the period from 2000 to 2023, with a primary focus on the most recent decade. Additionally, the review provides an in-depth discussion on studies on ASF ossification at the cellular level. Recent Findings: ASFs organize immune functions by recruiting immune cells and influencing their differentiation and activation, thus mediate the inflammatory response in the early phase of disease. ASFs promote joint destruction at sites of cartilage and actively promote abnormal ossification by recruiting osteoblasts, differentiation into myofibroblasts or ossification directly. Many signaling pathways and cytokines such as Wnt signaling and BMP/TGF-β signaling are involved in ASF ossification. Summary: ASFs play a key role in AS inflammation and osteogenesis. Further studies are required to elucidate molecular mechanisms behind that and provide new targets and directions for AS diagnosis and treatment from a new perspective of fibroblasts.

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confidence: 61%

Fibroblast Insights into the Pathogenesis of Ankylosing Spondylitis

Liu,

Cai,

et al. 2023

JIR

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“…Furthermore, miR-204-5p inhibited the osteogenic differentiation of AS fibroblasts by downregulating the expression of Notch2, RUNX2 and osteocalcin. Heterotopic ossification is one of the most prominent features of AS ( 29 ), and osteogenic differentiation of fibroblasts plays a key role in the heterotopic ossification of AS ( 30 ). miRNAs play important roles in regulating cell-cell interactions between osteoclasts and fibroblasts ( 31 ).…”

Section: Discussionmentioning

confidence: 99%

MicroRNA‑204‑5p inhibits the osteogenic differentiation of ankylosing spondylitis fibroblasts by regulating the Notch2 signaling pathway

Zhao

Zhang

Liu³

2020

Mol Med Rep

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ankylosing spondylitis (aS) is a chronic inflammatory systemic disease and is difficult to detect in the early stages. The present study aimed to investigate the role of microRNA (miR)-204-5p in osteogenic differentiation of AS fibroblasts. Bone morphogenetic protein 2 (BMP-2) was used to induce osteogenic differentiation. Cells were divided into the following groups: AS group, AS + BMP-2 group, AS + BMP-2 + miR-negative control group, AS + BMP-2 + miR-204-5p mimics group and AS + BMP-2 + miR-204-5p mimics + pcDNA-Notch2 group. The expression levels of miR-204-5p, Notch2, runt-related transcription factor 2 (RUNX2) and osteocalcin were detected via reverse transcription-quantitative PCR analysis. The binding site between Notch2 and miR-204-5p was predicted using TargetScan software and verified via the dual-luciferase reporter assay. Alkaline phosphatase (ALP) activity was assessed via the ALP assay, while the mineralized nodules area was determined via the Alizarin Red S staining assay. The results demonstrated that Notch2 is a target gene of miR-204-5p. Furthermore, treatment with BMP-2 significantly decreased miR-204-5p expression, and significantly increased ALP activity, the mineralized nodules area and the expression levels of Notch2, RUNX2 and osteocalcin in ligament fibroblasts (all P<0.05). Conversely, transfection with miR-204-5p mimics significantly increased miR-204-5p expression, and significantly decreased ALP activity, the mineralized nodules area and the expression levels of Notch2, RUNX2 and osteocalcin in ligament fibroblasts (all P<0.05). Notably, transfection with pcDNA-Notch2 significantly reversed the inhibitory effects induced by miR-204-5p mimics on the osteogenic differentiation of ligament fibroblasts (all P<0.05). Furthermore, miR-204-5p inhibited the osteogenic differentiation of ligament fibroblasts in patients with AS by targeting Notch2. Thus, miR-204-5p may negatively regulate Notch2 expression and may be a potential therapeutic target for AS. Collectively, the results of the present study provide a theoretical basis for the effective treatment of patients with AS.

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“…On the basis of the holistic concept and syndrome differentiation, TCM can be directed at different pathogenic links, using a variety of methods, and through multiple pathways, to achieve the effect of treating diseases and regulating immunity, and can alleviate the side effects of western medicine [23] . Therefore, integrated TCM and western medicine in the treatment of AS can synergize with each other to exert their own advantages, which can both remove multiple pathogenic factors of AS and improve the systemic status to achieve the best therapeutic effect [24] . At present, a host of clinical trials have shown that CHF combined with western medicine has good clinical effectiveness and safety in the treatment of AS [25] …”

Section: Introductionmentioning

confidence: 99%

“…[23] Therefore, integrated TCM and western medicine in the treatment of AS can synergize with each other to exert their own advantages, which can both remove multiple pathogenic factors of AS and improve the systemic status to achieve the best therapeutic effect. [24] At present, a host of clinical trials have shown that CHF combined with western medicine has good clinical effectiveness and safety in the treatment of AS. [25] Systematic review is the process of applying a clear methodology to find, select, and critically appraise relevant studies, from which data are extracted and combined using appropriate statistical methods to reach a comprehensive conclusion, with a view to providing evidence to address a specific clinical question.…”

Section: Introductionmentioning

confidence: 99%

Effectiveness and safety of Chinese herbal formula combined with western medicine for ankylosing spondylitis

Miao¹,

Deng²,

2021

Medicine

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Background: Ankylosing spondylitis (AS) is a chronic progressive inflammatory disease of the spine, which mainly invades the sacroiliac joint, spine, and large joints near the trunk, leading to fibrous and skeletal ankylosis and deformity, and can cause damage to the eyes, lung, cardiovascular, kidney and other organs. Chinese herbal formulas (CHF) is an important interventions of Traditional Chinese Medicine (TCM), and CHFs combined with western medicine are widely used in clinical practice to treat AS. Methods: Eight databases will be systematically retrieved from their inceptions to March 2021. Only randomized controlled trials (RCTs) of CHFs combined with western medicine for AS treatment will meet the inclusion criteria. The primary outcomes we focus on include clinical effectiveness rate, TCM syndrome score, TCM symptom score, Bath ankylosing spondylitis disease activity index (BASDAI), Bath Ankylosing Spondylitis Functional Index (BASFI), Bath Ankylosing Spondylitis Metrology Index (BASMI), chest expansion, nocturnal spinal pain, adverse reactions, erythrocyte sedimentation rate (ESR), and C protein response (CRP). The research screening, data extraction, and risk of bias assessment will be performed independently by 2 researchers, and divergence will be solved by a third researcher. Revman 5.3 software will be used for meta-analysis. The confidence of evidence will be graded using grading of recommendations assessment, development, and evaluation (GRADE) algorithm and methodological quality will be assessed adopting risk of bias in systematic reviews (ROBIS). Results: This systematic review (SR) will provide evidence-based medical evidence for AS therapy by CHF combined with western medicine and we will submit the findings of this SR for peer-review publication. Conclusions: This SR will provide latest and updated summary proof for assessing the effectiveness and safety of CHF combined with western medicine for AS. Registration number: INPLASY 202150089.

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Bushen-Qiangdu-Zhilv decoction inhibits osteogenic differentiation of rat fibroblasts by regulating connexin 43

Cited by 5 publications

References 31 publications

Fibroblast Insights into the Pathogenesis of Ankylosing Spondylitis

Fibroblast Insights into the Pathogenesis of Ankylosing Spondylitis

MicroRNA‑204‑5p inhibits the osteogenic differentiation of ankylosing spondylitis fibroblasts by regulating the Notch2 signaling pathway

Effectiveness and safety of Chinese herbal formula combined with western medicine for ankylosing spondylitis

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