Butanol production from alkali-pretreated rice straw by co-culture of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum

Kiyoshi, Keiji; Furukawa, Masashi; Seyama, Tomoko; Kadokura, Toshimori; Nakazato, Atsumi; Nakayama, Shunichi

doi:10.1016/j.biortech.2015.03.061

Cited by 78 publications

(30 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…As demonstrated in an earlier work, C. saccharoperbutylacetonicum strain N1-4 is highly suitable for butanol production from cellulosic substrates through coculturing with C. thermocellum (4,5). However, as shown in that earlier work and as confirmed in the present work, growth and butanol production by strain N1-4 cease at 37°C, in contrast to several other butanol-producing clostridia that grow normally and produce butanol at higher temperatures (6, 7).…”

Section: Discussioncontrasting

confidence: 47%

“…Cell densities were determined by using a spectrophotometer (U-2910 spectrophotometer; Hitachi Instruments Inc., Tokyo, Japan) to measure the absorbance of the samples at 600 nm. Levels of fermentation products and residual glucose were measured by high-performance liquid chromatography (HPLC), as described previously (4,5). The concentrations of nucleic acids in the culture supernatant were measured at 260 nm with an HPLC system equipped with a UV-visible detector (LC10-ADVP pump and SPD-10AVvp detector; Shimadzu, Kyoto, Japan), using an RSpak DE-413 column (150 mm by 4.6 mm by 4 m; Showa Denko, Tokyo, Japan).…”

Section: Methodsmentioning

confidence: 99%

“…talline cellulose and delignified rice straw through coculture of the thermophilic and cellulolytic Clostridium thermocellum with the mesophilic, butanol-producing, Clostridium saccharoperbutylacetonicum strain N1-4 (4,5). This coculture system includes both cellulase and butanol production phases, with butanol production proceeding via simultaneous saccharification and fermentation.…”

mentioning

confidence: 99%

See 2 more Smart Citations

Adenine Addition Restores Cell Viability and Butanol Production in Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) Cultivated at 37°C

Kiyoshi

Kawashima

Nobuki

et al. 2017

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

We have developed butanol-producing consolidated bioprocessing from cellulosic substrates through coculture of cellulolytic clostridia and butanol-producing Clostridium saccharoperbutylacetonicum strain N1-4. However, the butanol fermentation by strain N1-4 (which has an optimal growth temperature of 30°C) is sensitive to the higher cultivation temperature of 37°C; the nature of this deleterious effect remains unclear. Comparison of the intracellular metabolites of strain N1-4 cultivated at 30°C and 37°C revealed decreased levels of multiple primary metabolites (notably including nucleic acids and cofactors) during growth at the higher temperature. Supplementation of the culture medium with 250 mg/liter adenine enhanced both cell growth (with the optical density at 600 nm increasing from 4.3 to 10.2) and butanol production (increasing from 3.9 g/liter to 9.6 g/liter) at 37°C, compared to those obtained without adenine supplementation, such that the supplemented 37°C culture exhibited growth and butanol production approaching those observed at 30°C in the absence of adenine supplementation. These improved properties were based on the maintenance of cell viability. We further showed that adenine supplementation enhanced cell viability during growth at 37°C by maintaining ATP levels and inhibiting spore formation. This work represents the first demonstration (to our knowledge) of the importance of adenine-related metabolism for clostridial butanol production, suggesting a new means of enhancing target pathways based on metabolite levels. IMPORTANCE Metabolomic analysis revealed decreased levels of multiple primary metabolites during growth at 37°C, compared to 30°C, in C. saccharoperbutylacetonicum strain N1-4. We found that adenine supplementation restored the cell growth and butanol production of strain N1-4 at 37°C. The effects of adenine supplementation reflected the maintenance of cell viability originating from the maintenance of ATP levels and the inhibition of spore formation. Thus, our metabolomic analysis identified the depleted metabolites that were required to maintain cell viability. Our strategy, which is expected to be applicable to a wide range of organisms, permits the identification of the limiting metabolic pathway, which can serve as a new target for molecular breeding. The other novel finding of this work is that adenine supplementation inhibits clostridial spore formation. The mechanism linking spore formation and metabolomic status in butanol-producing clostridia is expected to be the focus of further research.

show abstract

Section: Discussioncontrasting

confidence: 47%

Section: Methodsmentioning

confidence: 99%

mentioning

confidence: 99%

See 1 more Smart Citation

Adenine Addition Restores Cell Viability and Butanol Production in Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) Cultivated at 37°C

Kiyoshi

Kawashima

Nobuki

et al. 2017

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Results for the co-culture of the C. thermocellum and C. saccharoperbutylacetonicum strain N1-4 on alkali-pretreated rice straw hydrolysate showed that 5.5 g/L butanol was produced during 7 days of ABE fermentation (Kiyoshi et al 2015).…”

Section: Abe Fermentation Using Acid-pretreated Pkcmentioning

confidence: 99%

Production of Acetone, Butanol, and Ethanol (ABE) by Clostridium acetobutylicum YM1 from Pretreated Palm Kernel Cake in Batch Culture Fermentation

et al. 2017

View full text Add to dashboard Cite

The viability of most fermentation processes is very much dependent on the cheap fermentation medium used. Palm kernel cake (PKC) is an abundant biomass generated from the palm oil processing industry that can be used as the carbon source for the growth and production of acetone-butanolethanol fermentation (ABE) by Clostridia. In this study, ABE production from the fermentation of PKC using Clostridium acetobutylicum YM1 in a batch culture was conducted. The PKC was subjected to treatment with acids (sulphuric and hydrochloric acids), alkali (sodium hydroxide and alkaline peroxide), enzymatic hydrolysis, and hydrothermal treatment (in autoclave). The sulphuric acid-treated PKC (2% SAPKC) method produced the highest concentration of reducing sugars (30 g/L) compared with the other methods applied. The results showed that increasing the concentration of H2SO4 up to 3% decreased the amounts of generated reducing sugars to 20.4 g/L, which is about 32% less. The fermentation of 1%, 2%, and 3% SAPKC resulted in the production of ABE of 1.07, 5.72, and 3.48 g/L, respectively. This study showed that the pretreatment of PKC improved the content of fermentable sugars and subsequently enhanced the production of ABE by C. acetobutylicum YM1. This study also revealed that PKC can be regarded as a potentially low cost substrate for ABE fermentation.

show abstract

“…Lignocellulose, such as that in rice straw, is composed of cellulose, hemicellulose and lignin and is considered to be well suited for use as a cost-effective and abundant substrate for biobutanol production [2,3]. We previously demonstrated how a co-culture of the cellulolytic Clostridium thermocellum and the butanol-producing Clostridium saccharoperbutylacetonicum strain N1-4 could be used to produce butanol from delignified rice straw, and how enhancing exoglucanase activity could be used to increase butanol production [4]. It is considered that promoting exoglucanase activity by the butanol-producing strain N1-4, which exhibits very little exoglucanase activity but possesses a host-vector system [5], could be used to cost effectively produce butanol from delignified rice straw.…”

Section: Introductionmentioning

confidence: 99%

Identification of Xylanase Signal Peptide in Culture Supernatant of Clostridium saccharoperbutylacetonicum Strain N1-4 Cultured on Delignified Rice Straw

Kubota¹,

Kiyoshi²,

Nobuki³

2015

J Microb Biochem Technol

Self Cite

View full text Add to dashboard Cite

Butanol production from alkali-pretreated rice straw by co-culture of Clostridium thermocellum and Clostridium saccharoperbutylacetonicum

Cited by 78 publications

References 20 publications

Adenine Addition Restores Cell Viability and Butanol Production in Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) Cultivated at 37°C

Adenine Addition Restores Cell Viability and Butanol Production in Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) Cultivated at 37°C

Production of Acetone, Butanol, and Ethanol (ABE) by Clostridium acetobutylicum YM1 from Pretreated Palm Kernel Cake in Batch Culture Fermentation

Identification of Xylanase Signal Peptide in Culture Supernatant of Clostridium saccharoperbutylacetonicum Strain N1-4 Cultured on Delignified Rice Straw

Contact Info

Product

Resources

About