Butin Attenuates Arthritis in Complete Freund’s Adjuvant-Treated Arthritic Rats: Possibly Mediated by Its Antioxidant and Anti-Inflammatory Actions

Alzarea, Sami I.; Alasmari, Abdullah F.; Alanazi, Abdullah Salah; Alzarea, Abdulaziz Ibrahim; Alharbi, Metab; Alshammari, Abdulrahman; Kazmi, Imran; Al-Joufi, Fakhria A.; Sayyed, Nadeem; Afzal, Muhammad

doi:10.3389/fphar.2022.810052

Cited by 9 publications

(3 citation statements)

References 43 publications

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“…Prostaglandins usually relax arteriolar muscle cells and improve blood flow, thus considerably amplifying the exudates. CFA induction elevates PGE-2 levels and is responsible for increased paw edema volume in arthritic rats (Alzarea et al, 2022). SsBu significantly reduced the elevated PGE-2 level, suggesting a response of SsBu against CFAinduced inflammation and arthritis.…”

Section: Discussionmentioning

confidence: 87%

Antiarthritic potential of the butanol fraction of Sesuvium sesuvioides: An in vitro, in vivo, and in silico evaluation

et al. 2023

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Sesuvium sesuvioides (Fenzl) Verdc (Aizoaceae) has been traditionally used in the treatment of inflammation, arthritis, and gout. However, its antiarthritic potential has not been evaluated scientifically. The current study was designed to assess the antiarthritic properties of the n-butanol fraction of S. sesuvioides (SsBu) by phytochemical analysis, in vitro and in vivo pharmacological activities, and in silico studies. Phytochemical analysis showed total phenolic contents (90.7 ± 3.02 mg GAE/g) and total flavonoid contents (23.7 ± 0.69 mg RE/g), and further analysis by GC-MS identified possible bioactive phytocompounds belonging to phenols, flavonoids, steroids, and fatty acids. The in vitro antioxidant potential of SsBu was assessed by DPPH (175.5 ± 7.35 mg TE/g), ABTS (391.6 ± 17.1 mg TE/g), FRAP (418.2 ± 10.8 mg TE/g), CUPRAC (884.8 ± 7.97 mg TE/g), phosphomolybdenum (5.7 ± 0.33 mmol TE/g), and metal chelating activity (9.04 ± 0.58 mg EDTAE/g). Moreover, in the in vitro studies, inhibition (%) of egg albumin and bovine serum albumin denaturation assays showed that the anti-inflammatory effect of SsBu at the dose of 800 μg/ml was comparable to that of diclofenac sodium used as a standard drug. The in vivo antiarthritic activity was assessed to determine the curative impact of SsBu against formalin-induced (dose-dependent significant (p < 0.05) effect 72.2% inhibition at 750 mg/kg compared to standard; 69.1% inhibition) and complete Freund’s adjuvant-induced arthritis (40.8%; inhibition compared to standard, 42.3%). SsBu significantly controlled PGE-2 level compared to the control group (p < 0.001) and restored the hematological parameters in rheumatoid arthritis. Treatment with SsBu significantly reduced oxidative stress by reinstating superoxide dismutase, GSH, and malondialdehyde along with pro-inflammatory markers (IL-6 and TNF-α) in arthritic rats. Molecular docking revealed the antiarthritic role of major identified compounds. Kaempferol-3-rutinoside was found to be more potent for COX-1 (−9.2 kcal/mol) and COX-2 inhibition (−9.9 kcal/mol) than diclofenac sodium (COX-1, −8.0 and COX-2, −6.5 kcal/mol). Out of the 12 docked compounds, two for COX-1 and seven for COX-2 inhibition showed more potent binding than the standard drug. The results from the in vitro, in vivo, and in silico approaches finally concluded that the n-butanol fraction of S. sesuvioides had antioxidant and antiarthritic potential, which may be due to the presence of potential bioactive compounds.

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Section: Discussionmentioning

confidence: 87%

Antiarthritic potential of the butanol fraction of Sesuvium sesuvioides: An in vitro, in vivo, and in silico evaluation

et al. 2023

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“…The paw volume was obtained using a plethysmometer to determine the inhibition (%) of edema in each treated group. 17,18 Days 0, 7, 14, 21, and 28 were the different time intervals used to measure the paw volume in each group (Figure 1). The mean% inhibition rate of edema 19,20 was used to express the anti-inflammatory activity of the applied materials.…”

Section: In-vivo Anti-arthritic Sudymentioning

confidence: 99%

Preclinical Assessment of Aceclofenac Transdermal Patch by Freund’s Complete Adjuvant-induced Arthritis in Rats

Bajaj¹,

Yadav²,

Singh³

et al. 2022

IJPQA

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>The hydroxypropyl methylcellulose (HPMC) based transdermal patches of aceclofenac were prepared. On the basis of in-vitro potential, the formulation was additionally chosen and assessed for their in-vivo anti-arthritic activity and acute skin irritation tests using the primary skin irritation (draize) test and freund’s complete adjuvant (FCA) caused arthritis technique. Furthermore, stability studies were conducted in compliance with ICH standards. The studied compositions had no irritant potential, and no edema production was ever noticed. All of the formulations had a zero irritation score (primary skin irritation index), indicating their safety and acceptability for transdermal administration. The stability investigations confirmed the produced patch’s stability, and the in-vivo analysis showed a considerable decrease in the volume of irritated paws compared to the marketed formulation. The created transdermal patches may serve as a platform for the delivery of medication for the treatment of arthritis

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“…Millions of people worldwide suffer from Rheumatoid Arthritis (RA), a chronic autoimmune disease that manifests as an intricate interplay of inflammatory processes in the synovial membrane and joints 1 .…”

Section: Introductionmentioning

confidence: 99%

Evaluating the Efficacy of a Polyherbal Formulation in Ameliorating Arthritis Induced by Complete Freund’s Adjuvant

Chitra,

Damodharan,

Narasimha

et al. 2024

JNR

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Background: This study investigates the antiarthritic potential of a polyherbal formulation (PHF) comprising extracts from Tinospora cordifolia, Rosa damescena, and Acacia leucoploea in a rat model of rheumatoid arthritis (RA). RA is a chronic autoimmune disorder with debilitating consequences. PHF's impact on joint inflammation, bone degradation, and cartilage preservation were evaluated. Methods: Arthritis was induced using complete Freund’s adjuvant (CFA), and animals were treated with PHF (200 and 400 mg/kg), prednisolone, or control treatments for 28 days. Parameters including body weight, paw volume, arthritis severity score, hematological parameters, serum markers (creatinine, ALP, total proteins), cytokine levels (IL-1β, IL-6, TNF-α, IL-10), and radiographic changes were assessed. Results: CFA-treated rats exhibited significant body weight loss, paw edema, increased arthritis severity scores, altered hematological parameters, and elevated serum markers compared to normal controls. PHF treatment at both doses mitigated body weight loss, reduced paw edema, and improved arthritis severity scores. Hematological changes induced by CFA were also attenuated by PHF treatment. Serum creatinine, ALP, and total protein levels, elevated in CFA-treated rats, were significantly improved by PHF. Furthermore, PHF modulated cytokine levels, decreasing IL-6, IL-1β, and TNF-α while increasing IL-10. Radiographic analysis displayed reduced joint damage in PHF-treated rats compared to CFA controls. Conclusion: This comprehensive investigation highlights PHF's potential to mitigate the inflammatory processes associated with RA, as evidenced by improved clinical, hematological, and biochemical parameters. The study underscores the promise of traditional botanical compounds in managing RA and suggests PHFs as novel therapeutic options. Further mechanistic studies are warranted to elucidate the exact pathways involved.

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Butin Attenuates Arthritis in Complete Freund’s Adjuvant-Treated Arthritic Rats: Possibly Mediated by Its Antioxidant and Anti-Inflammatory Actions

Cited by 9 publications

References 43 publications

Antiarthritic potential of the butanol fraction of Sesuvium sesuvioides: An in vitro, in vivo, and in silico evaluation

Antiarthritic potential of the butanol fraction of Sesuvium sesuvioides: An in vitro, in vivo, and in silico evaluation

Preclinical Assessment of Aceclofenac Transdermal Patch by Freund’s Complete Adjuvant-induced Arthritis in Rats

Evaluating the Efficacy of a Polyherbal Formulation in Ameliorating Arthritis Induced by Complete Freund’s Adjuvant

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