Butylated Hydroxytoluene (BHT) Inhibits PIN1 Exocytosis From BFA Compartments in Arabidopsis Roots

Paponov, Ivan A.; Budnyk, Vadym; Paponov, Martina; Teale, William; Palme, Klaus

doi:10.3389/fpls.2020.00393

Cited by 5 publications

(3 citation statements)

References 42 publications

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“…The regulation of PIN circulation by redox signaling has been supported by an investigation showing the inhibition of PIN2 exocytosis by the reactive oxygen species, hydrogen peroxide [60]. Our recent investigation showed that GSH had a reverse response to that induced by hydrogen peroxide [61], which supported the regulation of PIN2 trafficking by the redox status of the cells. A complex feedback loop between redox signaling, auxin, and PIN expression and localization may therefore be affecting the RAM, as redox homeostasis affects PIN expression and localization, while PINs modulate the auxin gradient in the RAM [30] and change auxin concentrations, whereas the modulation of auxin signaling in turns affects PIN expression [62].…”

Section: Gsh Level Affects Pin-modulated Auxin Transportmentioning

confidence: 63%

Glutathione Enhances Auxin Sensitivity in Arabidopsis Roots

2020

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Root development is regulated by the tripeptide glutathione (GSH), a strong non-enzymatic antioxidant found in plants but with a poorly understood function in roots. Here, Arabidopsis mutants deficient in GSH biosynthesis (cad2, rax1, and rml1) and plants treated with the GSH biosynthesis inhibitor buthionine sulfoximine (BSO) showed root growth inhibition, significant alterations in the root apical meristem (RAM) structure (length and cell division), and defects in lateral root formation. Investigation of the molecular mechanisms of GSH action showed that GSH deficiency modulated total ubiquitination of proteins and inhibited the auxin-related, ubiquitination-dependent degradation of Aux/IAA proteins and the transcriptional activation of early auxin-responsive genes. However, the DR5 auxin transcriptional response differed in root apical meristem (RAM) and pericycle cells. The RAM DR5 signal was increased due to the up-regulation of the auxin biosynthesis TAA1 protein and down-regulation of PIN4 and PIN2, which can act as auxin sinks in the root tip. The transcription auxin response (the DR5 signal and expression of auxin responsive genes) in isolated roots, induced by a low (0.1 µM) auxin concentration, was blocked following GSH depletion of the roots by BSO treatment. A higher auxin concentration (0.5 µM) offset this GSH deficiency effect on DR5 expression, indicating that GSH deficiency does not completely block the transcriptional auxin response, but decreases its sensitivity. The ROS regulation of GSH, the active GSH role in cell proliferation, and GSH cross-talk with auxin assume a potential role for GSH in the modulation of root architecture under stress conditions.

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Section: Gsh Level Affects Pin-modulated Auxin Transportmentioning

confidence: 63%

Glutathione Enhances Auxin Sensitivity in Arabidopsis Roots

2020

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“…While, synthetic antioxidants, such as butylated hydroxytoluene (BHT), have been correlated with many drawbacks, including liver damage and carcinogenesis. Therefore, to replace this synthetic antioxidant and avoid its side effects, natural sources have been extensively studied 44 .…”

Section: Antioxidant Activity Of Methanolic Extracts a Bruguierimentioning

confidence: 99%

Phytochemical profile, Antioxidant, Enzyme inhibitory and acute toxicity activity of Astragalus bruguieri

Jabbar,

Abdulrahman,

Abdulsamad

et al. 2023

Baghdad Sci.J

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The medicinal plants (Astragalus species) have been used traditionally as anti-inflammatory, antioxidant, and Anti-diabetics. The current research investigates the phytochemistry and some biological activity of methanol extract of different parts of Astragalus bruguieri Bioss., a wild medicinal plant grows on Safeen mountain, Erbil, Iraq. The methanol extracts of A. bruguieri were analyzed for total phenolic, flavonoid, and saponin contents. In-vitro antioxidant activity was analyzed by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis (3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays. Furthermore, the plant extracts were examined for in-vitro enzyme inhibitory activity and in-vivo sub-acute toxicity. The results have shown the highest total phenolic (28.83, 20.62 mg GAEs/g extracts) contents, in the leave and root extracts, respectively. While the highest total Flavonoid (50.08, 44.01 mg REs/g) contents, were found in the extracts of aerial parts and leaves, respectively. The total saponin was higher (25.33, 23.18 mg GAEs/g extracts) in the roots and aerial parts, respectively. In-vitro antioxidant measurement by (DPPH) assay showed leaves as superior part in this activity (42.19mg TEs/g extract), while antioxidant evaluation by (ABTS) assay indicated roots as the most active part (86.90mg TEs/g extract). The α-glucosidase and αamylase inhibitory activity were found as 0.45-0.67, and 1.2-1.8 mmol ACAEs/g, respectively. The oral acute toxicity test indicated the safety of 600mg/kg dosage of different parts of A. bruguieri on albino rats without behavioral abnormality or mortality. The current study is considered as the first report on the A. bruguieri as a possible new source of biocompatible material for many industrial products.

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“…ROS stimulate the biosynthesis of various secondary compounds that show anti-oxidative activity ( Zandi and Schnug, 2022 ). Moreover, ROS interfere with intracellular trafficking ( Zwiewka et al., 2015 ; Paponov et al., 2020 ), another important plant process that affects secondary metabolism ( Roze et al., 2011 ) and therefore might also modulate the accumulation of desired pharmaceuticals and phytochemicals. ROS also play a key role in the development of root hairs, which are now viewed as impotant sites of secondary metabolite production ( Dayan and Duke, 2003 ).…”

Section: Introductionmentioning

confidence: 99%

Light exposure of roots in aeroponics enhances the accumulation of phytochemicals in aboveground parts of the medicinal plants Artemisia annua and Hypericum perforatum

2023

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Light acts as a trigger to enhance the accumulation of secondary compounds in the aboveground part of plants; however, whether a similar triggering effect occurs in roots is unclear. Using an aeroponic setup, we investigated the effect of long-term exposure of roots to LED lighting of different wavelengths on the growth and phytochemical composition of two high-value medicinal plants, Artemisia annua and Hypericum perforatum. In A. annua, root exposure to white, blue, and red light enhanced the accumulation of artemisinin in the shoots by 2.3-, 2.5-, and 1.9-fold, respectively. In H. perforatum, root exposure to white, blue, red, and green light enhanced the accumulation of coumaroylquinic acid in leaves by 89, 65, 84, and 74%, respectively. Root lighting also increased flavonol concentrations. In contrast to its effects in the shoots, root illumination did not change phytochemical composition in the roots or root exudates. Thus, root illumination induces a systemic response, resulting in modulation of the phytochemical composition in distal tissues remote from the light exposure site.

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Butylated Hydroxytoluene (BHT) Inhibits PIN1 Exocytosis From BFA Compartments in Arabidopsis Roots

Cited by 5 publications

References 42 publications

Glutathione Enhances Auxin Sensitivity in Arabidopsis Roots

Glutathione Enhances Auxin Sensitivity in Arabidopsis Roots

Phytochemical profile, Antioxidant, Enzyme inhibitory and acute toxicity activity of Astragalus bruguieri

Light exposure of roots in aeroponics enhances the accumulation of phytochemicals in aboveground parts of the medicinal plants Artemisia annua and Hypericum perforatum

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