Butyric acid as sole product from xylose fermentation by a non-solventogenic Clostridium beijerinckii strain under controlled pH and nutritional conditions

Fonseca, Bruna Constante; Bortolucci, Jonatã; Silva, Thalita Marques da; Passos, Vinícius Fabiano dos; Gouvêa, Paula Fagundes de; Dinamarco, Taísa Magnani; Reginatto, Valéria

doi:10.1016/j.biteb.2020.100426

Cited by 13 publications

(13 citation statements)

References 46 publications

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“…Glycerol consumption and 1,3-PD, acetate, and butyrate formation by C. pasteurianum DSMZ 525: 41.1,22.3,and 16.3% and 93,44.5,and 14% in 80,390, and 1,100 mM glycerol, respectively. Low pH could affect glycerol consumption by C. beijerinckii Br21, as observed for xylose fermentation by the same strain (Fonseca et al, 2020). Low pH increases the concentration of non-dissociated organic acids, which are stronger inhibitors than the corresponding ionized species.…”

Section: Effect Of Glycerol Concentration On Fermentation Products Pr...mentioning

confidence: 83%

“…Low pH increases the concentration of non-dissociated organic acids, which are stronger inhibitors than the corresponding ionized species. Total strain Br21 inhibition was observed at 10 mM of total non-dissociated acid (Fonseca et al, 2020). Therefore, the non-dissociated butyric or acetic acid concentration at ∼5 mM during 390 mM glycerol fermentation could contribute to inhibiting Clostridium metabolism.…”

Section: Effect Of Glycerol Concentration On Fermentation Products Pr...mentioning

confidence: 97%

“…Nevertheless, in our laboratory, we have isolated the non-solventogenic C. beijerinckii Br21 strain (Fonseca et al, 2016(Fonseca et al, , 2019, which cannot reabsorb organic acids to produce the solvents. This causes the organic acids, especially butyric acid, to accumulate in the medium, and ABE fermentation does not occur (Fonseca et al, 2020). A nonsolventogenic C. beijerinckii strain has never been studied for 1,3-PD production from glycerol.…”

Section: Introductionmentioning

confidence: 99%

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The Non-solventogenic Clostridium beijerinckii Br21 Produces 1,3-Propanediol From Glycerol With Butyrate as the Main By-Product

Mermejo

Bortolucci

Andrade

et al. 2022

Front. Sustain. Food Syst.

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Ever-increasing biofuel production has raised the supply of glycerol, an abundant waste from ethanolic fermentation and transesterification, for biodiesel production. Glycerol can be a starting material for sustainable production of 1,3-propanediol (1,3 PD), a valued polymer subunit. Here, we compare how Clostridium pasteurianum DSMZ 525, a well-known 1,3-PD-producer, and the non-solventogenic Clostridium beijerinckii Br21 perform during glycerol fermentation. Fermentative assays in 80-, 390-, or 1,100-mM glycerol revealed higher 1,3-PD productivity by DSMZ 525 compared to Br21. The highest 1,3-PD productivities by DSMZ 525 and Br21 were obtained in 390 mM glycerol: 3.01 and 1.70 mM h−1, respectively. Glycerol uptake by the microorganisms differed significantly: C. beijerinckii Br21 consumed 41.1, 22.3, and 16.3%, while C. pasteurianum consumed 93, 44.5, and 14% of the initial glycerol concentration in 80, 390, and 1,100 mM glycerol, respectively. In 1,100 mM glycerol, C. beijerinckii Br21 growth was delayed. Besides 1,3-PD, we detected butyrate and acetate during glycerol fermentation by both strains. However, at 80 mM glycerol, C. beijerinckii Br21 formed only butyrate as the by-product, which could help downstream processing of the 1,3-PD fermentation broth. Therefore, C. beijerinckii Br21, an unexplored biocatalyst so far, can be used to convert glycerol to 1,3-PD and can be applied in biofuel biorefineries.

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Section: Effect Of Glycerol Concentration On Fermentation Products Pr...mentioning

confidence: 83%

Section: Effect Of Glycerol Concentration On Fermentation Products Pr...mentioning

confidence: 97%

Section: Introductionmentioning

confidence: 99%

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The Non-solventogenic Clostridium beijerinckii Br21 Produces 1,3-Propanediol From Glycerol With Butyrate as the Main By-Product

Mermejo

Bortolucci

Andrade

et al. 2022

Front. Sustain. Food Syst.

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“…This is because the acid-base treatment inevitably introduces new impurities into the process of converting sucrose into reducing sugar. Although dilute acid can effectively hydrolyze bagasse, aldehydes, phenols, and other substances unfavorable to cell growth will be produced more or less in the hydrolysis process ( Fonseca et al, 2020 ). After hydrolysis, a large number of sodium ions will be produced in the process of neutralization with sodium hydroxide.…”

Section: Resultsmentioning

confidence: 99%

Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production

Wang

Zhou²,

Liu³

et al. 2023

Front. Microbiol.

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IntroductionClostridium tyrobutyricum has considerable prospect in the production of organic acids. Globally, refinery final molasses is rich in sugar and reported to have high levels of accumulation and high emission costs, recognized as an excellent substrate for C. tyrobutyricum fermentation, but there is no suitable method available at present.MethodsIn this study, an acid-base treatment combined with a new green membrane treatment technology – a dynamic ion-exchange membrane -was used to pretreat refinery final molasses, so that it could be used for C. tyrobutyricum to produce butyric acid. A high-performance liquid chromatography method was established to determine the conversion of a large amount of sucrose into fermentable sugars (71.88 g/L glucose and 38.06 g/L fructose) in the treated refinery final molasses. The process of sequential filtration with 3, 1, and 0.45 μm-pore diameter dynamic ion-exchange membranes could remove impurities, pigments, and harmful substances from the refinery final molasses, and retain the fermentable sugar.Results and discussionThis means that refinery final molasses from the sugar industry could be utilized as a high-value by-product and used for the growth of C. tyrobutyricum, with industrial feasibility and economic competitiveness. Using the treated refinery final molasses as a carbon source, C. tyrobutyricum was screened by the method of adaptive evolution. The strain with butyric acid yielded 52.54 g/L, and the yield of the six carbon sugar was increased from 0.240 to 0.478 g/g. The results showed that combination of C. tyrobutyricum and ionic membrane technology broke through the bottleneck of its utilization of refinery final molasses. This study provided an innovative idea for the C. tyrobutyricum fermentation to produce butyric acid.

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“…Consequentemente há a liberação de H + no meio intracelular, que leva ao colapso do gradiente de pH transmembrana (DÜRRE, 2007;FONSECA et al, 2020;MADDOX et al, 2000).…”

Section: Transformação De E Coli Xl1-blueunclassified

Produção de 1,3-propanodiol por Clostridium beijerinckii Br21 a partir de glicerol: expressão gênica das principais enzimas envolvidas e manipulação genética por transformação

Bortolucci¹

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Ao longo desses anos de Mestrado, sou grato por muitas coisas! Primeiramente gostaria de agradecer aos meus pais, Roseli e Jair, que me possibilitaram a oportunidade aos estudos durante toda minha formação. Desde o ensino fundamental, médio e superior, sempre tive total apoio de vocês: emocional, financeiro, enfim, de todas as formas possíveis. Sou eternamente grato! Em seguida, gostaria de agradecer à minha orientadora, Profa. Dra. Valeria Reginatto Spiller. Foram anos de atuação em seu laboratório, que se iniciaram ainda durante a minha graduação, como Iniciação Científica. E, durante todos esses anos, sempre tive seu apoio, incentivo e confiança em meu trabalho. Agradeço muito por tudo que você sempre fez por mim! Com certeza nada disso seria possível sem sua orientação! Também gostaria de agradecer aos demais integrantes do grupo de pesquisa: Bruna,

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Butyric acid as sole product from xylose fermentation by a non-solventogenic Clostridium beijerinckii strain under controlled pH and nutritional conditions

Cited by 13 publications

References 46 publications

The Non-solventogenic Clostridium beijerinckii Br21 Produces 1,3-Propanediol From Glycerol With Butyrate as the Main By-Product

The Non-solventogenic Clostridium beijerinckii Br21 Produces 1,3-Propanediol From Glycerol With Butyrate as the Main By-Product

Construction of Clostridium tyrobutyricum strain and ionic membrane technology combination pattern for refinery final molasses recovery and butyric acid production

Produção de 1,3-propanodiol por Clostridium beijerinckii Br21 a partir de glicerol: expressão gênica das principais enzimas envolvidas e manipulação genética por transformação

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