Butyric acid fermentation in a fibrous bed bioreactor with immobilized Clostridium tyrobutyricum from cane molasses

Jiang, Ling; Wang, Jufang; Song, Liang; Wang, X.; Chen, Peilin; Xu, Zhinan

doi:10.1016/j.biortech.2009.02.032

Cited by 180 publications

(110 citation statements)

References 31 publications

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“…Prior studies of the fermentative production of FAs used clostridia to produce high levels of butyrate or hexanoate from glucose or galactitol, respectively (Jiang et al, 2010;Wei et al, 2013). However, clostridia are disadvantageous due to slow growth, a requirement for strictly anaerobic culture conditions, sporulation and relatively limited genetic tools for use in strain improvement and product diversification (Baek et al, 2013;Fischer et al, 2010;Lim et al, 2013;Zhang et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

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Fermentative production of short-chain fatty acids in Escherichia coli

et al. 2014

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Escherichia coli was engineered for the production of even-and odd-chain fatty acids (FAs) by fermentation. Co-production of thiolase, hydroxybutyryl-CoA dehydrogenase, crotonase and trans-enoyl-CoA reductase from a synthetic operon allowed the production of butyrate, hexanoate and octanoate. Elimination of native fermentation pathways by genetic deletion (DldhA, DadhE, DackA, Dpta, DfrdC) helped eliminate undesired by-products and increase product yields. Initial butyrate production rates were high (0.7 g l "1 h "1 ) but quickly levelled off and further study suggested this was due to product toxicity and/or acidification of the growth medium. Results also showed that endogenous thioesterases significantly influenced product formation. In particular, deletion of the yciA thioesterase gene substantially increased hexanoate production while decreasing the production of butyrate. E. coli was also engineered to co-produce enzymes for even-chain FA production (described above) together with a coenzyme B 12 -dependent pathway for the production of propionyl-CoA, which allowed the production of odd-chain FAs (pentanoate and heptanoate). The B 12 -dependent pathway used here has the potential to allow the production of odd-chain FAs from a single growth substrate (glucose) in a more energy-efficient manner than the prior methods.

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Section: Discussionmentioning

confidence: 99%

“…Certain bacteria have native fermentation pathways that produce butyrate and hexanoate and have been used for the production of these FAs. Clostridium tyrobutyricum was used to produce high levels of butyric acid from various substrates in a fibrous bed reactor (Jiang et al, 2010;Wei et al, 2013). Clostridium sp.…”

Section: Introductionmentioning

confidence: 99%

Fermentative production of short-chain fatty acids in Escherichia coli

et al. 2014

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“…Besides, the cell immobilization technique in continuous fermentation is a promising method with many advantages over the conventional suspended cell fermentation process. The immobilized cells can maintain high viable cell densities in a reactor and eliminates the cellular lag phase to increase the productivity due to the possibility of sustaining higher dilution rates [18]. Moreover, the immobilized cell reactors might enhance the tolerance to adverse conditions such as butanol toxicity [19,20].…”

Section: Introductionmentioning

confidence: 99%

Enhanced isopropanol–butanol–ethanol (IBE) production in immobilized column reactor using modified Clostridium acetobutylicum DSM792

Bankar

Jurgens

Survase

et al. 2014

Fuel

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Highlights Metabolic engineering to convert acetone into isopropanol  Continuous immobilized fermentation to validate industrial feasibility of strain  Optimization of bioprocess to enhance the solvent yield and productivity  Utilization of lignocellulosic spent liquor as a substrate for the fermentation AbstractThe co-production of acetone during traditional butanol fermentation (acetone-butanol-ethanol) lowers the yield of alcohol biofuels. The present study was aimed to develop an improved Clostridium acetobutylicum strain with enhanced alcohol fuel production capability. The previously developed IBE producing C. acetobutylicum DSM792-ADH strain was further optimized to enhance the solvent productivity and yield. Immobilized cell column reactor was used to investigate the effect of dilution rate on IBE fermentation. Pure glucose, artificial sugar mixture and SEW spent liquor from spruce chips were used as substrates to study the behavior of the modified microorganism. The highest total solvent concentrations of approximately10.60 g.l -1 , 10 g.l -1 and 6 g.l -1 were obtained when glucose, sugar mixture and SEW spent liquor were used as substrate respectively. The modified microorganism could effectively utilize the lignocellulosic biomass hydrolyzate (SEW spent liquor) to yield a solvent productivity of 1.67 g.l -1 .h -1 .

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“…The production of acetate and lactate increased as the pH was decreased to 6.0, and lactate and acetate were the main products when the pH was below 5.0. In the study by Jiang et al (2009), a fibrous bed bioreactor with immobilized C. tyrobutyricum was used on cane molasses to produce butyric, and pH 6.0 was found optimum. Some researchers focused on the effect of initial sugar concentration on fermentation by C. tyrobutyricum.…”

Section: Introductionmentioning

confidence: 99%