2003
DOI: 10.1038/sj.bjp.0705010
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Butyrylcholinesterase and acetylcholinesterase activity and quantal transmitter release at normal and acetylcholinesterase knockout mouse neuromuscular junctions

Abstract: 1 The present study was performed to evaluate the presence and the physiological consequences of butyrylcholinesterase (BChE) inhibition on isolated phrenic-hemidiaphragm preparations from normal mice expressing acetylcholinesterase (AChE) and BChE, and from AChE-knockout mice (AChE 7/7 ) expressing only BChE. 2 Histochemical and enzymatic assays revealed abundance of AChE and BChE in normal mature neuromuscular junctions (NMJs). 3 In normal NMJs, in which release was reduced by low Ca 2+ /high Mg 2+ medium BC… Show more

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Cited by 78 publications
(66 citation statements)
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“…These results show that the presynaptic modulation of quantal transmitter release depends on the activity of BChE. (90) One hypothesis is that the role of BChE is to quench the thirst for choline.…”
Section: Mousementioning
confidence: 77%
“…These results show that the presynaptic modulation of quantal transmitter release depends on the activity of BChE. (90) One hypothesis is that the role of BChE is to quench the thirst for choline.…”
Section: Mousementioning
confidence: 77%
“…Hemidiaphragm muscles with their associated phrenic nerves were isolated, and electrophysiological recordings were done according to Minic at al. (24). Evoked endplate potentials (EPPs) and/or spontaneous miniature endplate potentials (MEPPs) were recorded with intracellular microelectrodes filled with 3 M KCl solution (8-12 MΩ resistance) using conventional techniques (24).…”
Section: Methodsmentioning
confidence: 99%
“…(24). Evoked endplate potentials (EPPs) and/or spontaneous miniature endplate potentials (MEPPs) were recorded with intracellular microelectrodes filled with 3 M KCl solution (8-12 MΩ resistance) using conventional techniques (24). The motor nerve was stimulated via a suction electrode with current pulses of 0.1-ms duration and supramaximal voltage (typically 3-8 V) at different stimulating frequencies.…”
Section: Methodsmentioning
confidence: 99%
“…Moreover, neuromuscular transmission in the soleus from WT mice is enhanced by LT, via a reduced rundown of endplate potential during high-frequency continuous nerve stimulation, 34 an event that is exaggerated in AChE-deficient mice. 36 It would be of interest in the future to confirm the effect of LT using another animal model of perturbed neurotransmission.…”
Section: Beneficial Effect Of Lt On Fatigue Resistance In Thementioning
confidence: 99%