Butyrylcholinesterase – Positive Innervation of the Spleen in Rats

Abstract

Innervation of the spleen in rats was studied. Butyrylcholinesterase-(BuChE)-positive nerve components of the organ were visualized by the direct thiocholine method. BuChE-positive nerve components enter the spleen in a common bundle with arteries. In the organ they form characteristic periarterial and periarteriolar plexiform arrangements, which are especially conspicuous around the aa. centrales running through the white pulp. Then, nerve fibres extend away from these plexuses into adjacent layers of trabeculae further into marginal layers of periarterial lymphatic sheath (PALS) as well as into the mantle zone of follicles. Several scattered periarteriolar and solitary nerve fibres can be seen in the marginal sinuses and cords of the red pulp. In the fibrous capsula BuChE-positive nerve fibres can also be seen which have an evident connection with trabecular and parenchymal nerves of the organ. Microscopic findings support the notion that BuChE-positive nerve profiles supply not only the vasculature, but also the parenchymal components of the spleen, and they may participate, to a great extent, in the regulation of the immune processes in this organ.

The diffuse reduction in spleen density: an indicator of severe acute pancreatitis?

“…We will study this issue in future studies under the informed consent of patients. Furthermore, Sirotakova et al [ 51 ] showed that the splenic sympathetic nerve, accompanied by the splenic artery entering the spleen, regulates spleen haemoperfusion and the immune function of the spleen lymphocytes; MacNeil BJ et al [ 52 ] found that the relationship between the electrophysiological activity of the spleen and endotoxins occurred in a dose-dependent manner. That is, with more endotoxins, we could see higher activity intensity of the spleen sympathetic nerve, a shorter incubation period and less haemoperfusion of the spleen.…”