C 02 Phenotype and functional profile of T cells expressing TCRγδ receptor from patients with active Behçet's disease (BD)

Hamzaoui, K; Hentati, Fayçal; Hamzaoui, A.; Chabbou, A.; Hamida, M. Ben; Ayed, K

doi:10.1016/s0248-8663(05)82249-0

Cited by 27 publications

(34 citation statements)

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“…In addition to ab þ T cells, gd þ T cells in BD are of increasing interest. Significantly increased levels of gd þ T cells in BD, as demonstrated in the present study, are in agreement with other reports [14][15][16]. Proliferation of gd þ T cells in response to hsp-derived peptides in BD patients suggests gd þ T cell involvement in BD [17].…”

supporting

confidence: 93%

“…Immunohistological studies of involved tissues such as oral ulcers, ocular lesions, erythema nodosum-like lesions, skin pathergy reaction and lesions of terminal ileum reveal a predominant T cell infiltration [11][12][13]. Besides ab þ T cells, increases in the gd þ T cell populations in peripheral blood and in inflammatory sites of BD patients have also been reported by several groups [14][15][16]. Moreover, gd þ T lymphocyte responses to particular hsp-derived peptides have been demonstrated to correlate with disease activity in BD patients [17].…”

Section: Introductionmentioning

confidence: 78%

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Peripheral blood T cell expansions in patients with Behc¸et's disease

Esin

̈L

Hodara

et al. 1997

Clinical and Experimental Immunology

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SUMMARYBehçet's disease (BD) is a chronic multisystemic inflammatory disorder characterized mainly by recurrent oral and genital aphthous ulcerations and uveitis. Etiology and pathogenesis of BD remain unknown. T cell receptor (TCR) Va/Vb gene product expression as well as Jb gene segment expression in peripheral blood of BD patients were analysed to investigate the possible role of T lymphocytes in the etiopathogenesis of BD. Flow cytometry with 12 TCR V-specific MoAbs was used for TCRV analyses. Jb gene segment usage by T cell populations expressing certain Vbs was determined by polymerase chain reaction (PCR) technique with Vb-and Cb-specific primers, Southern blotting of PCR products, and subsequent hybridization with radiolabelled Jb gene segment-specific probes. Although 13 of the 23 BD patients exhibited increases in expression of one or more TCR V-gene products, only expansions among the CD4 þ T cell subset were significantly more frequent in BD patients (7/23) compared with healthy controls (0/15) (P ¼ 0 . 019). Six out of eight cases followed for up to 20 months had at least one expansion correlated with disease activity. A strict preference for particular Jb gene segments implicating clonality was apparent in all analysed T cell expansions and correlated well with disease activity. These results suggest a possible involvement of antigen-specific T lymphocytes in the pathogenesis of BD.

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supporting

confidence: 93%

Section: Introductionmentioning

confidence: 78%

Peripheral blood T cell expansions in patients with Behc¸et's disease

Esin

̈L

Hodara

et al. 1997

Clinical and Experimental Immunology

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“…T and B cell responses to peptides derived from mycobacterial and human 65-kD heat shock protein (hsp) were also shown in BD [9][10][11][12]. Increases in gd þ T cells in peripheral blood and cerebrospinal fluid and gd þ T cell responses to hsp-derived peptides also suggest a role for this T cell subset in the aetiopathogenesis of BD [13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Oligoclonal T cell expansions in patients with Behçet's disease

Direşkeneli

Ekşioğlu-Demiralp²,

Kibaroglu³

et al. 1999

Clinical and Experimental Immunology

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SUMMARYBehçet's disease (BD) is a multisystem disorder with oral and genital ulcers, mucocutaneous, ocular, joint, vascular and central nervous system involvement. In this study, the peripheral T cell repertoire was analysed in patients with BD with MoAbs against T cell receptor (TCR) Vb gene products in CD4 þ and CD8 þ T cell compartments, and these were compared with rheumatoid arthritis (RA) patients and healthy controls (HC). In the CD4 þ T cell compartment, oligoclonal TCR Vb expression was observed in 56% of BD (10/18), 71% of RA (5/7) patients and 21% (3/14) of HC. In the CD8 þ T cell group 50% of BD (9/18), 57% of RA patients and 28% of HC (4/14) had an oligoclonal TCR repertoire. An increase of TCR Vb5.1 subset was observed in five BD patients among CD8 þ T cells. Other elevations of TCR Vb subsets were heterogeneously distributed with one to three different Vb subsets. Our results suggest an antigen-driven oligoclonal increase of T cells in BD. There was no overall increase in any Vb group to suggest a superantigen effect. Analysis of the responsible antigens causing the increase in T cell subsets may give insights into the aetiopathogenesis of BD and immunomodulation of these T cells may lead to new treatments.

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“…The mean age at onset is the third decade, children are rarely affected, and few neonatal cases have been reported. In a recent study, an increased number of ␥/␦ T cells in the peripheral blood and the involved tissues, and a phenotypically distinct subset of ␥/␦ T cells at sites of inflammation, were reported (2)(3)(4). Furthermore, significant ␥/␦ T cell proliferative responses to mycobacterial 65-kd heat shock protein (HSP) peptides and their homologous peptides derived from the human 60-kd HSP were observed in Behçet's disease patients (5,6).…”

mentioning

confidence: 99%