c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae

Floyd, Kyle A.; Lee, Calvin K.; Xian, Wujing; Nametalla, Mahmoud; Valentine, Aneesa; Crair, Benjamin; Zhu, S. J.; Hughes, Hannah Q.; Chlebek, Jennifer L.; Wu, Daniel C.; Park, Jin Hwan; Farhat, Ali M.; Lomba, Charles J; Ellison, Courtney K.; Brun, Yves V.; Campos-Gómez, Javier; Dalia, Ankur B.; Li, Jun; Biais, Nicolas; Wong, Gerard C. L.; Yildiz, Fitnat H.

doi:10.1038/s41467-020-15331-8

Cited by 78 publications

(88 citation statements)

References 76 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Increased c-di-GMP levels lead to binding of c-di-GMP to FlrA, and inhibition of its activity which in turn diminishes flagellar gene expression (Srivastava et al, 2013). The dynamic extension and retraction of the MSHA pilus (Jones et al, 2015;Wang et al, 2016) is regulated by c-di-GMP via interaction with the ATPase MshE (Floyd et al, 2020). The role of c-di-GMP in the regulation of large adhesins which control reversible cell attachment during biofilm formation also highlights the essentiality of the molecule (Kitts et al, 2019).…”

Section: Regulation Of Biofilm Formation In V Cholerae and Pathogenesismentioning

confidence: 99%

Diguanylate Cyclases in Vibrio cholerae: Essential Regulators of Lifestyle Switching

Biswas

Chouhan

Bandekar

2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

Biofilm formation in Vibrio cholerae empowers the bacteria to lead a dual lifestyle and enhances its infectivity. While the formation and dispersal of the biofilm involves multiple components-both proteinaceous and non-proteinaceous, the key to the regulatory control lies with the ubiquitous secondary signaling molecule, cyclic-di-GMP (c-di-GMP). A number of different cellular components may interact with c-di-GMP, but the onus of synthesis of this molecule lies with a class of enzymes known as diguanylate cyclases (DGCs). DGC activity is generally associated with proteins possessing a GGDEF domain, ubiquitously present across all bacterial systems. V. cholerae is also endowed with multiple DGCs and information about some of them have been pouring in over the past decade. This review summarizes the DGCs confirmed till date in V. cholerae, and emphasizes the importance of DGCs and their product, c-di-GMP in the virulence and lifecycle of the bacteria.

show abstract

Section: Regulation Of Biofilm Formation In V Cholerae and Pathogenesismentioning

confidence: 99%

Diguanylate Cyclases in Vibrio cholerae: Essential Regulators of Lifestyle Switching

Biswas

Chouhan

Bandekar

2020

Front. Cell. Infect. Microbiol.

View full text Add to dashboard Cite

show abstract

“…All serotype O1 strains contained the autotransporter adhesion ( TAA ) gene ( Rock & Nelson, 2006 ) and Mannose-sensitive haemagglutinin biogenesis protein MshQ gene ( Floyd et al, 2020 ); none of these genes were found in the serotype O2 or O3 strains. Also, all O1 strains contained the lipase family protein gene ( Rock & Nelson, 2006 ), the heme receptor huvA gene ( Li & Ma, 2017 ), and the toxA gene ( Reyes-Becerril et al, 2016 ); these genes were found in 8%, 71% and 52% of the O2 and O3 strains, respectively.…”

Section: Resultsmentioning

confidence: 99%

Analysis of 44 Vibrio anguillarum genomes reveals high genetic diversity

Hansen

Kudirkienė

Dalsgaard

2020

PeerJ

View full text Add to dashboard Cite

Vibriosis, a hemorrhagic septicemic disease caused by the bacterium Vibrio anguillarum, is an important bacterial infection in Danish sea-reared rainbow trout. Despite of vaccination, outbreaks still occur, likely because the vaccine is based on V. anguillarum strains from abroad/other hosts than rainbow trout. Information about the genetic diversity of V. anguillarum specifically in Danish rainbow trout, is required to investigate this claim. Consequently, the aim of the present investigation was to sequence and to characterize a collection of 44 V. anguillarum strains obtained primarily from vibriosis outbreaks in Danish rainbow trout. The strains were sequenced, de novo assembled, and the genomes examined for the presence of plasmids, virulence, and acquired antibiotic resistance genes. To investigate the phylogeny, single nucleotide polymorphisms were identified, and the pan-genome was calculated. All strains carried tet(34) encoding tetracycline resistance, and 36 strains also contained qnrVC6 for increased fluoroquinolone/quinolone resistance. But interestingly, all strains were phenotypic sensitive to both oxytetracycline and oxolinic acid. Almost all serotype O1 strains contained a pJM1-like plasmid and nine serotype O2A strains carried the plasmid p15. The distribution of virulence genes was rather similar across the strains, although evident variance among serotypes was observed. Most significant, almost all serotype O2 and O3 strains, as well as the serotype O1 strain without a pJM1-like plasmid, carried genes encoding piscibactin biosynthesis. Hence supporting the hypothesis, that piscibactin plays a crucial role in virulence for pathogenic strains lacking the anguibactin system. The phylogenetic analysis and pan-genome calculations revealed great diversity within V. anguillarum. Serotype O1 strains were in general very similar, whereas considerable variation was found among serotype O2A strains. The great diversity within the V. anguillarum serotype O2A genomes is most likely the reason why vaccines provide good protection from some strains, but not from others. Hopefully, the new genomic data and knowledge provided in this study might help develop an optimized vaccine against V. anguillarum in the future to reduce the use of antibiotics, minimize economic losses and improve the welfare of the fish.

show abstract

“…This suggests that for cells demonstrating tethered motion, a progressive twisting of the surface-attached pilus fiber during the circular motion of cells may gradually cause the circular motion to stop by pulling the cell body ever closer to the surface. Although twitching has not be observed in V. cholerae , this is one mechanism by which retraction-like dynamics may be achieved ( Charles et al, 2019 ), possibly in tandem with actual retraction of MSHA pili, which has been shown recently in a different strain of V. cholera ( Floyd et al, 2020 ). Under our conditions, we have not observed MSHA pili retraction events nor have we seen bacterial cells that gradually acquire fluorescence when only maleimide dyes were used.…”

Section: Discussionmentioning

confidence: 97%

Crash landing ofVibrio choleraeby MSHA pili-assisted braking and anchoring in a viscoelastic environment

Zhang

Luo

Feng

et al. 2020

Preprint

View full text Add to dashboard Cite

AbstractMannose-sensitive hemagglutinin (MSHA) pili and flagellum are critical for the surface attachment of Vibrio cholerae. However, the cell landing mechanism remains largely unknown. Here, combining the cysteine-substitution-based labelling method with single-cell tracking techniques, we quantitatively characterized the landing of V. cholerae by directly observing both pili and flagellum of cells in viscous solutions. MSHA pili are evenly distributed along the cell length and can stick to surfaces at any point along the filament. With such properties, MSHA pili are observed to act as a brake and anchor during cell landing which include three phases: running, lingering, and attaching. Resistive-force-theory based models are proposed to describe near-surface motion. Importantly, the role of MSHA pili during cell landing is more apparent in viscous solutions. Our work provides a detailed picture of the landing dynamics of V. cholerae under viscous conditions, which can provide insights into ways to better control V. cholerae infections.

show abstract

c-di-GMP modulates type IV MSHA pilus retraction and surface attachment in Vibrio cholerae

Cited by 78 publications

References 76 publications

Diguanylate Cyclases in Vibrio cholerae: Essential Regulators of Lifestyle Switching

Diguanylate Cyclases in Vibrio cholerae: Essential Regulators of Lifestyle Switching

Analysis of 44 Vibrio anguillarum genomes reveals high genetic diversity

Crash landing ofVibrio choleraeby MSHA pili-assisted braking and anchoring in a viscoelastic environment

Contact Info

Product

Resources

About