[C. J. Irving letter to Henry James Murton and Audit Office]

Irving, Catherine; Murton, Henry James.

doi:10.5962/bhl.title.153455

Cited by 16 publications

(23 citation statements)

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“…Samples were analyzed for metabolites of N-2-fluorenylacetamide and N-2-fluorenylacethydroxamic acid by methods previously used (Irving, 1962). Thin layer chromatography of glucuronide conjugates was carried out on cellulose (MN 300 F2~4, Brinkmann Instruments, Inc., Westbury, N. Y . )…”

Section: Experimental and Resultsmentioning

confidence: 99%

“…The rate of alkaline hydrolysis of the glucuronide in 0.01 M NaOH in 50z ethanol was determined by measuring the disappearance of absorbance at 302 mp upon addition of the alkali to a solution of the glucuronide. The concentration of glucuronide at time ( i ) was calculated (Irving, 1960) from the molar extinction coefficients of the glucuronide and the product at 302 mp. Results of the kinetic studies of the alkaline degradation of sodium (N-acetyl-N-2-fluorenylhydroxylamine 0-D-gIucosid)uronate ( k = 10.9 X lop4 sec-I) are shown in Figure 4.…”

Section: Isolation Of Sodium (N-acetyl-n-2-fluorenylh~~droxyl-mentioning

confidence: 99%

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Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid^*

Hill¹,

Irving²

1967

Biochemistry

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Section: Experimental and Resultsmentioning

confidence: 99%

Section: Isolation Of Sodium (N-acetyl-n-2-fluorenylh~~droxyl-mentioning

confidence: 99%

Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid^*

Hill¹,

Irving²

1967

Biochemistry

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“…Thus, the higher microhid colonization o f the rat stomach, especially of the forestomach (KUNSTYR 1974), in comparison with the pig (SCHULTZE and BATHKE 1977) seems to be ofgreat importance for the ability of the rat to better hydrolyze phytate. For optimal bacterial phytase activities p H values between 4 and 6 were reported (IRVING 1980).…”

Section: Wheatmentioning

confidence: 99%

Comparative Study of Phosphorus Utilization from Wheat, Barley and Corn Diets by Young Rats and Pigs¹

Lantzsch

Hillenbrand

Scheuermann

et al. 1992

Animal Physiology Nutrition

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Zusammenfassung Vergleichende Untersuchung an Ratten und Schweinen zur Verwertung von Phosphor aus Weizen‐, Gerste‐ und Maisdiäten In einem 3‐wöchigen Versuch mit 36 wachsenden männlichen Ratten (97 g) und in einem 4‐wöchigen Versuch mit 18 kastrierten wachsenden männlichen Schweinen (16 kg) wurde die Fähigkeit berder Spezies zur Verwertung von Phosphoraus Weizen‐, Gerste‐ und Maisdiäten verglichen. Die Versuchsdiaten enthielten dieselben Gehalte an Calcium (0.51–0.52%), Phytat‐(0.14–0.20%) und Gesamipaosphot (0.33 0.15%), letzteren auf einem marginalen Niveau. Während die Ratten der, Gesamtphosphor aller drei Getreidediäten in vergleichbarer Höhe absorbierten und verwerteten, traten bei den Schweinen signifikante Unterschiede auf. Die Tiere der Weizengruppe absorbierten den Phosphor gleich gut wie die Ratten. wiesen aber eine geringere Verwertung auf. In den Gerste‐ und Maisgruppen wurde der Phosphor signifikant schlechter absorbiert und verwertet Die bessere Absorption des Getreidephosphors bei den Ratten ist im Wesentlichen auf die bessere Fähigkeit dieser Tiere zur Phytatspaltung in Magen und Dünndarm zurückzuführen. Die Ergebnisse werden im Zusammenhang mit den höheren pH‐Werten im Magen und der langsameren Futterpassage im Magen und Dünndarm der Ratten, sowie anderen aus der Literatur bekannten Aspekten diskutiert.

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“…Sedimentation rates of single-strand DNA from carcinogen-treated and carcinogen-free permeabilized cells were determined in a Beckman L 2-65 preparative Car c inogens N-Acetoxy-N-2-acetylaminofluorene, abbreviated (Ac),ONFln, was synthesized by reduction of 2nitrofluorene [39], and acetylation of the resulting N-hydroxy-2-amino-fluorene with acetic anhydride [40] to yield (Ac),ONFln. Purification was achieved by converting this product to N-hydroxy-2-acetylamino-fluorene (1 2.5 % aqueous NH3 solution), crystallization (twice) of the latter from benzene and reacetylation with acetic anhydride [41].…”

Section: Sedimentation Analysismentioning

confidence: 99%

Carcinogen‐Induced DNA Repair in Nucleotide‐Permeable Escherichia coli Cells

Thielmann

1976

European Journal of Biochemistry

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Upon exposure to the carcinogens N-acetoxy-N-2-acetylaminofluorene and 7-bromomethylbenz [a]anthracene, which bind covalently to DNA, ether-permeabilized (nucleotide-permeable)Esclzerichia coli wild-type cells responded with DNA excision repair. This repair was missing in mutants carrying defects in genes uvrA, uvrB and uvrC, whereas it was present in uvrD and several rec mutants.Enzymic activities involved were identified by measuring repair polymerization and size reduction of denatured DNA.1. An easily measurable effect in E. coli wild-type cells was carcinogen-induced repair polymerization. When initiated by N-acetoxy-N-2-acetylaminofluorene or 7-bromomethyl-benz[u]anthrdcene, it depended upon an ATP-requiring step; CTP, GTP or UTP did not substitute for ATP. DNA repair synthesis was inhibited by p-chloromercuribenzoate and quinacrine. In uvrA, uvrB and uvrC mutants no carcinogen-stimulated DNA synthesis could be detected, indicating that steps involved in pyrimidine dimer excision are also involved in chemorepair. In r e d , recB and recC mutant cells, repair synthesis was stimulated by the carcinogens to a normal extent. This evidence excludes the ATP-dependent recB,C deoxyribonuclease and recA gene products as playing an important role in carcinogen-induced excision repair. polA, cells showed drastically reduced levels of repair polymerization, indicating that DNA polymerase I is the main polymerizing enzyme.2. As determined by DNA size reduction in alkaline sucrose gradients, the arylalkylating carcinogens caused endonucleolytic cleavage of endogenous DNA in wild-type cells. This incision step was most effectively performed in the presence of ATP; UTP, CTP and GTP were only slightly effective. Incision was inhibited by p-chloromercuribenzoate and quinacrine. When exposed to the arylalkylating carcinogens, uvrA, uvrB and uvrC mutant cells did not perform the incision step in the presence of ATP, suggesting the involvement of the respective gene products in the initiation of chemorepair.Several potent chemical carcinogens are known to be able to bind covalently to the genomes of living

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[C. J. Irving letter to Henry James Murton and Audit Office]

Cited by 16 publications

References 0 publications

Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid^*

Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid^*

Comparative Study of Phosphorus Utilization from Wheat, Barley and Corn Diets by Young Rats and Pigs¹

Carcinogen‐Induced DNA Repair in Nucleotide‐Permeable Escherichia coli Cells

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[C. J. Irving letter to Henry James Murton and Audit Office]

Cited by 16 publications

References 0 publications

Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid*

Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid*

Comparative Study of Phosphorus Utilization from Wheat, Barley and Corn Diets by Young Rats and Pigs1

Carcinogen‐Induced DNA Repair in Nucleotide‐Permeable Escherichia coli Cells

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Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid^*

Biosynthesis and Studies of the Alkaline Sensitivity of the NO-Glucuronide of the Carcinogen N-2-Fluorenylacethydroxamic Acid^*

Comparative Study of Phosphorus Utilization from Wheat, Barley and Corn Diets by Young Rats and Pigs¹