c-Jun inhibits NF-E2 transcriptional activity in association with p18/maf in Friend erythroleukemia cells

cAMP-induced NF-κB (p50/relB) binding to a c-myb intronic enhancer correlates with c-myb up-regulation and inhibition of erythroleukemia cell differentiation

“…`Supershift' assays showed that the AP-1/DNA complex induced by cAMP contained JunB, JunD and c-Fos protein (Figure 8c). Like previous workers, we found that MEL cells do not express detectable levels of c-Jun protein (Francastel et al, 1994).…”

Involvement of c-JUN in the regulation of terminal differentiation genes in normal and malignant keratinocytes

“…These data strongly suggest that c-JUN is a general inhibitor of keratinocyte terminal di erentiation. A similar inverse relation between c-jun expression and di erentiation ability has also been described in a murine erythroleukemia cell line (Francastel et al, 1994) suggesting that the phenomenon might be even more general (see also promoter activity during calcium induced di erentiation in normal human keratinocytes. Sprr2a promoter activity was determined by measuring the CAT activity from either plasmid pSG122 which contains a 1500 bp promoter fragment (bars 1 ± 5) or from plasmid pSG136 which contains the 134 bp minimal sprr2a promoter (bars 6 and 7).…”

NF-E2p18/mafK is required in DMSO-induced differentiation of Friend erythroleukemia cells by enhancing NF-E2 activity

“…For example, we have previously reported that the presence of c-jun expression in TFP10 cells can decrease their ability to differentiate by the DMSO. 31 The smaller p18 subunit of NF-E2 was described for the first time in F-MEL cells. It was reported that NF-E2 p18 is coded by the mafK gene which belongs to the small Maf family proteins lacking transactivation domains.…”

“…viously described. 30,31 For Northern blot analysis, the following hybridization probes were used: p18, a 0.51-kb EcoRI fragment of cDNA NF-E2 p18; p45, a 1.3-kb EcoRI fragment of cDNA NF-E2p45; ␣ globin, a 1.5-kb PstI fragment human Transient transfection experiments genomic fragment; GAPDH, a 1.3-kb PstI fragment of cDNA of rat GAPDH. For RT-PCR analysis of p18 sense and antiTranscriptional activation assays were performed by transient transfections of F-MEL or NIH/3T3 cells with a hGH reporter sense expression, the upstream primer is located in the T7 RNA promoter of the pcDNA3 vector (position 862-882).…”