C‐reactive protein and high mobility group box 1 in dogs with gastric dilatation and volvulus

Uhríková, I.; Raušerová-Lexmaulová, Leona; Řeháková, Kristína; Scheer, Peter; Doubek, Jaroslav

doi:10.1111/vec.12324

Cited by 14 publications

(28 citation statements)

References 24 publications

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“…Several studies have measured blood HMGB‐1 concentrations in dogs . In dogs with GDV, high HMGB‐1 concentrations were associated with gastric necrosis and with nonsurvival . In that study, the kinetics of HMGB‐1 were distinct from those of C‐reactive protein, in that HMGB‐1 concentrations did not significantly increase after surgery, although dogs were only followed to 22 hours post‐surgery.…”

Section: Discussionmentioning

confidence: 92%

“…Several studies have measured blood HMGB‐1 concentrations in dogs . In dogs with GDV, high HMGB‐1 concentrations were associated with gastric necrosis and with nonsurvival .…”

Section: Discussionmentioning

confidence: 99%

“…Concentrations of plasma HMGB‐1 were analyzed using a commercial ELISA, as previously reported , . An antibody‐coated magnetic microsphere‐based multiplex cytokine immunoassay kit designed for the simultaneous quantification of 13 cytokines was used to measure cytokines as previously described , .…”

Section: Methodsmentioning

confidence: 99%

“…A related DAMP is high mobility group box‐1 (HMGB‐1), a DNA‐binding nuclear protein and transcription regulator that, in the extracellular environment, stimulates the innate immune response via TLR2 and TLR4 . Increased concentrations of HMGB‐1 are associated with poor outcome in human sepsis, are increased in dogs with gastric‐dilation‐volvulus (GDV), and correlate with plasma nucleosome concentrations in critically ill horses, suggesting it may provide valuable information in dogs with sepsis.…”

Section: Introductionmentioning

confidence: 99%

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Evaluation of the host cytokine response in dogs with sepsis and noninfectious systemic inflammatory response syndrome

Goggs

Letendre

2019

J Vet Emergen Crit Care

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Objective: To quantify plasma cytokine concentrations in dogs with sepsis and noninfectious systemic inflammation and to evaluate the association between plasma cytokines and outcome in dogs with sepsis.Design: Prospective, observational cohort study. Setting: University teaching hospital.Animals: Forty-five dogs with sepsis, 10 dogs with noninfectious systemic inflammation (nSIRS), and 15 healthy controls were consecutively enrolled from June 2015 to February 2016 and followed to hospital discharge. Dogs with sepsis satisfied ≥2 SIRS criteria and had a documented or highly suspected bacterial infection. Dogs with nSIRS satisfied ≥2 SIRS criteria but had no evidence of infection. Dogs <3 kg and those with documented coagulopathy were excluded.Interventions: Measurement of inflammatory cytokines and high-mobility group box-1 (HMGB-1) was performed on each group. Measurements and Main Results:High-mobility group box-1 concentrations were analyzed by ELISA. Plasma concentrations of 13 cytokines were measured in singlet using multiplex magnetic bead assays. Kruskal-Wallis with Dunn's multiple comparison tests were used to compare biomarker concentrations between groups. Mann-Whitney U-tests were used to compare biomarker concentrations between survivors and nonsurvivors. Associations between biomarkers were evaluated using Spearman's correlation coefficients. Independent outcome predictors were identified using multivariable logistic regression. Alpha was set at 0.05. Concentrations of interleukin (IL)-6, C-X-C motif chemokine (CXCL)-8, keratinocyte-derived chemokine (KC)-like, C-C motif chemokine ligand 2 (CCL2), and HMGB-1 were significantly greater in dogs with sepsis versus healthy controls (all P ≤ 0.034). In dogs with sepsis, only CCL2 was independently associated with survival (odds ratio [OR] 0.996, 95% CI 0.993-0.999, P = 0.004). A cut-off of 385 pg/mL for CCL2 was 80% sensitive and 91.4% specific for nonsurvival (area under the ROC curve [AUROC] 0.866). Conclusions:Dogs with sepsis have significantly increased concentrations of HMGB-1 and inflammatory cytokines, including IL-6, CXCL8, and KC-like. Increased CCL2 concentration is a negative prognostic indicator in dogs with sepsis. These findings should be confirmed using duplicate analyses in larger, distinct populations of dogs with sepsis before applying them to clinical patients. K E Y W O R D Scanine, CCL2, HMGB-1, outcome, SIRS

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Section: Discussionmentioning

confidence: 92%

“…Several studies have measured blood HMGB‐1 concentrations in dogs . In dogs with GDV, high HMGB‐1 concentrations were associated with gastric necrosis and with nonsurvival .…”

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Evaluation of the host cytokine response in dogs with sepsis and noninfectious systemic inflammatory response syndrome

Goggs

Letendre

2019

J Vet Emergen Crit Care

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“…Dogs with GDV frequently fulfill the criteria for the presence of systemic inflammatory response syndrome (SIRS) ( 3 ). Tissue hypoperfusion, cellular necrosis, and ischemia-reperfusion injury are the main triggers of SIRS in the perioperative period ( 3 , 4 ), and increased plasma concentrations of SIRS-associated biomarkers, such as C-reactive protein and high-mobility group box-1 (HMGB1) have been reported in GDV dogs ( 4 , 5 ). Blood lactate concentrations have been demonstrated to be prognostic in GDV syndrome ( 1 ).…”

Section: Introductionmentioning

confidence: 99%

Cell-Free DNA, High-Mobility Group Box-1, and Procalcitonin Concentrations in Dogs With Gastric Dilatation–Volvulus Syndrome

et al. 2018

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Canine gastric dilatation–volvulus (GDV) is a life-threatening disease characterized by extensive tissue ischemia, tissue hypoperfusion, and systemic inflammation. Biomarkers that better reflect the severity of gastric necrosis and systemic inflammation would aid clinicians in the management of these patients. This study aimed to investigate the prognostic significance of cell-free DNA (cfDNA), high-mobility group box-1 (HMGB1), and procalcitonin (PCT) in dogs with GDV. Concentrations of cfDNA, HMGB1, and PCT were measured in citrated plasma samples collected from 29 dogs with GDV at hospital admission. Additional data collected included baseline lactate concentrations, APPLEfast score, evidence of gastric necrosis, occurrence of postoperative complications, and outcome. Twenty-four healthy dogs were sampled as controls. Continuous variables between groups were compared with the Mann–Whitney U and correlations between continuous variables were assessed by calculation of Spearman’s correlation coefficient. Alpha was set at 0.05. Dogs with GDV had significantly greater concentrations of cfDNA, HMGB1, and PCT compared to controls (P = 0.0009, P = 0.004, and P = 0.009, respectively). PCT concentrations were significantly higher in non-survivors compared to survivors (P = 0.008). Dogs with gastric necrosis had significantly greater lactate concentrations compared to dogs without gastric necrosis (P = 0.0005). The APPLEfast score was not prognostic. Lactate and PCT concentrations were moderately, positively correlated (rs 0.51, P = 0.0005). Concentrations of the inflammatory biomarkers cfDNA, HMGB1, and PCT are increased in canine GDV. Only lactate and PCT concentrations were prognostic in this population of GDV dogs and were predictive of the presence of gastric necrosis and of non-survival to hospital discharge, respectively.

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Gastric Dilatation Volvulus

Mazzaferro¹,

Monnet²

2023

Small Animal Soft Tissue Surgery

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C‐reactive protein and high mobility group box 1 in dogs with gastric dilatation and volvulus

Abstract: HMGB1 and CRP behaved differently in regards to their kinetic patterns, with HMGB1 appearing to better reflect the severity of tissue injury in dogs with GDV than CRP.

Cited by 14 publications

References 24 publications

Evaluation of the host cytokine response in dogs with sepsis and noninfectious systemic inflammatory response syndrome

Evaluation of the host cytokine response in dogs with sepsis and noninfectious systemic inflammatory response syndrome

Cell-Free DNA, High-Mobility Group Box-1, and Procalcitonin Concentrations in Dogs With Gastric Dilatation–Volvulus Syndrome

Gastric Dilatation Volvulus

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