Cell-Free DNA, High-Mobility Group Box-1, and Procalcitonin Concentrations in Dogs With Gastric Dilatation–Volvulus Syndrome

Troìa, Roberta; Giunti, Massimo; Calipa, Stefano; Goggs, Robert

doi:10.3389/fvets.2018.00067

Cited by 19 publications

(25 citation statements)

References 41 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The final diagnoses for most dogs were not known at the time of recruitment and hence the population enrolled contained more dogs with neoplastic diseases than anticipated. The overall cfDNA and nucleosome concentrations were lower than reported for other patient populations,19 20 22 perhaps reflecting a lower disease severity in this cohort compared with others. Despite this, the cfDNA and nucleosome concentrations covered a wide range (~twofold for cfDNA and ~sevenfold for nucleosomes) and provided a solid dataset to assess for evidence of proportional biases between sample types.…”

Section: Discussioncontrasting

confidence: 72%

“…Concentrations of both biomarkers were measured contemporaneously. Concentrations of cfDNA were measured in triplicate using a benchtop fluorimeter (Qubit 3.0 Fluorometer, Life Technologies, Waltham, MA, USA) and relevant reagents (Quant‐It HS dsDNA Kit, Life Technologies, Waltham, MA, USA) according to the manufacturers’ instructions and as previously described 19 22. Mean values of the three measurements were used for subsequent analyses.…”

Section: Methodsmentioning

confidence: 99%

“…In people, concentrations of cfDNA are also increased after myocardial infarction and trauma 17 18. Increased cfDNA concentrations have been identified in dogs with sepsis,19 trauma,20 neoplasia,21 gastric dilation volvulus (GDV) syndrome22 and immune-mediated haemolytic anaemia (IMHA) 23 24…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Effect of sample type on plasma concentrations of cell‐free DNA and nucleosomes in dogs

Goggs

2019

Veterinary Record Open

Self Cite

View full text Add to dashboard Cite

Cell-free DNA (cfDNA) and nucleosomes are two biomarkers of cell death and neutrophil extracellular trap formation that are increased in dogs with sepsis, immune-mediated haemolytic anaemia, cancer and following trauma and have diagnostic and prognostic values. cfDNA and nucleosomes are typically measured in plasma samples using DNA-specific fluorophores and ELISA assays, respectively, but their concentrations may be affected by pre-analytical variables such as sample type. The present study aimed to investigate the influence of sample type on the plasma cfDNA and nucleosome concentrations of a heterogeneous group of dogs presenting to an emergency room. Triplicate samples were collected into K2-ethylenediamine tetraacetic acid, 3.2% citrate and a specialised DNA stabilisation tube (Streck BCT), processed rapidly and frozen for batch analysis. Biomarker concentrations were compared between sample types by calculation of Spearman’s correlation coefficients, and with Deming regression, Bland-Altman plots and the Friedman test. Overall, biomarker concentrations were highly correlated between the three sample types. The most concordant results were obtained using citrate samples and the DNA stabilisation tube. Matched cfDNA concentrations between the different sample types were significantly different but there was no significant difference between the nucleosome concentrations in any of the sample types. The present study suggests that cfDNA and nucleosomes can be successfully measured in various sample types, but distinct sample types do not produce interchangeable results. This argues for use of a consistent sample type within studies and suggests standardisation may be useful for the field.

show abstract

Section: Discussioncontrasting

confidence: 72%

Section: Methodsmentioning

confidence: 99%

See 1 more Smart Citation

Effect of sample type on plasma concentrations of cell‐free DNA and nucleosomes in dogs

Goggs

2019

Veterinary Record Open

Self Cite

View full text Add to dashboard Cite

show abstract

“…Increased concentrations or gene expression of inducers of NETosis (eg, IL-8, TNF-α, and HMGB1) is reported in veterinary patients with a variety of clinical conditions. 56,62,70,200,202,212 This suggests NETosis may occur in various disease states, but it should be recognized that these markers are not specific for NETosis but contribute to numerous inflammatory and cell death pathways. 28,33,163 As discussed above, NETosis is an umbrella term for a variety of molecular pathways that result in DNA release, 94 so it is possible that NETs may be produced without concurrent increases in the concentrations of a particular inducer.…”

Section: Biomarkers Of Netosismentioning

confidence: 99%

Neutrophil-Extracellular Traps, Cell-Free DNA, and Immunothrombosis in Companion Animals: A Review

et al. 2019

Self Cite

View full text Add to dashboard Cite

Immunothrombosis is a potentially beneficial physiological process that aids innate immunity and host defense against pathogen invasion. However, this process can also be damaging when it occurs to excess or in critical blood vessels. Formation of extracellular traps by leukocytes, particularly neutrophils, is central to our understanding of immunothrombosis. In addition to degranulation and phagocytosis, extracellular traps are the third mechanism by which neutrophils combat potential pathogens. These traps consist of extracellular DNA decorated with bactericidal cellular proteins, including elastase, myeloperoxidase, and cathepsins. Neutrophils can release these structures as part of a controlled cell-death process or via a process termed vital NETosis that enables the cells to extrude DNA but remain viable. There is accumulating evidence that NETosis occurs in companion animals, including dogs, horses, and cats, and that it actively contributes to pathogenesis. Numerous studies have been published detailing various methods for identification and quantification of extracellular trap formation, including cell-free DNA, measurements of histones and proteins such as high-mobility group box–1, and techniques involving microscopy and flow cytometry. Here, we outline the present understanding of these phenomena and the mechanisms of extracellular trap formation. We critically review the data regarding measurement of NETosis in companion animals, summarize the existing literature on NETosis in veterinary species, and speculate on what therapeutic options these insights might present to clinicians in the future.

show abstract

“…The half-life of HMGB-1 is ~11 h in people ( 16 ). Concentrations of HMGB-1 are increased in dogs with gastric dilation-volvulus (GDV) ( 17 , 18 ), dogs with sepsis ( 19 ), and correlate with plasma nucleosome concentrations in critically ill horses ( 20 ), suggesting it may provide information about tissue injury in dogs with trauma.…”

Section: Introductionmentioning

confidence: 99%

High Mobility Group Box-1 and Pro-inflammatory Cytokines Are Increased in Dogs After Trauma but Do Not Predict Survival

Goggs

Letendre²

2018

Front. Vet. Sci.

Self Cite

View full text Add to dashboard Cite

Trauma is common in dogs and causes significant morbidity and mortality, but it remains challenging to predict the prognosis of dogs with traumatic injuries. This study aimed to quantify plasma high-mobility group box-1 (HMGB-1) and cytokine concentrations in dogs with moderate-to-severe trauma, and to evaluate the association between these biomarkers and the injury severity and survival to discharge. Using a prospective, observational case-control study design, 49 dogs with an animal trauma triage (ATT) score ≥3 were consecutively enrolled from 07/2015 to 10/2017 and followed to hospital discharge. Dogs <3 kg and those with pre-existing coagulopathies were excluded. Thirty three healthy control dogs were also enrolled. Illness and injury severity scores including the acute patient physiologic and laboratory evaluation (APPLE) were calculated using at-presentation data. Plasma HMGB-1 concentrations were measured by ELISA; concentrations of 13 cytokines were measured using multiplex bead-based assays and separately concentrations of 4 cytokines were measured using a multiplex canine-specific ELISA. All biomarkers were measured in duplicate. Mann-Whitney U tests were used to compare biomarker concentrations between groups and between survivors and non-survivors. Associations between biomarkers were evaluated using Spearman's correlation coefficients. Independent predictors of survival were identified using multivariable logistic regression. Alpha was set at 0.05. Plasma concentrations of HMGB-1, interleukin-6, C-X-C motif chemokine-8, keratinocyte chemoattractant-like, and C-C chemokine ligand-2 were significantly greater in injured dogs vs. controls (all P ≤ 0.011). In univariate analyses, HMGB-1 was significantly greater in non-survivors 46.67 ng/mL (8.94–84.73) compared to survivors 6.03 ng/mL (3.30–15.75), (P = 0.003). Neither HMGB-1 or the cytokines were associated with survival independent of illness severity as measured by the APPLE score, however.

show abstract

Cell-Free DNA, High-Mobility Group Box-1, and Procalcitonin Concentrations in Dogs With Gastric Dilatation–Volvulus Syndrome

Cited by 19 publications

References 41 publications

Effect of sample type on plasma concentrations of cell‐free DNA and nucleosomes in dogs

Effect of sample type on plasma concentrations of cell‐free DNA and nucleosomes in dogs

Neutrophil-Extracellular Traps, Cell-Free DNA, and Immunothrombosis in Companion Animals: A Review

High Mobility Group Box-1 and Pro-inflammatory Cytokines Are Increased in Dogs After Trauma but Do Not Predict Survival

Contact Info

Product

Resources

About