C23 protein meditates bone morphogenetic protein-2-mediated EMT via up-regulation of Erk1/2 and Akt in gastric cancer

Abstract

In our previous study, the epithelial-to-mesenchymal transition (EMT) has been identified to be involved in gastric cancer progression. Notably, nuclear protein C23 and bone morphogenetic protein-2 (BMP2) have been linked into EMT. However, the specific mechanisms underlying BMP2 pathway-mediated EMT are not still unraveled. In this study, we adopted immunohistochemistry and immunoblotting to determine the expression of C23 and BMP2 receptor II (BMPR-II) in 90 gastric cancer samples and cell lines. Subsequently, relevant cell lines were selected to be treated with si-C23 or si-BMPRII and the detection of in vitro assay. Our results revealed that both C23 and BMPRII were aberrantly and constitutively expressed in gastric cancer specimens and cell lines, whose expression was positively associated with metastasis, stage and differentiation, and portended poor survival outcome of gastric cancer patients. In vitro assay validated the increased expression of p-Erk1/2, p-Akt, vimentin, N-cadherin, and MMP2 in BMP2-stimulated MGC803 cells, which was in a dose-dependent manner. By contrast, si-C23 treatment attenuated the BMP2-stimulated expression of p-Erk1/2, p-Akt, vimentin, N-cadherin, and MMP2. Also, the treatment of either si-C23 or si-BMPRII decreased the ability of migration and invasion of MGC803 cells. In conclusion, C23 mediates BMP2-induced EMT progression via the up-regulation of Erk1/2 and Akt signaling pathway in gastric cancer, which indicated both C23 and BMPRII pathway could be recommended as prospective targets or biomarkers to antagonize the progression of gastric cancer.

Hypermethylation of the CHRDL1 promoter induces proliferation and metastasis by activating Akt and Erk in gastric cancer

“…Conversely, over expression of CHRDL1 induced Akt dephosphorylation (Figure 3B). Yang Y et al reported that Akt and Erk can also be phosphorylated by BMPR II [27]. Therefore, we also measured Erk in our experiment.…”

Role of EMT in Metastasis and Therapy Resistance

“…These observations indicated a role of this cell type to regulate oncogenic potential in epithelial cells using a fibroblast-specific TGF-β receptor 2 knockout model (Tgfbr2fspKO). Alternatively, TGF-β ligand BMP-2 induces invasion and cancer stem features via STAT3 activation during colon cancer [ 35 ], and ERK1/2 and AKT during gastric cancer [ 57 , 58 , 59 ]. Tissue scarring depicted by fibrosis, followed by the administration of tumor necrosis factor alpha (TNF-α), leads to mesenchymal marker expression [ 60 ].…”

Roles of nucleolin

“…For instance, transfecting cells with nucleolin-targeted small interfering RNA could result in the inhibition of the EMT phenotypes. 22 Yang et al 23 showed that si-nucleolin treatment attenuated the BMP2-induced expression of p-Erk1/2, p-Akt, vimentin, N-cadherin, and MMP2, leading to decreased migration and invasion of gastric cancer cells. Nucleolin also modulates matrix metalloproteinases (MMPs).…”