C4d deposits in renal allografts are associated with inferior graft outcome

Böhmig, Georg A.; Exner, Markus; Watschinger, Bruno; Wenter, Christian; Wahrmann, Markus; Österreicher, Christoph H.; Säemann, Marcus D.; Mersich, Nicole; Hörl, Walter H.; Zlabinger, Gerhard J.; Regele, Heinz

doi:10.1016/s0041-1345(00)02467-2

Cited by 22 publications

(28 citation statements)

References 4 publications

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“…Regarding C3d-positive biopsies as a different expression of what is now generally excepted as the "C4d-positive type of rejection" (3,6,8,9,(13)(14)(15) implicates the risk of oversimplification. However, to separate both entities, a direct comparison between C3dϩ C4dϪ patients on the one hand and C3dϪ C4dϩ patients and C3dϩC4dϩ recipients on the other hand could indicate whether alternative pathway activation carries a distinct risk.…”

Section: Immunohistochemistrymentioning

confidence: 99%

“…Donor-specific anti-human leukocyte antigen (HLA) type I and II antibodies are implicated in the pathogenesis of this type of rejection (6); however, some investigators have not found a clear relationship between HLA antibodies and acute humoral rejection (7). Patients developing this type of C4d-positive acute rejection (AR) are at high immunologic risk (8) and are more likely to lose their graft (4,5,7,9). Recently, it was shown that the presence of peritubular C4d deposition was a predictor of graft survival independent of the histologic type of AR and classic clinical prognostic factors (8).…”

mentioning

confidence: 99%

“…Most studies have focused on the accumulation of C4 degradation products in PTCs of rejecting kidneys (3,4,8,9,(13)(14)(15), whereas few have investigated the role of C3 deposition. The detection of C3 in tissues is caused by the presence of an intramolecular thioester bond within the C3d region comparable to the one in the C4d region.…”

mentioning

confidence: 99%

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C3d Deposition in Peritubular Capillaries Indicates a Variant of Acute Renal Allograft Rejection Characterized by a Worse Clinical Outcome

et al. 2003

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Section: Immunohistochemistrymentioning

confidence: 99%

mentioning

confidence: 99%

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C3d Deposition in Peritubular Capillaries Indicates a Variant of Acute Renal Allograft Rejection Characterized by a Worse Clinical Outcome

et al. 2003

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“…Over the last decade it has been demonstrated that some of the DSAs detected with SA SP analysis but not with CDC (FC pos/ CDC neg) can activate the complement system [124][125][126][127][128] .…”

Section: Component 1q Sa Sp Binding Assaymentioning

confidence: 99%

Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation

Lobashevsky¹

2014

WJT

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Donor human leukocyte antigen (HLA)-specific antibodies (DSA) play an important role in solid organ transplantation. Preexisting IgG isotype DSA are considered a risk factor for antibody mediated rejection, graft failure or graft loss. The post-transplant development of DSA depends on multiple factors including immunogenicity of mismatched antigens, HLA class Ⅱ typing of the recipient, cytokine gene polymorphisms, and cellular immunoregulatory mechanisms. De novo developed antibodies require special attention because not all DSA have equal clinical significance. Therefore, it is important for transplant clinicians and transplant immunologists to accurately characterize DSA. In this review, the contemporary immunological techniques for detection and characterization of anti-HLA antibodies and their pitfalls are described.

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“…Five renal biopsies negative for HCMV DNA by ISH were also included as negative controls. All renal biopsies were tested for the presence of antibody (Ab)-mediated rejection using a polyclonal peptide-specific anti-C4d antibody (C4dpAb; Biomedica, Vienna, Austria) [Bohmig et al, 2001]. Normal rabbit IgG serum (Sigma) diluted to the same concentration as the anti-C4d Ab was used as a negative control.…”

Section: Immunohistochemistrymentioning

confidence: 99%

Extensive human cytomegalovirus (HCMV) genomic DNA in the renal tubular epithelium early after renal transplantation: Relationship with HCMV DNAemia and long‐term graft function

Emery

Surah

et al. 2009

Journal of Medical Virology

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Human cytomegalovirus (HCMV) infection is associated with a series of direct and indirect effects following renal transplantation. However, the presence of HCMV in the kidney and its relationship with acute rejection and long-term graft function remain to be fully elucidated. Sixty-two biopsies derived from 30 renal transplant recipients with signs of clinical rejection were analyzed for HCMV using a sensitive in situ DNA hybridization method. Biopsies were also subjected to staining with anti-C4d antibodies and an anti-caspase 3 antibody to detect humoral rejection and apoptosis, respectively. In 21 patients, serial serum creatinine levels over 5 years of follow-up were analyzed. HCMV DNA was detected in biopsies from 21/30 (70%) of the patients and 32/62 (52%) of the individual biopsies. HCMV DNA was detected early after transplant and was localized to renal tubule epithelial cells but not associated with apoptosis. HCMV DNAemia developed within 2 weeks of detecting HCMV DNA in the biopsy in 53% of patients. Ninety percent of patients experiencing HCMV disease had HCMV DNA in their biopsy. HCMV DNA was equally distributed between patients with or without histological evidence of acute rejection and was detected more frequently in patients with peritubular C4d deposits. Creatinine levels at 12 months post-transplant were significantly higher in patients with HCMV DNA and remained elevated over the 5 years of follow-up. HCMV DNA is frequently detected in renal tubular epithelial cells early after renal transplantation, precedes DNAemia and is associated with poor long-term graft function.

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C4d deposits in renal allografts are associated with inferior graft outcome

Cited by 22 publications

References 4 publications

C3d Deposition in Peritubular Capillaries Indicates a Variant of Acute Renal Allograft Rejection Characterized by a Worse Clinical Outcome

C3d Deposition in Peritubular Capillaries Indicates a Variant of Acute Renal Allograft Rejection Characterized by a Worse Clinical Outcome

Methodological aspects of anti-human leukocyte antigen antibody analysis in solid organ transplantation

Extensive human cytomegalovirus (HCMV) genomic DNA in the renal tubular epithelium early after renal transplantation: Relationship with HCMV DNAemia and long‐term graft function

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