Ca ionophore-stimulated ion secretion in rabbit ileal mucosa: Relation to actions of cyclic 3′,5′-AMP and carbamylcholine

Bolton, John R.; Field, Michael

doi:10.1007/bf01869947

Cited by 195 publications

(92 citation statements)

References 28 publications

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“…Under these conditions prostaglandin action was found to depend on external Ca2+, in contrast with earlier reports (Bolton & Field, 1977) but in agreement with more recent studies (Hardcastle et al 1984). Thus the fact that histamine-induced intestinal secretion was found to require external Ca2+ and was inhibited by the Ca2+-channel blocker verapamil does not rule out its mediation by prostaglandins.…”

Section: Effect Of Histamine On Fluid Transport In Vivosupporting

confidence: 88%

The secretory actions of histamine in rat small intestine.

Hardcastle

1987

The Journal of Physiology

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SUMMARY1. Histamine caused a dose-dependent rise in the transintestinal potential difference in vivo that was competitively blocked by H1, but not by H2 antagonists. This effect of histamine was also reduced by the cyclo-oxygenase inhibitor indomethacin.2. Histamine induced a net secretion of fluid by the small intestine and this effect was reduced by indomethacin.3, In intestinal sheets histamine increased the potential difference, short-circuit current and tissue resistance. This response was decreased in the absence of Cl-and in the presence of furosemide, suggesting that Cl-secretion was responsible for the observed electrical changes. This was confirmed by direct measurement of ion fluxes which showed an increase in net Cl-secretion together with an inhibition of net Na+ absorption.4. The response to histamine was Ca2+-dependent since it was inhibited by removal of serosal Ca2+ and by verapamil.5. Indomethacin caused a dose-dependent reduction in the response of intestinal sheets to histamine, without affecting the rise in short-circuit current induced by mucosal glucose or serosal prostaglandin E2. Mepacrine also inhibited the response to histamine, but not that to prostaglandin E2.6. It is concluded that histamine induces intestinal secretion by stimulating the production of prostaglandins which then activate the secretory process.

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Section: Effect Of Histamine On Fluid Transport In Vivosupporting

confidence: 88%

The secretory actions of histamine in rat small intestine.

Hardcastle

1987

The Journal of Physiology

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“…These results can also be used to interpret the paradox that choleragen, theophylline and cyclic AMP (Powell, 1974), whilst increasing passive ionic conductance via the paracellular route, also decrease tissue conductance in choleragen and theophylline-induced active secretary state (Field, Fromm, Al-Awqati & Greenough, 1972;Powell et al 1973;Nellans et al 1974;Bolton & Field, 1977). The increase in tissue resistance with theophylline is shown here to occur after an initial decrease in resistance; this decrease is also observed when either, 20 mM-galactose, or triaminopyrimidine is present in the Ringer.…”

Section: Discussionmentioning

confidence: 58%

“…Frizzell, Koch & Schultz (1976) with rabbit colon and Beitch, Beitch & Zadunaisky (1974) with rabbit cornea have shown that cyclic AMP increases active Cl-secretion, short circuit current and also transepithelial conductance in both these 'tight' epithelia. More recently Frizzell (1977) has shown that Ca2+ ionophore A 23187 causes increased short-circuit current due to active Cl-secretion, accompanied by increased electrical conductance across rabbit colon; whereas Bolton & Field (1977) have shown that A 23187 increases short circuit current, and Cl-secretion but decreases transepithelial conductance across rabbit ileum.…”

Section: Introductionmentioning

confidence: 99%

Electrophysiological and electron‐microscopical correlations with fluid and electrolyte secretion in rabbit ileum.

Holman¹,

Naftalin²,

Simmons³

et al. 1979

The Journal of Physiology

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SUMMARY1. The effects of theophylline and triaminopyrimidine on the passive permeability of Na+ and Cl-across sheets of rabbit ileum treated with 0-1 mM-ouabain were examined by determining the NaCl: mannitol dilution potentials, K+: Na+; choline: Na+ and S02-: Cl-biionic potentials. The results indicate (a) that triaminopyrimidine reduces paracellular Na+ and K+ permeability without affecting Cl-permeability and (b) that theophylline increases Cl-permeability without affecting Na+ permeability and (c) that neither theophylline nor triaminopyrimidine interfere with each other's action. This is further evidence consistent with separate routes for paracellular Na+ and Cl-movement. 2. Electrical resistance changes across sheets of actively transporting rabbit ileum were measured as a function of time in various conditions. Theophylline has a biphasic effect on resistance. Initially it decreases resistance from 56 Q cm2 (control) to 40 Q cm2. In the ensuing 30 min, resistance rises to 50 Q cm2; whereas it falls to 30 Q cm2 in controls.When theophylline is present, with triaminopyrimidine, or with galactose, no secondary rise in tissue resistance occurs. The initial decrease in resistance is consistent with a theophylline-dependent increase in Cl-conductance and the secondary rise in resistance may be attributed to collapse of the lateral intercellular space following leakage of NaCl and fluid into the mucosal solution.3. Electron microscopy of glutaraldehyde-fixed tissue confirms the above views, since, with theophylline present, the lateral intercellular spaces are collapsed and with both triaminopyrimidine and theophylline, or both theophylline and 20 mMgalactose present the spaces remain open.4. It is shown in the Discussion that the theophylline-or choleragen-induced increase in passive Cl-permeability of the mucosal border is the only requirement necessary to explain the increase in electrogenic Cl-secretion, the increase in shortcircuit current, as well as neutral secretion of NaCl and net fluid secretion.

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“…Previous studies have suggested that the effects of 5-HT on NaCl absorption (10) and Cl Ϫ secretion (42) are mediated by an increase in the intracellular calcium levels. We thus examined the role of calcium in mediating the effects of 5-HT by using the Ca 2ϩ chelator BAPTA-AM (20 M, 1 h).…”

Section: Effect Of Ca 2ϩ Chelator Bapta-am On 5-ht-mediated Inhibitiomentioning

confidence: 99%

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

Saksena

Gill

Tyagi

et al. 2005

Journal of Biological Chemistry

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The gastrointestinal tract is an important source of the endogenous amine, serotonin, mainly stored in the mucosal enterochromaffin cells and in the enteric nervous system (1). 5-HT1 is continuously released from the enterochromaffin cells in the intestinal mucosa into the portal circulation and the gut lumen in response to a variety of luminal stimuli such as change in pH or osmolarity and mechanical and chemical stimuli (2). 5-HT is an important neurotransmitter and intercellular messenger to modulate gastrointestinal functions (3). 5-HT has been shown to alter gastrointestinal motility (4), alter gastric acid secretion, and enhance intestinal secretions (1, 5). Most of the previous studies regarding the effects of serotonin on ion transport were mainly based on the electrophysiological data including short circuit current measurements and unidirectional fluxes. These studies showed that 5-HT stimulated electrogenic Cl Ϫ secretion and inhibited Na ϩ and Cl Ϫ absorption in the rat colon (6), jejunum (7), ileum (8), and guinea pig ileum (9). 5-HT has also been shown to inhibit the NaClabsorptive process in the rabbit ileum and gall bladder (10) but has no effect in rabbit and guinea pig colon (10, 11). Moreover, Sundaram et al. (12) have demonstrated that 5-HT inhibits Cl Ϫ /HCO 3 Ϫ exchange activity in villus cells and stimulates HCO 3 Ϫ secretion in crypt cells of the rabbit ileum. In contrast, 5-HT appears to have no significant effect on coupled NaCl absorption in the human jejunum (13).5-HT exerts its effects by binding to 5-HT receptors, which are distributed on the neuronal, muscular, and epithelial structures (14). Based on pharmacological studies and molecular cloning (15), the existence of at least seven 5-HT receptors (5-HT1-7) subdivided into 14 subtypes has been discovered. With the exception of 5-HT5 and 5-HT6, 5-HT1 A , 5-HT1 P , 5-HT2, 5-HT3, and 5-HT4 have been reported to be expressed in the gut (16). 5-HT1, 5-HT2, and 5-HT4 receptors are coupled via guanine nucleotide binding (G) proteins to their effectors, whereas 5-HT3 receptor is a ligand-gated ion channel permeable to anions and cations (17).

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Ca ionophore-stimulated ion secretion in rabbit ileal mucosa: Relation to actions of cyclic 3′,5′-AMP and carbamylcholine

Abstract: Addition of the Ca ionophore, A23187 (0.5 gg/ml), to the serosal side of * To whom reprint requests should be addressed.

Cited by 195 publications

References 28 publications

The secretory actions of histamine in rat small intestine.

The secretory actions of histamine in rat small intestine.

Electrophysiological and electron‐microscopical correlations with fluid and electrolyte secretion in rabbit ileum.

Involvement of c-Src and Protein Kinase Cδ in the Inhibition of Cl-/OH- Exchange Activity in Caco-2 Cells by Serotonin

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